In vitro cytocompatibility evaluation of a thermoresponsive NIPAAm-MMA copolymeric surface using L929 cells

Journal of Materials Science: Materials in Medicine

Volumn 21, Issue 5, 2010, Pages 1631-1639

  Varghese, Viji Mary ^a   Raj, Vidya ^b   Sreenivasan, K ^b   Kumary, T V ^a  

^a Sree Chithra Tirunal Institute for Medical Sciences and Technology   (India)

^b Biomedical Technology Wing   (India)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULAR ACTIVITIES; COPOLYMER SOLUTION; COST EFFECTIVE; CROSS-LINKERS; CULTURE SUBSTRATE; CYTOCOMPATIBILITY; CYTOSKELETAL; IMMUNOFLUORESCENT STAINING; IN-VITRO; IN-VIVO; ISO-PROPANOLS; LOWER CRITICAL SOLUTION TEMPERATURE; MAMMALIAN FIBROBLASTS; METHYL METHACRYLATES; MTT ASSAYS; MULTI-LAYERED; N-ISOPROPYLACRYLAMIDES; NEUTRAL RED; STIMULI-RESPONSIVE POLYMER; THERMO-RESPONSIVE; TISSUE CONSTRUCTS; TISSUE CULTURE POLYSTYRENES;

ACRYLIC MONOMERS; AMIDES; BIOMATERIALS; CELL CULTURE; COPOLYMERIZATION; ESTERS; MAMMALS; PLASTIC PRODUCTS; POLYMERS; POLYSTYRENES; SCAFFOLDS; TISSUE; TISSUE CULTURE;

TISSUE ENGINEERING;

2 PROPANOL; COPOLYMER; POLY(METHYL METHACRYLATE); POLY(N ISOPROPYLACRYLAMIDE); POLYSTYRENE; THYMIDINE;

ANIMAL CELL; ARTICLE; CELL ACTIVITY; CELL ADHESION; CELL VIABILITY; CELLULAR, SUBCELLULAR AND MOLECULAR BIOLOGICAL PHENOMENA AND FUNCTIONS; CYTOCOMPATIBILITY; CYTOSKELETON; DRUG COATING; DRUG UPTAKE; IMMUNOFLUORESCENCE; IN VITRO STUDY; INCUBATION TEMPERATURE; MOUSE; NITROBLUE TETRAZOLIUM TEST; NONHUMAN; PRIORITY JOURNAL; STAINING; SURFACE PROPERTY; THERMODYNAMICS; TISSUE CULTURE;

ACRYLAMIDES; ANIMALS; BIOCOMPATIBLE MATERIALS; CELLS; FIBROBLASTS; L CELLS (CELL LINE); METHYLMETHACRYLATE; MICE; POLYMERS; TEMPERATURE; TISSUE ENGINEERING; WATER;

MAMMALIA;

EID: 77954533423     PISSN: 09574530     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10856-010-3992-x     Document Type: Article

References (26)

1. Hirose M, Kwon OH, Yamato M, Kikuchi A, Okano T. Creation of designed shape cell sheets that are noninvasively harvested and moved onto another surface. Biomacromolecules. 2000;1: 377-381
2. Okano T, Yamada N, Sakai H, Sakurai Y. A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(n-isopropylacrylamide). J Biomed Mater Res. 1993;27:1243-1251
3. von Recum H, Kikuchi A, Okuhara M, Sakurai Y, Okano T, Kim SW. Retinal pigmented epithelium cultures on thermally responsive polymer porous substrates. J Biomater Sci Polym Ed. 1998;9:1241-1253
4. Kushida A, Yamato M, Konno C, Kikuchi A, Sakurai Y, Okano T. Decrease in culture temperature releases monolayer endothelial cell sheets together with deposited fibronectin matrix from temperature-responsive culture surfaces. J Biomed Mater Res. 1999;45:355-362
5. Takezawa T, Mori Y, Yoshizato K. Cell culture on a thermoresponsive polymer surface. Biotechnology (N Y). 1990;8:854-856
6. Tsuda Y, Kikuchi A, Yamato M, Nakao A, Sakurai Y, Umezu M, et al. The use of patterned dual thermoresponsive surfaces for the collective recovery as co-cultured cell sheets. Biomaterials. 2005;26:1885-1893
7. Liu H, Ito Y. Gradient micropattern immobilization of a thermoresponsive polymer to investigate its effect on cell behavior. J Biomed Mater Res A. 2003;67:1424-1429
8. Quan CY, Wei H, Sun YX, Cheng SX, Shen K, Gu ZW, et al. Polyethyleneimine modified biocompatible poly(n-isopropylacrylamide)- based nanogels for drug delivery. J Nanosci Nanotechnol. 2008;8:2377-2384
9. Dai F, Tang L, Yang J, Zhao X, Liu W, Chen G, et al. Fast thermoresponsive bab-type hema/nipaam triblock copolymer solutions for embolization of abnormal blood vessels. J Mater Sci Mater Med. 2009;20:967-974
10. Zhang XZ, Wu DQ, Chu CC. Synthesis, characterization and controlled drug release of thermosensitive ipn-pnipaam hydrogels. Biomaterials. 2004;25:3793-3805
11. Repetto G, del Peso A, Zurita JL. Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nat Protoc. 2008; 3:1125-1131
12. Prasad CK, Muraleedharan CV, Krishnan LK. Bio-mimetic composite matrix that promotes endothelial cell growth for modification of biomaterial surface. J Biomed Mater Res A. 2007;80:644-654
13. Shivakumar K, Nair RR, Valiathan MS. Paradoxical effect of cerium on collagen synthesis in cardiac fibroblasts. J Mol Cell Cardiol. 1992;24:775-780
14. Freshney RI. Culture of animal cells: a manual of basic technique. 4th ed. New York: Wiley-Liss; 2000.
15. Wang LS, Chow PY, Phan TT, Lim IJ, Yang YY. Fabrication and characterization of nanostructured and thermosensitive polymer membranes for wound healing and cell grafting. Adv Funct Mater. 2006;16:1171-1178
16. Tsuda Y, Kikuchi A, Yamato M, Sakurai Y, Umezu M, Okano T. Control of cell adhesion and detachment using temperature and thermoresponsive copolymer grafted culture surfaces. J Biomed Mater Res A. 2004;69:70-78
17. Gao J, Frisken BJ. Influence of secondary components on the synthesis of self-cross-linked n-isopropylacrylamide microgels. Langmuir. 2005;21:545-551
18. Kushida A, Yamato M, Kikuchi A, Okano T. Two-dimensional manipulation of differentiated madin-darby canine kidney (mdck) cell sheets: the noninvasive harvest from temperature-responsive culture dishes and transfer to other surfaces. J Biomed Mater Res. 2001;54:37-46.
19. Anil Kumar PR, Sreenivasan K, Kumary TV. Alternate method for grafting thermoresponsive polymer for transferring in vitro cell sheet structures. J Appl Polym Sci. 2007;105:2245-2251
20. Huang HH, Ho CT, Lee TH, Lee TL, Liao KK, Chen FL. Effect of surface roughness of ground titanium on initial cell adhesion. Biomol Eng. 2004;21:93-97
21. Marinucci L, Balloni S, Becchetti E, Belcastro S, Guerra M, Calvitti M, et al. Effect of titanium surface roughness on human osteoblast proliferation and gene expression in vitro. Int J Oral Maxillofac Implants. 2006;21:719-725
22. Shimizu T, Yamato M, Kikuchi A, Okano T. Two-dimensional manipulation of cardiac myocyte sheets utilizing temperatureresponsive culture dishes augments the pulsatile amplitude. Tissue Eng. 2001;7:141-151
23. Shiroyanagi Y, Yamato M, Yamazaki Y, Toma H, Okano T. Transplantable urothelial cell sheets harvested noninvasively from temperature-responsive culture surfaces by reducing temperature. Tissue Eng. 2003;9:1005-1012
24. Canavan HE, Cheng X, Graham DJ, Ratner BD, Castner DG. Cell sheet detachment affects the extracellular matrix: A surface science study comparing thermal liftoff, enzymatic, and mechanical methods. J Biomed Mater Res A. 2005;75:1-13.
25. Cheng C, Wei H, Zhang XZ, Cheng SX, Zhuo RX. Thermotriggered and biotinylated biotin-p(nipaam-co-hmaam)-b-pmma micelles for controlled drug release. J Biomed Mater Res A. 2009;88:814-822
26. Lin CL, Chiu WY, Lee CF. Preparation of thermoresponsive core-shell copolymer latex with potential use in drug targeting. J Colloid Interface Sci. 2005;290:397-405.