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Cold Spring Harbor Protocols
Volumn 3, Issue 10, 2008, Pages
qRT-PCR of microbial biofilms
Author keywords

[No Author keywords available]
Indexed keywords

EID: 77954288384     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5066     Document Type: Article
Times cited : (8)
References (11)
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  • 2
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  • 3
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    • Pérez-Osorio A.C., Franklin M.J. 2008. Isolation of RNA and DNA from biofilm samples obtained by laser capture microdissection microscopy. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb. prot5065.[Abstract/Free Full Text]
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    • Pérez-Osorio, A.C.1    Franklin, M.J.2
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    • Analysis of relative gene expression data using real-time quantitative PCR and the 2-CT method
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    • Livak K.J., Schmittgen T.D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-CT method. Methods 25: 402-408.[Medline]
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    • Sensitive quantitative detection of commensal bacteria by rRNA-targeted reverse transcription (RT)-PCR
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    • Matsuda K., Tsuji H., Asahara T., Kado Y., Nomoto K. 2007. Sensitive quantitative detection of commensal bacteria by rRNA-targeted reverse transcription (RT)-PCR. Appl. Environ. Microbiol. 73: 32-39.[Abstract/Free Full Text]
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    • Matsuda, K.1    Tsuji, H.2    Asahara, T.3    Kado, Y.4    Nomoto, K.5
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    • Quantification of mRNA using real-time RT-PCR
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    • Nolan T., Hands R.E., Bustin S.A. 2006. Quantification of mRNA using real-time RT-PCR. Nat. Protoc. 1: 1559-1582.[Medline]
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    • Nolan, T.1    Hands, R.E.2    Bustin, S.A.3
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    • Novel methods of quantitative real-time PCR data analysis in a murine Helicobacter pylori vaccine model
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    • Rozen S., Skaletsky H. 2000. Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 132: 365-386.[Medline]
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    • Agarose gel electrophoresis
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