Explant outgrowth, propagation and characterization of human pericytes

Microcirculation

Volumn 17, Issue 5, 2010, Pages 367-380

  Maier, Cheryl L ^a   Shepherd, Benjamin R ^a,b   Yi, Tai ^a   Pober, Jordan S ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Organovo Inc   (United States)

Author keywords

endothelial cell; microvessel; pericyte isolation; placenta

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; BINDING PROTEIN; CD146 ANTIGEN; PLATELET DERIVED GROWTH FACTOR BETA RECEPTOR; PROTEIN NG2; THY 1 ANTIGEN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BLOOD VESSEL PERMEABILITY; CELL CULTURE; CELL POPULATION; CELL TYPE; ENDOTHELIUM CELL; EXPLANT; FEMALE; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IMPLANTATION; LEUKOCYTE; MESENCHYME CELL; MICROVASCULATURE; MOUSE; NONHUMAN; PERICYTE; PLACENTA; PRIORITY JOURNAL; SMOOTH MUSCLE FIBER; STROMA CELL;

ANIMALS; BIOLOGICAL MARKERS; CAPILLARY PERMEABILITY; CELL CULTURE TECHNIQUES; CELL SEPARATION; ENDOTHELIAL CELLS; FEMALE; GENE EXPRESSION PROFILING; HUMANS; IMMUNOPHENOTYPING; MICE; MICE, SCID; MICROVESSELS; MYOCYTES, SMOOTH MUSCLE; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PERICYTES; PLACENTA; PREGNANCY; TRANSPLANTATION, HETEROLOGOUS;

MUS;

EID: 77954344357     PISSN: 10739688     EISSN: 15498719     Source Type: Journal    
DOI: 10.1111/j.1549-8719.2010.00038.x     Document Type: Article

References (15)

1. Bryan BA, D'Amore PA. Pericyte isolation and use in endothelial/pericyte coculture models. Meth Enzymol 443 : 315 331, 2008.
2. Churchman AT, Siow RCM. Isolation, culture, and characterization of vascular smooth muscle cells. Methods Mol Biol 467 : 127 138, 2009.
3. Crisan M, Yap S, Casteilla L, Chen CW, Corselli M, Park TS, Andriolo G, Sun B, Zheng B, Zhang L, Norotte C, Teng PN, Traas J, Schugar R, Deasy BM, Badylak S, Buhring HJ, Giacobino JP, Lazzari L, Huard J, Peault B. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell 3 : 301 313, 2008.
4. Greenberg JI, Shields DJ, Barillas SG, Acevedo LM, Murphy E, Huang J, Scheppke L, Stockmann C, Johnson RS, Angle N, Cheresh DA. A role for VEGF as a negative regulator of pericyte function and vessel maturation. Nature 456 : 809 813, 2008.
5. Hammes HP. Pericytes and the pathogenesis of diabetic retinopathy. Horm Metab Res 37 : 39 43, 2005.
6. Hirschi KK, D'Amore PA. Pericytes in the microvasculature. Cardiovasc Res 32 : 687 698, 1996.
7. Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 52 : 2745 2756, 1973.
8. Jain RK. Normalizing tumor vasculature with anti-angiogenic therapy: a new paradigm for combination therapy. Nat Med 7 : 987 989, 2001.
9. Johnson PC, Mikos AG, Fisher JP, Jansen JA. Strategic directions in tissue engineering. Tissue Eng 13 : 2827 2837, 2007.
10. Lindahl P, Johansson BR, Leveen P, Betsholtz C. Pericyte loss and microaneurysm formation in PDGF-B-deficient mice. Science 277 : 242 245, 1997.
11. Proudfoot D, Skepper JN, Shanahan CM, Weissberg PL. Calcification of human vascular cells in vitro is correlated with high levels of matrix Gla protein and low levels of Osteopontin expression. Arterioscler Thomb Vasc Biol 18 : 379 388, 1998.
12. Shepherd BR, Jay SM, Saltzman WM, Tellides G, Pober JS. Human aortic smooth muscle cells promote arteriole formation by coengrafted endothelial cells. Tissue Eng Part A 15 : 165 173, 2009.
13. Song L, Webb NE, Song Y, Tuan RS. Identification and functional analysis of candidate genes regulating mesenchymal stem cell self-renewal and multipotency. Stem Cells 24 : 1707 1718, 2006.
14. Sundberg C, Kowanetz M, Brown LF, Detmar M, Dvorak HF. Stable expression of angiopoietin-1 and other markers by cultured pericytes: phenotypic similarities to a subpopulation of cells in maturing vessels during later stages of angiogenesis in vivo. Lab Invest 82 : 387 401, 2002.
15. Thornton SC, Mueller SN, Levine EM. Human endothelial cells: use of heparin in cloning and long-term serial cultivation. Science 222 : 623 625, 1983.