Automated counting of nucleated cells in equine synovial fluid without and with hyaluronidase pretreatment

Veterinary Clinical Pathology

Volumn 39, Issue 1, 2010, Pages 83-89

  Ekmann, Anita ^a   Rigdal, Marie Louise ^b   Gröndahl, Gittan ^c  

^a ATG Horse Clinic Ltd   (Sweden)

^b Boule Medical AB   (Sweden)

^c NATIONAL VETERINARY INSTITUTE   (Sweden)

Author keywords

Equine; Hyaluronidase; Impedance counter; Nucleated cell counts; Synovial fluid; Viscosity

Indexed keywords

HYALURONOGLUCOSAMINIDASE;

ANIMAL; ANIMAL DISEASE; ARTICLE; AUTOMATION; CYTOLOGY; FLOW CYTOMETRY; HORSE; INSTRUMENTATION; METABOLISM; SENSITIVITY AND SPECIFICITY; SYNOVIAL FLUID;

ANIMALS; AUTOMATION; FLOW CYTOMETRY; HORSES; HYALURONOGLUCOSAMINIDASE; SENSITIVITY AND SPECIFICITY; SYNOVIAL FLUID;

EQUIDAE;

EID: 77954379678     PISSN: 02756382     EISSN: 1939165X     Source Type: Journal    
DOI: 10.1111/j.1939-165X.2009.00203.x     Document Type: Article

References (23)

1. McIlwraith CW. Synovial fluid analysis in the diagnosis of equine joint disease. Vet Clin North Am Equine Pract 1980, 2:44-48.
2. Tew WP, Hotchkiss RN. Synovial fluid analysis and equine joint disorders. J Equine Vet Sci 1981, 1:163-170.
3. La-Pointe JM, Laverty S, Lavoie JP. Septic arthritis in 15 standardbred racehorses after intra-articular injection. Equine Vet J 1992, 24:430-434.
4. Mahaffey EA. Synovial fluid. Cytology and Hematology of the Horse 1992, 153-161. Pratt PW. ed, 1st ed, Goleta, CA, Am Vet Pub
5. Pascual E, Jovani V. Synovial fluid analysis. Best Pract Res Clin Rheumatol 2005, 19:371-386.
6. de Jonge R, Brouwer R, Smit M. Automated counting of white blood cells in synovial fluid. Rheumatology 2004, 43:170-173.
7. Sugiuchi H, Ando Y, Manabe M. Measurement of total and differential white blood cell counts in synovial fluid by means of an automated hematology analyzer. J Lab Clin Med 2005, 146:36-42.
8. Schumacher HR, Sieck MS, Rothfuss S. Reproducibility of synovial fluid analyses: a study among four laboratories. Arthritis Rheum 1986, 29:770-774.
9. Hasselbacher P. Variation in synovial fluid analysis by hospital laboratories. Arthritis Rheum 1987, 30:637-642.
10. Dieppe PA, Crocker PR, Corke CF, Doyle DV, Hiskisson EC, Willoughby DA. Synovial fluid crystals. Q J Med 1979, 48:533-553.
11. Vincent J, Korn JH, Podewell C, Tully E. Synovial fluid pseudoleukocytosis. Arthritis Rheum 1980, 23:1399-1400.
12. Salinas M, Rosas J, Iborra J, Manero H, Pascual E. Comparison of manual and automated cell counts in EDTA preserved synovial fluids. Storage has little influence on the results. Ann Rheum Dis 1997, 56:622-626.
13. Atilola MAO, Lumsden JH, Rooke F. A comparison of manual and electronic counting for total nucleated cell counts on synovial fluid from canine stifle joints. Can J Vet Res 1986, 50:282-284.
14. Kreil G. Hyaluronidases-A group of neglected enzymes. Protein Sci 1995, 4:1666-1669.
15. Linn FC, Radin EL. Lubrication of animal joints: III. The effect of certain chemical alterations of the cartilage and lubricant. Arthritis Rheum 1968, 11:674-682.
16. Palmer DG. Total leukocyte enumeration in pathologic synovial fluids. Am J Clin Pathol 1968, 49:812-814.
17. Moreno MJ, Clayburne G, Schumacher RH. Processing of noninflammatory synovial fluids with hyaluronidase for cytospin preparations improves the accuracy of differential counts. Diagn Cytopathol 2000, 22:256-258.
18. David F. Bauer. Constructing confidence sets using rank statistics. J Am Stat Assoc 1972, 67:687-690.
19. Hollander M, Wolfe DA. Nonparametric Statistical Methods 1999, 54. 2nd ed, New York, NY, John Wiley & Sons
20. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986, 1:307-310.
21. Caron JP. Osteoarthritis. Diagnosis and Management of Lameness in the Horse 2003, 572-591. Ross MWDyson SJ. eds, 1st ed, Philadelphia, PA, WB Saunders
22. Bourgès-Abella NH, Reynolds BS, Geffré A, Braun JP, Trumell C. Validation of 20 μL-microcapillary cephalic samples for haematology testing of feline blood. Comparison with conventional jugular samples. J Vet Diagn Invest 2009, 21:364-368.
23. Persson L. On the synovia in horses. Acta vet Scand 1971, 35:7-75.