Hyperexpression of two Aspergillus niger xylanase genes in Escherichia coli and characterization of the gene products

Brazilian Journal of Microbiology

Volumn 41, Issue 3, 2010, Pages 778-786

  Yi, Xiuli ^a,b   Shi, Yan ^a,b   Xu, Hui ^c   Li, Wei ^a,b   Xie, Jie ^a,b   Yu, Rongqing ^a,b   Zhu, Jun ^a,b   Cao, Yi ^a,b   Qiao, Dairong ^a,b  

^a SICHUAN UNIVERSITY   (China)

^b Microbiology and Metabolic Engineering key Laboratory of Sichuan Province   (China)

^c Sichuan BioShine Biotechnology Co Ltd   (China)

Author keywords

Aspergillus niger; Enzymatic characterization; Prokaryotic expression; Xylanase

Indexed keywords

ASPERGILLUS NIGER; ESCHERICHIA COLI; PROKARYOTA;

EID: 77954303370     PISSN: 15178382     EISSN: 16784405     Source Type: Journal    
DOI: 10.1590/S1517-83822010000300030     Document Type: Article

References (30)

1. Asano, K.; Sriprang, R.; Gobsuk, J.; Eurwilaichitr, L.; Tanapongpipat, S.; Kirtikara, K. (2005). Endo-1,4-β-xylanase B from Aspergillus niger BCC14405 Isolated in Thailand: Purification, Characterization and Gene Isolation. J. Biochem. Mol. Biol., 38, 17-23.
2. Berrin, J.G.; Williamson, G.; Puigserver, A.; Chaix, J.C.; McLauchlan, W.R.; Juge, N. (2000). High-level production of recombinant fungal endo-beta-1,4-xylanase in the methylotrophic yeast Pichia pastoris. Protein Expr. Purif., 19, 179-187.
3. Bradford, M.M. (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254.
4. Bruins, M.E.; Jasen, A.E.; Boom, R.M. (2001). Thermozymes and their applications: a review of recent literature and patents. Appl. Biochem. Biotechnol., 90, 155-186.
5. Chen, A.P.C.; Chang, S.; Lin, Y.; Sun, Y.; Chen, C.; Wang, A.H.J.; Liang, P. (2005). Substrate and product specificities of cistype undecaprenyl pyrophosphate synthase. Biochem. J., 386, 169-176.
6. Coughlan, M.P.; Hazlewood, G.P. (1993). B-1,4-d-Xylan-degrading enzyme systems:biochemistry, molecular biology and applications. Biotechnol. Appl. Biochem., 17, 259-289.
7. Dominguez, R.; Souchon, H.; Spinelli, S.; Dauter, Z.; Wilson, K.S.; Chauvaux, S.; Beguin, P.; Alzari, P.M. (1995). A common protein fold and similar active site in two distinct families of β-glycanases. Nat. Struct. Biol., 2, 569-576.
8. Frederick, M.M,; Kiang, C.; Frederick, J.R.; Reilly, P.J. (1985). Purification and characterization of endo-xylanases from Aspergillus niger. 1. Two isozymes active on xylan backbones near branch points. Biotechnol. Bioeng., 27, 525-532.
9. Gilkes, N.R.; Henrissat, B.; Kilburn, D.G.; Miller, R.C.; Warren, R.A.J. (1991). Domains in microbial β-1,4-glycanases: sequence conservation, function,and enzyme families. Microbiol. Rev., 55, 303-315.
10. Gorbacheva, I.V.; Rodionova, N.A. (1977). Studies on xylan degrading enzymes. I. Purification and characterization of endo-1,4-beta-xylanase from Aspergillus niger str. 14. Biochim.Biophys. Acta., 484, 79-93.
11. Haki, G.D; Rakshit, S.K. (2003). Developments in industrially important thermostable enzymes: a review. Bioresour. Technol., 89, 17-34
12. Henrissat, B.; Bairoch, A. (1993). New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J., 293, 781-788.
13. Kinoshita, K.; Takano, M.; Koseki, T.; Ito, K.; Iwano, K. (1995). Cloning of the xynNB gene encoding xylanase B from Aspergillus niger and its expression in Aspergillus kawachii. J.Ferment. Bioeng., 79, 422-428.
14. Korona, B.; Korona, D.; Bielecki, S. (2006). Efficient expression and secretion of two co-produced xylanases from Aspergillus niger in Pichia pastoris directed by their native signal peptides and the Saccharomyces cerevisiae α-mating factor. Enzyme Microb. Technol., 39, 683-689.
15. Krengel, U.; Dijkstra, B.W. (1996). Three-dimensional structure of endo-1, 4-xylanase I from Aspergillus niger: molecular basis for its low pH optimum. J. Mol. Biol., 263, 70-78.
16. Kulkarni, N.; Shendye, A.; Rao, M. (1999). Molecular and biotechnological aspects of xylanases. FEMS. Microbiol. Rev., 23, 411-456.
17. Laemmli, U.K. (1970). Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
18. Liu, L.; Li, X.; Li, X.; Shao, W. (2004). Computational analysis of responsible dipeptides for optimum pH in G/11 xylanase. Biochem. Biophys. Res. Commun., 321, 391-396.
19. Liu, M.Q.; Weng, X.Y.; Sun, J.Y. (2006). Expression of recombinant Aspergillus niger xylanase A in Pichia pastoris and its action on xylan. Protein Expr. Purif., 48, 292-299.
20. Luttig, M.; Pretorius, I.S.; Van-Zyl, W.H. (1997). Cloning of two β-xylanase encoding genes from Aspergillus niger and their expression in Saccharomyces cerevisiae. Biotechnol. Lett., 19, 411-415.
21. Miller, G.L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31, 426-428.
22. Rogalski, J.; Oleszek, M.; Tokarzewska-Zadora, J. (2001). Purification and characterization of two endo-1,4 beta-xylanase and a 3-xylosidase from Phlebia adiate. Acta. Microbiol. Pol., 50, 117-128.
23. Saiki, R.K.; Gelvand, D.H.; Stoffel, S.; Scharf, S.J.; Higuchi, R.; Horn, G.T.; Mullis, K.B.; Erlich, H.A. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239, 487-491.
24. Singh, S.; Madlala, A.M.; Prior, B.A. (2003). Thermomyces lanuginosus: properties of strains and their hemicellulases. FEMS. Microbiol. Rev., 27, 3-16.
25. Subramaniyan, S.; Prema, P. (2002). Biotechnology of microbial xylanases: enzymology. Crit. Rev. Biotechnol., 22, 33-64
26. Sunna, A.; Antranikian, G. (1997). Xylanolytic enzymes from fungi and bacteria. Crit. Rev. Biotechnol., 17, 39-67.
27. Tahir, T.A.; Berrin, J.G.; Flatman, R.; Roussel, A.; Roepstorff, P.; Williamson, G.; Juge, N. (2002). Specific characterization of substrate and inhibitor binding sites of a glycosyl hydrolase family 11 xylanase from Aspergillus niger. J. Biol. Chem., 277, 44035-44043.
28. Tahir, T.A.; Durand, A.; Gebruers, K.; Roussel, A.; Williamson, G.; Juge, N. (2004). Functional importance of Asp37 from a family 11 xylanase in thebinding to two proteinaceous xylanase inhibitors from wheat. FEMS. Microbiol. Lett., 239, 9-15.
29. Torronen, A.; Harkki, A.; Rouvinen, J. (1994). Three-dimensional structure of endo-1,4-β-xylanase II from Trichoderma reesei: two conformational states in the active site. EMBO. J., 13, 2493-2501.
30. Wong, K.K.Y.; Tan, L.U.L.; Saddler, J.N. (1988). Multiplicity of β-1,4-xylanase in microorganisms: function and applicants. Microbiol. Rev., 52, 305-317.