Mass spectrometric identification of dystrophin isoform Dp427 by on-membrane digestion of sarcolemma from skeletal muscle

Analytical Biochemistry

Volumn 404, Issue 2, 2010, Pages 197-203

  Lewis, Caroline ^a   Ohlendieck, Kay ^a  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

Author keywords

Dystrophin; Muscle proteomics; On membrane digestion; Sarcoglycan; Sarcolemma; Syntrophin

Indexed keywords

ELECTROPHORESIS; GLYCOPROTEINS; MASS SPECTROMETERS; MASS SPECTROMETRY; MEMBRANES; MUSCLE; PROTEOMICS;

DIHYDROPYRIDINE RECEPTORS; DYSTROPHIN; MASS SPECTROMETRIC IDENTIFICATION; MEMBRANE-ASSOCIATED PROTEINS; SARCOGLYCAN; SARCOLEMMA; SYNTROPHIN; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

PROTEINS;

DYSTROPHIN; SARCOGLYCAN; SYNTROPHIN; CALCIUM; GLYCOPROTEIN; ISOPROTEIN;

ANIMAL TISSUE; ARTICLE; GEL ELECTROPHORESIS; INSTRUMENT; LEG MUSCLE; LIQUID CHROMATOGRAPHY; MASS SPECTROMETER; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; RABBIT; SARCOLEMMA; SKELETAL MUSCLE; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; METABOLISM; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES;

AMINO ACID SEQUENCE; ANIMALS; CALCIUM; DYSTROPHIN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOPROTEINS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MUSCLE, SKELETAL; PROTEIN ISOFORMS; PROTEOMICS; RABBITS; SARCOLEMMA;

EID: 77954457960     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.05.017     Document Type: Article

References (28)

1. Campbell K.P. Three muscular dystrophies: loss of cytoskeleton-extracellular matrix linkage. Cell 1995, 80:675-679.
2. Ohlendieck K. Towards an understanding of the dystrophin-glycoprotein complex: linkage between the extracellular matrix and the subsarcolemmal membrane cytoskeleton. Eur. J. Cell Biol. 1996, 69:1-10.
3. Ervasti J.M., Sonnemann K.J. Biology of the striated muscle dystrophin-glycoprotein complex. Int. Rev. Cytol. 2008, 265:191-225.
4. Koenig M., Kunkel L.M. Detailed analysis of the repeat domain of dystrophin reveals four potential hinge segments that may confer flexibility. J. Biol. Chem. 1990, 265:4560-4566.
5. Ohlendieck K., Matsumura K., Ionasescu V.V., Towbin J.A., Bosch P., Weinstein S.L., Sernett S.W., Campbell K.P. Duchenne muscular dystrophy: deficiency of dystrophin-associated proteins in the sarcolemma. Neurology 1993, 43:795-800.
6. Ge Y., Molloy M.P., Chamberlain J.S., Andrews P.C. Proteomic analysis of mdx skeletal muscle: great reduction of adenylate kinase 1 expression and enzymatic activity. Proteomics 2003, 3:1895-1903.
7. 2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle. Eur. J. Biochem. 2004, 271:3943-3952.
8. Doran P., Dowling P., Donoghue P., Buffini M., Ohlendieck K. Reduced expression of regucalcin in young and aged mdx diaphragm indicates abnormal cytosolic calcium handling in dystrophin-deficient muscle. Biochim. Biophys. Acta 2006, 1764:773-785.
9. Doran P., Martin G., Dowling P., Jockusch H., Ohlendieck K. Proteome analysis of the dystrophin-deficient MDX diaphragm reveals a drastic increase in the heat shock protein cvHSP. Proteomics 2006, 6:4610-4621.
10. Lewis C., Carberry S., Ohlendieck K. Proteomic profiling of X-linked muscular dystrophy. J. Muscle Res. Cell Motil. 2009, 30:267-279.
11. Goerg A., Weiss W., Dunn M.J. Current two-dimensional electrophoresis technology for proteomics. Proteomics 2004, 4:3665-3685.
12. Wittmann-Liebold B., Graack H.R., Pohl T. Two-dimensional gel electrophoresis as tool for proteomics studies in combination with protein identification by mass spectrometry. Proteomics 2006, 6:4688-4703.
13. Rabilloud T., Vaezzadeh A.R., Potier N., Lelong C., Leize-Wagner E., Chevallet M. Power and limitations of electrophoretic separations in proteomics strategies. Mass Spectrom. Rev. 2009, 28:816-843.
14. Luque-Garcia J.L., Zhou G., Sun T.T., Neubert T.A. Use of nitrocellulose membranes for protein characterization by matrix-assisted laser desorption/ionization mass spectrometry. Anal. Chem. 2006, 78:5102-5108.
15. Luque-Garcia J.L., Zhou G., Spellman D.S., Tung-Tien S., Neubert T.A. Analysis of electroblotted proteins by mass spectrometry: protein identification after Western blotting. Mol. Cell. Proteomics 2008, 7:308-314.
16. Aebersold R.H., Leavitt J., Saavedra R.A., Hood L.E., Kent S.B. Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose. Proc. Natl. Acad. Sci. USA 1987, 84:6970-6974.
17. Luque-Garcia J.L., Neubert T.A. On-membrane tryptic digestion of proteins for mass spectrometry analysis. Methods Mol. Biol. 2009, 536:331-341.
18. Ohlendieck K., Ervasti J.M., Snook J.B., Campbell K.P. Dystrophin glycoprotein complex is highly enriched in skeletal muscle sarcolemma. J. Cell Biol. 1991, 112:135-148.
19. Ohlendieck K., Campbell K.P. Dystrophin constitutes 5% of membrane cytoskeleton in skeletal muscle. FEBS Lett. 1991, 283:230-234.
20. Ohlendieck K. Membrane cytoskeletal characterisation of utrophin in highly purified sarcolemma vesicles. Biochim. Biophys. Acta 1996, 1283:215-222.
21. Ervasti J.M., Ohlendieck K., Kahl S.D., Gaver M.G., Campbell K.P. Deficiency of a glycoprotein component of the dystrophin complex in dystrophic muscle. Nature 1990, 345:315-319.
22. Shevchenko A., Tomas H., Havlis J., Olsen J.V., Mann M. In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat. Protoc. 2006, 1:2856-2860.
23. Perkins D.N., Pappin D.J., Creasy D.M., Cottrell J.S. Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 1999, 20:3551-3567.
24. Koenig T., Menze B.H., Kirchner M., Monigatti F., Parker K.C., Steen J.J., Hamprecht F.A., Steen H. Robust prediction of the Mascot score for an improved quality assessment in mass spectrometric proteomics. J. Proteome Res. 2008, 7:3708-3717.
25. de Hoog C.L., Mann M. Proteomics. Annu. Rev. Genomics Hum. Genet. 2004, 5:267-293.
26. Isfort R.J. Proteomic analysis of striated muscle. J. Chromatogr. B 2002, 771:155-165.
27. Ohlendieck K. Proteomics of skeletal muscle differentiation, neuromuscular disorders, and aging. Expert Rev. Proteomics 2010, 7:283-296.
28. Doran P., Donoghue P., O'Connell K., Gannon J., Ohlendieck K. Proteomics of skeletal muscle aging. Proteomics 2009, 9:989-1003.