Molecular cloning and expression profiling of a chalcone synthase gene from hairy root cultures of Scutellaria viscidula Bunge

Genetics and Molecular Biology

Volumn 33, Issue 2, 2010, Pages 285-291

  Lei, Wei ^a   Tang, Shao Hu ^a   Luo, Ke Ming ^a   Sun, Min ^a  

^a SOUTHWEST UNIVERSITY   (China)

Author keywords

Chalcone synthase gene; Methyl jasmonate; Molecular cloning; Scutellaria viscidula Bunge

Indexed keywords

CHALCONE SYNTHASE; COMPLEMENTARY DNA; JASMONIC ACID METHYL ESTER; MALONYL COENZYME A;

ALFALFA; ALPHA HELIX; AMINO ACID SEQUENCE; ARTICLE; CYCLIZATION; ENZYME ACTIVE SITE; GENE EXPRESSION PROFILING; HAIRY ROOT CULTURE; HYDROPHILICITY; ISOELECTRIC POINT; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHYLOGENY; PHYTOCHEMISTRY; PROTEIN BINDING; PROTEIN MOTIF; PROTEIN SECONDARY STRUCTURE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCUTELLARIA; SCUTELLARIA BAICALENSIS; SCUTELLARIA VISCIDULA; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

MEDICAGO SATIVA; SCUTELLARIA BAICALENSIS; SCUTELLARIA VISCIDULA;

EID: 77954361819     PISSN: 14154757     EISSN: 16784685     Source Type: Journal    
DOI: 10.1590/S1415-47572010005000031     Document Type: Article

References (35)

1. Arnold K, Bordoli L, Kopp J and Schwede T (2006) The Swiss- Model Workspace: A web-based environment for protein structure homology modeling. Bioinformatics 22:195-201.
2. Assia B, Nadège T, Cassandrine S, Stéphanie C, Françis B, Saïd H and Mérillon JM (2008) Effect of methyl jasmonate in combination with carbohydrates on gene expression of PR proteins, stilbene and anthocyanin accumulation in grapevine cell cultures. Plant Physiol Biochem 46:493-499.
3. Combet C, Blanchet C, Geourjon C and Deléage G (2000) NPS@: Network Protein Sequence Analysis. Trends Biochem Sci 3:147-150.
4. Creelman R and Mullet JE (1997) Biosynthesis and action of jasmonates in plants. Annu Rev Plant Phys 48:355-381.
5. Ferrer JL, Jez JM, Bowman ME, Dixon RA and Noel JP (1999) Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis. Nature 8:775-840.
6. Heidrun G, Martin JM, Toni MK, Kuchan TM and Meinhart HZ (1992) Jasmonic acid is a signal transducer in elicitorinduced plant cell cultures. Proc Natl Acad Sci USA 89:2389-2393.
7. Hrazdina G (1992) Compartmentation in aromatic metabolism. In: Stafford HA and Ibrahim RK (eds) Recent Advances in Phytochemistry. Plenum Press, New York, pp 1-23.
8. Ikeda M, Arai M and Lao DM (2002) Transmembrane topology prediction methods: A reassessment and improvement by a consensus method using a dataset of experimentally characterized transmembrane topologies. In Silico Biol 2:1-33.
9. Ikemoto S, Sugimura K, Yoshida N, Yasumoto R, Wada S, Yamamoto K and Kishimoto T (2000) Antitumor effects of Scutellariae radix and its components baicalein, baicalin, and wogonin on bladder cancer cell lines. J Urol 55:951- 955.
10. Jensen JL, Gupta R, Blom N, Devos D, Tamames J, Kesmir C, Nielsen H, Staerfeldt H, Rapacki K and Workman C (2002) Prediction of human orphan protein function from posttranslational modifications and localization features. J Mol Biol 319:1257-1265.
11. Jensen JL, Stærfeldt HH and Brunak S (2003) Prediction of human protein function according to gene ontology categories. Bioinformatics 19:635-642.
12. Jez JM and Noel JP (2000) Mechanism of chalcone synthase: pKa of the catalytic cysteine and the role of the conserved histidine in a plant polyketide synthase. Biol Chem 275:39640- 39643.
13. Jiang M and Cao JS (2007) Chalcone synthase gene. Chinese J Cell Biol 29:525-529.
14. Kovács G, Kuzovkina IN, Szõke É and Kursinszki L (2004) HPLC determination of flavonoids in hairy-root cultures of Scutellaria baicalensis Georgi. Chromatographia 60:S81- S85.
15. Kristin E and Siegfried H (2004) InvB is required for type IIIdependent secretion of sopA in Salmonella enterica serovar typhimurium. J Bacteriol 186:1215-1219.
16. Kumar SK, Tmamura K, Jakobseni IB and Nei M (2001) MEGA2: Molecular Evolutionary Genetics Analysis software. Bioinformatics 17:1244-1245.
17. Kyte J and Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105- 132.
18. Lei W, Sun M, Luo KM, Shui XR, Sun YM and Tang SH (2009) Computer simulations to characterize chalcone synthase from Scutellaria baicalensis Georgi. Mol Biol 43:1012- 1017.
19. Murashige T and Skoog K (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15:473-479.
20. Sánchez-Sampedro MA, Fernández-Tárrago J and Corchete P (2005) Yeast extract and methyl jasmonate-induced silymarin production in cell cultures of Silybum marianum (L.) Gaertn. J Biotechnol 119:60-69.
21. Shieh DE, Liu LT and Lin CC (2000) Antioxidant and free radical scavenging effects of baicalein, baicalin and wogonin. Anticancer Res 20:2861-2865.
22. So FV, Guthrie N, Chambers AF and Carroll KK (1997) Inhibition of proliferation of estrogen receptor-positive MCF-7 human breast cancer cells by flavonoids in the presence and absence of excess estrogen. Cancer Lett 112:127-133.
23. Stefan M and Axel M (2005) Flavones and flavone synthases. Phytochemistry 66:2399-2407.
24. Thompson JD, Gibson TJ, Plewniaki F, Jeanmougin F and Higgins DG (1997) The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876-4882.
25. Wang SF (2007) Establishment of transform system in Scutellaria viscidula Bunge and its flavonoids production. Master's Thesis, Southwest University, Chongqing.
26. Wang SF, Sun YM, Lei W, Yang R and Sun M (2008a) Induction and culture of hairy roots in Scutellaria viscidula and its baicalin production. China J Chin Mater Med 33:1669-1672.
27. Wang SY, Bowman L and Ding M(2008b) Methyl jasmonate enhances antioxidant activity and flavonoid content in blackberries (Rubus sp.) and promotes antiproliferation of human cancer cells. Food Chem 107:1261-1269.
28. Winkel SB (2002) Biosynthesis of flavonoids and effects of stress. Curr Opin Plant Biol 5:218-223.
29. Yamamoto H (1991) Biotechnology in agriculture and forestry. In: Bajaj YPS (ed) Medicinal and Aromatic Plants III. Springer Press, Berlin, pp 398-418.
30. Ye F, Xui L, Yi J, Zhang W and Zhang DY (2002) Anticancer activity of Scutellaria baicalensis and its potential mechanism. J Altern Complem Med 8:567-572.
31. Yukihito Y, Homare T, Yosuke H and Yasuhiro H (1996) Methyl jasmonate-induced overproduction of paclitaxel and baccatin III in Taxus cell suspension cultures. Nat Biotechnol 14:1129-1132.
32. Zhang JJ and Xu MJ (2006) Effects of nitric oxide and methyl jasmonate on the baicalin production and cell growth in suspension cultures of Scutellaria baicalensis. Chinese Bull Bot 23:374-379.
33. ORF Finder, http://www.ncbi.nlm.nih.gov/gorf/gorf.html.
34. BLASTP program, http://www.ncbi.nih.gov/BLAST.
35. SWISS-MODEL, http://swissmodel.expasy.org.