The virulence of a Dickeya dadantii 3937 mutant devoid of osmoregulated periplasmic glucans is restored by inactivation of the RcsCD-RcsB phosphorelay

Journal of Bacteriology

Volumn 192, Issue 13, 2010, Pages 3484-3490

  Bouchart, Franck ^a   Boussemart, Gilles ^a   Prouvost, Anne France ^a   Cogez, Virginie ^a   Madec, Edwige ^a   Vidal, Olivier ^a   Delrue, Brigitte ^a   Bohin, Jean Pierre ^a   Lacroix, Jean Marie ^a  

^a UNIVERSITÉ DES SCIENCES ET TECHNOLOGIES DE LILLE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCAN; MEMBRANE PROTEIN; OSMOREGULATED PERIPLASMIC GLUCAN; PROTEIN RCSB; PROTEIN RCSC; PROTEIN RCSD; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL VIRULENCE; BACTERIUM MUTANT; CONTROLLED STUDY; DICKEYA DADANTII; ERWINIA; GENE INACTIVATION; NONHUMAN; PHENOTYPE; PLEIOTROPY; PRIORITY JOURNAL; PROTEIN FUNCTION;

BACTERIAL PROTEINS; CHICORY; ENTEROBACTERIACEAE; GENE EXPRESSION REGULATION, BACTERIAL; GLUCANS; MUTAGENESIS; PERIPLASM; SOLANUM TUBEROSUM; VIRULENCE; WATER-ELECTROLYTE BALANCE;

DICKEYA DADANTII; ENTEROBACTERIACEAE; PROTEOBACTERIA;

EID: 77954364645     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00143-10     Document Type: Article

References (41)

1. Adelberg, E. A., M. Mandel, and G. Chein Ching Chen. 1965. Optimal conditions for mutagenesis by N-methyl-N′-nitroso-N-nitrosoguanidine in Escherichia coli K12. Biochem. Biophys. Res. Commun. 18:788-795.
2. Andresen, L., V. Koiv, T. Alamae, and A. Mae. 2007. The Rcs phosphorelay modulates the expression of plant cell wall degrading enzymes and virulence in Pectobacterium carotovorum ssp. carotovorum. FEMS Microbiol. Lett. 273:229-238.
3. Bardonnet, N., and C. Blanco. 1992. ′uidA-antibiotic-resistance cassettes for insertion mutagenesis, gene fusions and genetic constructions. FEMS Microbiol. Lett. 72:243-247.
4. Bhagwat, A. A., W. Jun, L. Liu, P. Kannan, M. Dharne, B. Pheh, B. D. Tall, M. H. Kothary, K. C. Gross, S. Angle, J. Meng, and A. Smith. 2009. Osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium are required for optimal virulence in mice. Microbiology 155:229-237.
5. Bohin, J.-P., and J.-M. Lacroix. 2007. Osmoregulation in the periplasm, p. 325-341. In M. Ehrmann (ed.), The periplasm. ASM Press, Washington, DC.
6. Bouchart, F., A. Delangle, J. Lemoine, J.-P. Bohin, and J.-M. Lacroix. 2007. Proteomic analysis of a non virulent mutant of the phytopathogenic bacterium Erwinia chrysanthemi deficient in osmoregulated periplasmic glucans: change in protein expression is not restricted to the envelope, but affects general metabolism. Microbiology 153:760-767.
7. Bradford, M. M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
8. Carballès, F., C. Bertrand, J.-P. Bouché, and K. Cam. 1999. Regulation of Escherichia coli cell division genes ftsA and ftsZ by the two-component system rcsC-rcsB. Mol. Microbiol. 34:442-450.
9. Cherepanov, P. P., and W. Wackernagel. 1995. Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158:9-14.
10. Clavel, T., J.-C. Lazzaroni, A. Vianney, and R. Portalier. 1996. Expression of the tolQRA genes of Escherichia coli K12 is controlled by the RcsC sensor protein involved in capsule synthesis. Mol. Microbiol. 19:19-25.
11. Cogez, V., P. Talaga, J. Lemoine, and J.-P. Bohin. 2001. Osmoregulated periplasmic glucans of Erwinia chrysanthemi. J. Bacteriol. 183:3127-3133.
12. Delangle, A., A.-F. Prouvost, V. Cogez, J.-P. Bohin, J.-M. Lacroix, and N. Hugouvieux-Cotte-Pattat. 2007. Characterization of the Erwinia chrysanthemi gan locus, involved in galactan catabolism. J. Bacteriol. 189:7053-7061.
13. de Lorenzo, V., M. Herrero, U. Jakubzik, and K. N. Timmis. 1990. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J. Bacteriol. 172:6568-6572.
14. Dominguez-Bernal, G., M. G. Pucciarelli, F. Ramos-Morales, M. Garcia-Quintallina, D. A. Cano, J. Casadesus, and F. Garcia del Portillo. 2004. Repression of the RcsC-YojN-RcsB phosphorelay by the IgaA protein is a requisite for Salmonella virulence. Mol. Microbiol. 53:1437-1449.
15. Ebel, W., G. J. Vaughn, H. K. Peters III, and J. E. Trempy. 1997. Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli. J. Bacteriol. 179:6858-6861. (Pubitemid 27465326)
16. Erickson, K. D., and C. S. Detweiler. 2006. The Rcs phosphorelay system is specific to enteric pathogens/commensals and activates ydeI, a gene important for persistent Salmonella infection of mice. Mol. Microbiol. 62:883-894.
17. Fiedler, W., and H. Roterings. 1988. Properties of Escherichia coli mutants lacking membrane-derived oligosaccharides. J. Biol. Chem. 263:14684-14689.
18. Fineran, P. C., N. R. Williamson, K. S. Lilley, and G. P. C. Salmond. 2007. Virulence and prodigiosin antibiotic biosynthesis in Serratia are regulated pleiotropically by the GGDEF/EAL domain protein, PigX. J. Bacteriol. 189:7653-7662.
19. Francez-Charlot, A., B. Laugel, A. Van Gemert, N. Dubarry, F. Wiorowski, M. P. Castanié-Cornet, C. Gutierrez, and K. Cam. 2003. RcsCDB His-Asp phosphorelay system negatively regulates the flhDC operon in Escherichia coli. Mol. Microbiol. 49:823-832.
20. García-Calderon, C. B., M. García-Quintanilla, J. Casadesus, and F. Ramos-Morales. 2005. Virulence attenuation in Salmonella enterica rcsC mutants with constitutive activation of the Rcs system. Microbiology 151:579-588.
21. Herrero, M., V. De Lorenzo, and K. N. Timmis. 1990. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J. Bacteriol. 172:6557-6567. (Pubitemid 20372801)
22. Hugouvieux-Cotte-Pattat, N., G. Condemine, W. Nasser, and S. Reverchon. 1996. Regulation of pectinolysis in Erwinia chrysanthemi. Annu. Rev. Microbiol. 50:213-257.
23. Huguet, E., K. Hahn, K. Wengelnik, and U. Bonas. 1998. hpaA mutants of Xanthomonas campestris pv. vesicatoria are affected in pathogenicity but retain the ability to induce host-specific hypersensitive reaction. Mol. Microbiol. 29:1379-1390.
24. Lojkowska, E., C. Masclaux, M. Boccara, J. Robert-Baudouy, and N. Hugouvieux-Cotte-Pattat. 1995. Characterization of the pelL gene encoding a novel pectate lyase of Erwinia chrysanthemi 3937. Mol. Microbiol. 16:1183-1195.
25. Loubens, I., L. Debarbieux, A. Bohin, J.-M. Lacroix, and J.-P. Bohin. 1993. Homology between a genetic locus (mdoA) involved in the osmoregulated biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus (hrpM) controlling pathogenicity of Pseudomonas syringae. Mol. Microbiol. 10:329-340.
26. Mahajan-Miklos, S., M.-W. Tan, L. G. Rahme, and F. M. Ausubel. 1999. Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa-Caenorhabditis elegans pathogenesis model. Cell 96:47-56.
27. Majdalani, N., and S. Gottesman. 2005. The Rcs phosphorelay: a complex signal transduction system. Annu. Rev. Microbiol. 59:379-405.
28. Mariscotti, J. F., and F. García-del Portillo. 2009. Genome expression analyses revealing the modulation of the Salmonella Rcs regulon by the attenuator IgaA. J. Bacteriol. 191:1855-1867.
29. Miller, J. H. 1992. A short course in bacterial genetics: a laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
30. Miller, V. L., and J. J. Mekalanos. 1988. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170:2575-2583.
31. Mouslim, C., M. Delgado, and E. A. Groisman. 2004. Activation of the RcsC/YojN/RcsB phosphorelay system attenuates Salmonella virulence. Mol. Microbiol. 54:386-395.
32. Page, F., S. Altabe, N. Hugouvieux-Cotte-Pattat, J.-M. Lacroix, J. Robert-Baudouy, and J.-P. Bohin. 2001. Osmoregulated periplasmic glucan synthesis is required for Erwinia chrysanthemi pathogenicity. J. Bacteriol. 183:3134-3141.
33. Perombelon, M., and A. Kelman. 1980. Ecology of the soft rot Erwinia. Annu. Rev. Phytopathol. 18:361-387.
34. Resibois, A., M. Colet, M. Faelen, T. Schoonejans, and A. Toussaint. 1984. Phi-EC2, a new generalized transducing phage of Erwinia chrysanthemi. Virology 137:102-112.
35. Roeder, D. L., and A. Collmer. 1985. Marker-exchange mutagenesis of a pectate lyase isozyme gene in Erwinia chrysanthemi. J. Bacteriol. 164:51-56.
36. Sambrook, J. E., F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
37. Simon, R., J. Quandt, and W. Klipp. 1989. New derivatives of transposon Tn5 suitable for mobilization of replicons, generation of operons fusions and induction of genes in Gram-negative bacteria. Gene 80:161-169.
38. Tomenius, H., A.-K. Pernestig, C. F. Mendez-Catala, D. Georgellis, S. Normark, and O. Melefors. 2005. Genetic and functional characterization of the Escherichia coli BarA-UvrY two-component system: point mutations in the HAMP linker of the BarA sensor give a dominant-negative phenotype. J. Bacteriol. 187:7317-7324.
39. Wu, T. T. 1966. A model for a three point analysis of random general transduction. Genetics 54:405-410.
40. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103-119.
41. Zhou, L., X. H. Lei, B. R. Bochner, and B. L. Wanner. 2003. Phenotype microarray analysis of Escherichia coli K-12 mutants with deletions of all two-component systems. J. Bacteriol. 185:4956-4972.