Comparison of MGIT 960 & pyrazinamidase activity assay for pyrazinamide susceptibility testing of Mycobacterium tuberculosis

Indian Journal of Medical Research

Volumn 132, Issue 7, 2010, Pages 72-76

  Sharma, Babita ^a   Pal, Nita ^a   Malhotra, Bharti ^a   Vyas, Leela ^a   Rishi, Suman ^a  

^a SMS MEDICAL COLLEGE   (India)

Author keywords

MGIT 960; Mycobacterium tuberculosis; Pyrazinamide

Indexed keywords

BACTERIAL ENZYME; PYRAZINAMIDASE; PYRAZINAMIDE; UNCLASSIFIED DRUG; AMIDASE; PYRAZINAMIDE DEAMIDASE; TUBERCULOSTATIC AGENT;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; INTERMETHOD COMPARISON; MGIT 960 ENZYME ASSAY; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; SENSITIVITY AND SPECIFICITY; ANTIBIOTIC RESISTANCE; CHEMISTRY; COMPARATIVE STUDY; CULTURE MEDIUM; DRUG EFFECT; EVALUATION; METABOLISM; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PH; TUBERCULOSIS;

AMIDOHYDROLASES; ANTITUBERCULAR AGENTS; CULTURE MEDIA; DRUG RESISTANCE, MICROBIAL; HYDROGEN-ION CONCENTRATION; MICROBIAL SENSITIVITY TESTS; MYCOBACTERIUM TUBERCULOSIS; PYRAZINAMIDE; TUBERCULOSIS;

EID: 77954451294     PISSN: 09715916     EISSN: 09715916     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Konno K, Feldman FM, McDermott W. Pyrazinamide susceptibility and amidase activity of tubercle bacilli. Am Rev Respir Dis 1967; 95: 461-9.
2. National Committee for Clinical Laboratory Standards. Antimycobacterial susceptibility testing for Mycobacterium tuberculosis, Tentative standard M24-T. Wayne, Pa: National Committee for Clinical Laboratory Standards; 1995.
3. Woods G L. Susceptibility testing for mycobacteria. Clin Infect Dis 2000; 31: 1209-15.
4. McDermott W, Tompsett R. Activation of pyrazinamide and nicotinamide in acidic environments in vitro. Am Rev Tuberc 1954; 70: 748-54.
5. Zhang Y, Permar S, Sun Z. Condition that may affect the results of Mycobacterium tuberculosis susceptibility testing to pyrezinamide. J Med Microbiol 2002; 50: 34-50
6. Kent PT, Kubica GP. Public health mycobacteriology. A guide for the level III laboratory. Atlanta GA: US Health Service Centers for Disease Control; 1985.
7. Wayne LG. Simple pyrazinamidase and urease tests for routine identifcation of mycobacteria. Am Rev Respir Dis 1974; 109: 147-51.
8. McClatchy J K, Tsang AY, Cernich MS. Use of pyrazinamidase activity in Mycobacterium tuberculosis as a rapid method for determination of pyrazinamide susceptibility. Antimicrob Agents Chemother 1981; 20: 556-57.
9. Singh P, Wesley C, Jadaun GPS, Malonia SK, Das R, Upadhyay P, et al. Comparative evaluation of Lowenstein-Jensen proportion method, BacT/ALERT 3D system, and enzymatic pyrazinamidase assay for pyrazinamide susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol 2007; 45: 76-80.
10. Pfyffer GE, Palicova F, Rüsch-Gerdes S. Testing of susceptibility to pyrazinamide with the nonradiometric BACTEC MGIT 960 system. J Clin Microbiol 2002; 40: 1670-4.
11. Salfnger M, and Heifets LB. Determination of pyrazinamide MICs for Mycobacterium tuberculosis at different pHs by the radiometric method. Antimicrob Agents Chemother 1988; 32: 1002-4.
12. Mitchision DA. The action of anti-tuberculosis drugs in short course chemotherapy. Tubercle 1985; 66: 219-2.
13. Heiftet L, Lindolm Levy. Pyrazinamide sterilizing activity in vitro against semi dormant Mycobacterium tuberculosis bacterial population. Am Rev Respir Dis 1992; 145: 1223-5.
14. Miller MA, Thibert L, Desjardins F, Siddiqi SH, Dascal A. Testing of susceptibility of Mycobacterium tuberculosis to pyrazinamide: comparison of BACTEC method with pyrazinamidase assay. J Clin Microbiol 1995; 33: 2468-70.
15. Trivedi SS, Desai SG. Pyrazinamidase activity of Mycobacterium tuberculosis - a test of sensitivity to pyrazinamide. Tubercle 1987; 68: 221-4.
16. Lu R, Floyd C, de la Maza LM, Peterson EM. Pyrazinamide (PZA) susceptibility testing of Mycobacterium tuberculosis by two rapid methods, abstr. 883. In: Program and abstracts of the Twenty-Ninth Interscience Conference on Antimicrobial Agents and Chemotherapy. Washington, D.C: American Society for Microbiology; 1989.
17. Krishnamurthy A, Almeida D, Rodrigues C, Mehta A. Comparison of pyrazinamide drug susceptibility of Mycobacterium tuberculosis by radiometric BACTEC and enzymatic pyrazinamidase assay. Indian J Med Microbiol 2004; 22: 166-8.
18. Aono A, Hirano K, Hamasaki S, Abe C. Evaluation of BACTEC MGIT 960 PZA medium for susceptibility testing of Mycobacterium tuberculosis to pyrazinamide (PZA): compared with the results of pyrazinamidase assay and Kyokuto PZA test. Diagn Microbiol Infect Dis 2002; 44: 347-52.
19. Butler WR, Kilburn JO. Susceptibility of Mycobacterium tuberculosis to pyrazinamide and its relationship to pyrazinamidase activity. Antimicrob Agents Chemother 1983; 24: 600-1.
20. Raynaud C, Laneelle MA, Senaratne RH, Draper P, Laneelle G, Daffe M. Mechanisms of pyrazinamide resistance in mycobacteria: importance of lack of uptake in addition to lack of pyrazinamidase activity. Microbiology 1999; 145: 1359-67.
21. Good RC, Silcox VA, Kilbium JO, Plikayto BD. Identifcation and drug susceptibility test results for mycobacterial species. Clin Microbiol Newslett 1985; 7: 133-6.
22. Tarnok I, Rohrcheidt E. Biochemical background of some enzymetic tests used for the differentiation of mycobacteria. Tubercle 1976; 57: 145-50.
23. Heifets LB, Iseman MD. Radiometric method for testing susceptibility of mycobacteria to pyrazinamide in 7H12 broth. J Clin Microbiol 1985; 21: 200-4.
24. Kantor IN, Amadio GE, Catalini EA, Dilonardo M, Dominguez L, Kusso T, et al. Pyrazinamidase activity and susceptibility to pyrazinamide in clinical isolates of Mycobacterium tuberculosis. Pyrazinamide susceptibility of Mycobacterium tuberculosis. Bull Int Union Tuberc 1986; 61: 60-1.