Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis

Biotechnology Letters

Volumn 32, Issue 8, 2010, Pages 1077-1082

  Hong, Won Kyung ^a   Kim, Chul Ho ^a   Heo, Sun Yeon ^a   Luo, Lian Hua ^a,b   Oh, Baek Rock ^a,c   Seo, Jeong Woo ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Chonbuk National University   (South Korea)

^c Chonnam National University   (South Korea)

Author keywords

Aldehyde dehydrogenase II; Ethanol production; Glycerol; Hansenula polymorpha; Pyruvate decarboxylase

Indexed keywords

ALDEHYDE DEHYDROGENASE; ALDEHYDE DEHYDROGENASE GENES; ETHANOL PRODUCTION; ETHANOL YIELD; GENES ENCODING; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; GLYCEROL DEHYDROGENASE; HANSENULA POLYMORPHA; KLEBSIELLA PNEUMONIAE; METHYLOTROPHIC YEASTS; PARENT STRAIN; PYRUVATE DECARBOXYLASE; RECOMBINANT STRAINS; ZYMOMONAS MOBILIS;

ALDEHYDES; ENCODING (SYMBOLS); GENE EXPRESSION; GLYCEROL; YEAST;

ETHANOL;

ALCOHOL; ALDEHYDE DEHYDROGENASE; GLYCEROL; PYRUVATE DECARBOXYLASE;

ARTICLE; ENZYMOLOGY; GENETIC ENGINEERING; GENETICS; METABOLISM; METHODOLOGY; PICHIA; PLASMID; ZYMOMONAS; PROCEDURES;

ALDEHYDE DEHYDROGENASE; ETHANOL; GENETIC ENGINEERING; GLYCEROL; PICHIA; PLASMIDS; PYRUVATE DECARBOXYLASE; ZYMOMONAS;

KLEBSIELLA PNEUMONIAE; PICHIA ANGUSTA; ZYMOMONAS MOBILIS;

EID: 77954534280     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0259-z     Document Type: Article

References (18)

1. Cheng KK, Zhang JA, Liu DH, Sun Y, Liu HJ, Yang MD, Xu JM (2007) Pilot-scale production 1, 3-propanediol using Klebsiella pneumoniae. Proc Biochem 42: 740-744.
2. da Silva GP, Mack M, Contiero J (2009) Glycerol: a promising and abundant carbon source for industrial microbiology. Biotechnol Adv 27: 30-39.
3. Dharmadi Y, Murarka A, Gonzalez R (2006) Anaerobic fermentation of glycerol by Escherichia coli: a new platform for metabolic engineering. Biotechnol Bioeng 94: 821-829.
4. Durnin G, Clomburg J, Yeates Z, Alvarez PJ, Zygourakis K, Campbell P, Gonzalez R (2009) Understanding and harnessing the microaerobic metabolism of glycerol in Escherichiacoli. Biotechnol Bioeng 103: 148-161.
5. Gellissen G (ed) (2002) Hansenula polymorpha-biology and applications. Wiley-VCH, Weinheim.
6. Gonzalez R, Murarka A, Dharmadi Y, Yazdani SS (2008) A new model for the anaerobic fermentation of glycerol in enteric bacteria: trunk and auxiliary pathways in Escherichiacoli. Metab Eng 10: 234-245.
7. Guerra E, Chye PP, Berardi E, Piper PW (2005) Hypoxia abolishes transience of the heat-shock response in the methylotrophic yeast Hansenula polymorpha. Microbiology 151: 805-811.
8. Heo JH, Hong WK, Cho EY, Kim MW, Kim JY, Kim CH, Rhee SK, Kang HA (2003) Properties of the Hansenula polymorpha-derived constitutive GAP promoter, assessed using an HAS reporter gene. FEMS Yeast Res 4: 175-184.
9. Hill J, Donald KAG, Griffiths DE (1991) DMSO-enhanced whole cell yeast transformation. Nucleic Acids Res 19: 5791.
10. Ishcuhk OP, Voronovsky AY, Stasyk OV, Gayda GZ, Gonchar MV, Abbas CA, Sibirny AA (2008) Overexpression of pyruvate decarboxylase in the yeast Hansenula polymorpha results in increased ethanol yield in high-temperature fermentation of xylose. FEMS Yeast Res 8: 1164-1174.
11. Johnson DT, Taconi KA (2007) The glycerin glut: options for the value-added conversion of crude glycerol resulting from biodiesel production. Environ Prog 26: 338-348.
12. Murarka A, Dharmadi Y, Yazdani SS, Gonzalez R (2008) Fermentative utilization of glycerol by Escherichia coli and its implications for the production of fuels and chemicals. Appl Environ Microbiol 74: 1124-1135.
13. Postma E, Verduyn C, Scheffers WA, van Dijken JP (1989) Enzymatic analysis of the crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae. Appl Environ Microbiol 55: 468-477.
14. Ryabova OB, Chmil OM, Sibirny AA (2004) Xylose and cellobiose fermentation to ethanol by the thermotolerant methylotrophic yeast Hansenula polymorpha. FEMS Yeast Res 4: 157-164.
15. Slininger PJ, Bothast RJ, Smiley KL (1983) Production of 3-hydroxypropionaldehyde from glycerol. Appl Environ Microbiol 46: 62-67.
16. Sohn JH (1997) Characterization of telomere-associated ARSs (autonomously replicating sequences) and their use for multiple gene integration in Hansenula polymorpha. Ph. D. Thesis, Korea Advanced Institute of Science and Technology, Taejon, Korea.
17. Yazdani SS, Gonzalez R (2007) Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. Curr Opin Biotechnol 18: 213-219.
18. Yazdani SS, Gonzalez R (2008) Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products. Metab Eng 10: 340-351.