New applications of pHluorin - Measuring intracellular pH of prototrophic yeasts and determining changes in the buffering capacity of strains with affected potassium homeostasis

Yeast

Volumn 27, Issue 6, 2010, Pages 317-325

  Marešová, Lydie ^a   Hošková, Barbora ^b   Urbánková, Eva ^b   Chaloupka, Roman ^b   Sychrová, Hana ^a  

^a INSTITUTE OF PHYSIOLOGY   (Czech Republic)

^b CHARLES UNIVERSITY   (Czech Republic)

Author keywords

Alkali metal cation transporters; Fluorescence measurements; Microplate reader; Pma1 ATPase

Indexed keywords

BUFFER; GLUCOSE; GREEN FLUORESCENT PROTEIN; HYDROGEN POTASSIUM ADENOSINE TRIPHOSPHATASE; PHLUORIN; POTASSIUM; UNCLASSIFIED DRUG;

ACIDIFICATION; ARTICLE; CELL CULTURE; CELL GROWTH; CELL MEMBRANE; CELL PH; CONTROLLED STUDY; CYTOSOL; FLUORESCENCE MICROSCOPY; NONHUMAN; PLASMID; POTASSIUM BALANCE; POTASSIUM TRANSPORT; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; YEAST;

BUFFERS; CELL MEMBRANE; CYTOSOL; GREEN FLUORESCENT PROTEINS; HOMEOSTASIS; HYDROGEN-ION CONCENTRATION; PLASMIDS; POTASSIUM; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SPECIES SPECIFICITY;

EID: 77954514794     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.1755     Document Type: Article

References (38)

1. Alberty RA. 2007. Effects of pH in rapid-equilibrium enzyme kinetics. J Phys Chem B 111: 14064-14068.
2. Arroyo-Lopez FN, Orlic S, Querol A, Barrio E. 2009. Effects of temperature, pH and sugar concentration on the growth parameters of Saccharomyces cerevisiae, S. kudriavzevii and their interspecific hybrid. Int J Food Microbiol 131: 120-127.
3. Banuelos MA, Sychrova H, Bleykasten-Grosshans C, et al. 1998. The Nha1 antiporter of Saccharomyces cerevisiae mediates sodium and potassium efflux. Microbiology 144: 2749-2758.
4. Bertl A, Ramos J, Ludwig J, et al. 2003. Characterization of potassium transport in wild-type and isogenic yeast strains carrying all combinations of trk1, trk2 and tok1 null mutations. Mol Microbiol 47: 767-780.
5. Breeuwer P, Abee T. 2000. Assessment of the intracellular pH of immobilized and continuously perfused yeast cells employing fluorescence ratio imaging analysis. J Microbiol Methods 39: 253-264.
6. + exchanger Nhx1 regulates cellular pH to control vesicle trafficking. Mol Biol Cell 16: 1396-1405.
7. + antiporter from rice. Plant Cell Physiol 45: 146-159.
8. Gillies RJ, Ugurbil K, den Hollander JA, Shulman RG. 1981. 31PNMR studies of intracellular pH and phosphate metabolism during cell division cycle of Saccharomyces cerevisiae. Proc Natl Acad Sci USA 78: 2125-2129.
9. Güldener U, Heck S, Fielder T, et al. 1996. A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24: 2519-2524.
10. Haworth RS, Fliegel L. 1993. Intracellular pH in Schizosaccharomyces pombe - comparison with Saccharomyces cerevisiae. Mol Cell Biochem 124: 131-140.
11. Karagiannis J, Young PG. 2001. Intracellular pH homeostasis during cell-cycle progression and growth state transition in Schizosaccharomyces pombe. J Cell Sci 114: 2929-2941.
12. Kresnowati MT, Suarez-Mendez C, Groothuizen MK, et al. 2007. Measurement of fast dynamic intracellular pH in Saccharomyces cerevisiae using benzoic acid pulse. Biotechnol Bioeng 97: 86-98.
13. Kresnowati MT, Suarez-Mendez CM, van Winden WA, et al. 2008. Quantitative physiological study of the fast dynamics in the intracellular pH of Saccharomyces cerevisiae in response to glucose and ethanol pulses. Metab Eng 10: 39-54.
14. Lanz E, Slavik J, Kotyk A. 1999. 2' 7'-bis-(2-carboxyethyl)-5(6)- carboxyfluorescein as a dual-emission fluorescent indicator of intracellular pH suitable for argon laser confocal microscopy. Folia Microbiol (Praha) 44: 429-434.
15. Lasorsa FM, Scarcia P, Erdmann R, et al. 2004. The yeast peroxisomal adenine nucleotide transporter: characterization of two transport modes and involvement in δ-pH formation across peroxisomal membranes. Biochem J 381: 581-585.
16. +-ATPase leads to glucose-dependent activation. J Biol Chem 282: 35471-35481.
17. Maresova L, Muend S, Zhang YQ, et al. 2009. Membrane hyperpolarization drives cation influx and fungicidal activity of amiodarone. J Biol Chem 284: 2795-2802.
18. Maresova L, Sychrova H. 2007. Applications of a microplate reader in yeast physiology research. Biotechniques 43: 667-672.
19. Maresova L, Urbankova E, Gaskova D, Sychrova H. 2006. Measurements of plasma membrane potential changes in Saccharomyces cerevisiae cells reveal the importance of the Tok1 channel in membrane potential maintenance. FEMS Yeast Res 6: 1039-1046.
20. Martinez-Munoz GA, Kane P. 2008. Vacuolar and plasma membrane proton pumps collaborate to achieve cytosolic pH homeostasis in yeast. J Biol Chem 283: 20309-20319.
21. McCusker JH, Perlin DS, Haber JE. 1987. Pleiotropic plasma membrane ATPase mutations of Saccharomyces cerevisiae. Mol Cell Biol 7: 4082-4088.
22. Mellman I. 1992. The importance of being acid: the role of acidification in intracellular membrane traffic. J Exp Biol 172: 39-45.
23. Miesenböck G, De Angelis DA, Rothman JE. 1998. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394: 192-195.
24. + influx. J Cell Sci 117: 2309-2320.
25. Orij R, Postmus J, Ter Beek A, et al. 2009. In vivo measurement of cytosolic and mitochondrial pH using a pH-sensitive GFP derivative in Saccharomyces cerevisiae reveals a relation between intracellular pH and growth. Microbiology 155: 268-278.
26. + exchangers of mammalian cells. J Biol Chem 272: 22373-22376.
27. Pena A, Ramirez J, Rosas G, Calahorra M. 1995. Proton pumping and the internal pH of yeast cells, measured with pyranine introduced by electroporation. J Bacteriol 177: 1017-1022.
28. +-ATPase of hygromycin B-resistant pma1 mutants from Saccharomyces cerevisiae. J Biol Chem 264: 21857-21864.
29. Roos A, Boron WF. 1981. Intracellular pH. Physiol Rev 61: 296-434.
30. Rottenberg H. 1979. The measurement of membrane potential and δ-pH in cells, organelles, and vesicles. Methods Enzymol 55: 547-569.
31. Serrano R. 1983. In vivo glucose activation of the yeast plasma membrane ATPase. FEBS Lett 156: 11-14.
32. Sigler K, Kotyk A, Knotkova A, Opekarova M. 1981. Processes involved in the creation of buffering capacity and in substrateinduced proton extrusion in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 643: 583-592.
33. + antiporter carboxy-terminal tail reveals residues required for function in cell cycle. FEBS Lett 545: 239-245.
34. Stevens HC, Nichols JW. 2007. The proton electrochemical gradient across the plasma membrane of yeast is necessary for phospholipid flip. J Biol Chem 282: 17563-17567.
35. Sureshkumar GK, Mutharasan R. 1994. Intracellular pH responses of hybridoma and yeast to substrate addition and acid challenge. Ann NY Acad Sci 745: 106-121.
36. Sychrova H, Ramirez J, Pena A. 1999. Involvement of Nha1 antiporter in regulation of intracellular pH in Saccharomyces cerevisiae. FEMS Microbiol Lett 171: 167-172.
37. Veine DM, Arscott LD, Williams CH Jr. 1998. Redox potentials for yeast, Escherichia coli and human glutathione reductase relative to the NAD+/NADH redox couple: enzyme forms active in catalysis. Biochemistry 37: 15575-15582.
38. Whitten ST, Wooll JO, Razeghifard R, et al. 2001. The origin of pH-dependent changes in m-values for the denaturant-induced unfolding of proteins. J Mol Biol 309: 1165-1175.