Fixation methods for electron microscopy of human and other liver

World Journal of Gastroenterology

Volumn 16, Issue 23, 2010, Pages 2851-2866

  Wisse, Eddie ^a,b   Braet, Filip ^b   Duimel, Hans ^a   Vreuls, Celien ^a   Koek, Ger ^a   Olde Damink, Steven W M ^a   van den Broek, Maartje A J ^a   de Geest, Bart ^c   Dejong, Cees H C ^a   Tateno, Chise ^d   Frederik, Peter ^a  

^a MAASTRICHT UNIVERSITY   (Netherlands)

^b UNIVERSITY OF SYDNEY   (Australia)

^c UNIVERSITY OF LEUVEN   (Belgium)

^d PhoenixBio   (Japan)

Author keywords

Biopsy; Electron microscopy; Liver; Perfusion; Tissue fixation

Indexed keywords

ANALGESIC AGENT; ANESTHETIC AGENT; CHLORAL HYDRATE; FORMALDEHYDE; GLUTARALDEHYDE; PENTOBARBITAL; PHOSPHATE BUFFERED SALINE; TOLUIDINE DERIVATIVE;

ANESTHESIA; ARTICLE; CANNULATION; CRYOELECTRON MICROSCOPY; CRYOPRECIPITATION; DRUG OVERDOSE; ELECTRON MICROSCOPY; EMBEDDING; HISTOPATHOLOGY; HUMAN; IMMUNOHISTOCHEMISTRY; LIVER; LIVER BIOPSY; LIVER CELL; LIVER CELL CULTURE; LIVER DISEASE; LIVER METABOLISM; LIVER PARENCHYMA; LIVER PERFUSION; MICROSCOPY; MINIPIG; NEEDLE; NONHUMAN; PHYSICIAN; REAL TIME ECHOGRAPHY; SCANNING ELECTRON MICROSCOPY; TISSUE FIXATION; TRANSMISSION ELECTRON MICROSCOPY; ANIMAL; BIOPSY; CELL CULTURE; HISTOLOGY; PERFUSION; PROCEDURES; ULTRASTRUCTURE;

ANIMALS; BIOPSY; CELLS, CULTURED; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; HUMANS; LIVER; MICROSCOPY, ELECTRON; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, ELECTRON, TRANSMISSION; PERFUSION; TISSUE FIXATION;

EID: 77954279032     PISSN: 10079327     EISSN: None     Source Type: Journal    
DOI: 10.3748/wjg.v16.i23.2851     Document Type: Article

References (38)

1. Geuze HJ. A future for electron microscopy in cell biology? Trends Cell Biol 1999; 9: 92-93
2. Braet F, Ratinac K. Creating next-generation microscopists: structural and molecular biology at the crossroads. J Cell Mol Med 2007; 11: 759-763
3. Rockey DC, Caldwell SH, Goodman ZD, Nelson RC, Smith AD. Liver biopsy. Hepatology 2009; 49: 1017-1044
4. Claude A, Fullam EF. The preparation of sections of guinea pig liver for electron microscopy. J Exp Med 1946; 83: 499-503
5. Fahimi HD. Perfusion and immersion fixation of rat liver with glutaraldehyde. Lab Invest 1967; 16: 736-750
6. Wisse E. An electron microscopic study of the fenestrated endothelial lining of rat liver sinusoids. J Ultrastruct Res 1970; 31: 125-150
7. Bhatnagar MK, David LL, Vrablic O, Therien A, Blouin A. A simple method for perfusion fixation of avian liver for electron microscopy. Can J Zool 1981; 59: 1179-1183
8. Balabaud C, Boulard A, Quinton A, Saric J, Bedin C, Boussarie L, Bioulac-Sage P. Light and transmission electron microscopy of sinusoids in human liver. In: Bioulac-Sage P, Balabaud C, editors. Sinusoids in human liver: health and disease. Rijswijk: The Kupffer Cell Foundation, 1988: 87-110
9. Burwen SJ, Jones AL, Goldman IS, Way LW, Dejbakhsh S. The perfused human liver wedge biopsy: a new in vitro model for morphological and functional studies. Hepatology 1982; 2: 426-432
10. Muto M, Nishi M, Fujita T. Scanning electron microscopy of human liver sinusoids. Arch Histol Jpn 1977; 40: 137-151
11. Sandström B. Liver fixation for electron microscopy by means of transparenchymal perfusion with glutaraldehyde. Lab Invest 1970; 23: 71-73
12. Vonnahme FJ. An improved method for transparenchymal fixation of human liver biopsies for scanning electron microscopy. Scan Electron Microsc 1980; 177-180
13. Wisse E, Jacobs F, Topal B, Frederik P, De Geest B. The size of endothelial fenestrae in human liver sinusoids: implications for hepatocyte-directed gene transfer. Gene Ther 2008; 15: 1193-1199
14. Bioulac-Sage P, Lamouliatte H, Saric J, Balabaud C. [Perfusion- fixation of liver needle biopsy: technic] Gastroenterol Clin Biol 1984; 8: 656-659
15. De Wilde A, Van Der Spek P, Devis G, Wisse E. On the fixation of needle biopsies of rat liver tissue as a model to study the fine structure of sinusoidal cells. In: Knook DL, Wisse E, editors. Sinusoidal Liver Cells. Rijswijk: The Kupffer Cell Foundation, 1982: 85-92
16. Horn T, Christoffersen P. Perfusion fixation of hepatic needle biopsies for scanning electron microscopy. A methodological study. Liver 1986; 6: 89-97
17. Gendrault JL, Montecino-Rodriguez F, Cinqualbre J. Structure of the normal human liver sinusoid after perfusion fixation. In: Knook DL, Wisse E, editors. Sinusoidal Liver Cells. Amsterdam: Elsevier/North-Holland Biomedical Press, 1982: 93-100
18. Wisse E, De Wilde A, De Zanger R. Perfusion fixation of human and rat liver tissue for light and electron microscopy: a review and assessment of existing methods with special emphasis on sinusoidal cells and microcirculation. In: O'Hare AMF, editor. Science of Biological Specimen Preparation. Chicago: SEM Inc., 1984: 31-38
19. Bullock GR. The currejnt status of fixation for electron microscopy: a review. J Microscopy 1984; 133: 1-15
20. White DL, Mazurkiewicz JE, Barrnett RJ. A chemical mechanism for tissue staining by osmium tetroxide-ferrocyanide mixtures. J Histochem Cytochem 1979; 27: 1084-1091
21. Hayat MA. Principles and techniques of electron microscopy: biological applications. Cambridge: Cambridge University Press, 2000
22. Wisse E, De Zanger RB, Jacobs R, McCuskey RS. Scanning electron microscope observations on the structure of portal veins, sinusoids and central veins in rat liver. Scan Electron Microsc 1983; 1441-1452
23. Wisse E, De Zanger RB, Charels K, Van Der Smissen P, Mc- Cuskey RS. The liver sieve: considerations concerning the structure and function of endothelial fenestrae, the sinusoidal wall and the space of Disse. Hepatology 1985; 5: 683-692
24. Boyde A, Wood C. Preparation of animal tissues for surfacescanning electron microscopy. J Microsc 1969; 90: 221-249
25. Nation JL. A new method using hexamethyldisilazane for preparation of soft insect tissues for scanning electron microscopy. Stain Technol 1983; 58: 347-351
26. Boyde A. Review of basic preparation techniques for biological scanning electron microscopy. In: Brederoo P, de Priester W, editors. Electron microscopy. The Hague: 7th Eur. Congress Electron Microsc Foundation Leiden, 1980: 768-777
27. Braet F, De Zanger R, Wisse E. Drying cells for SEM, AFM and TEM by hexamethyldisilazane: a study on hepatic endothelial cells. J Microsc 1997; 186: 84-87
28. Frenzel H, Kremer B, Richter IE, Hücker H. [The fine structure of liver sinusoids after perfusion fixation with various pressures. A transmission and scanning electron microscopic study (author's transl)] Res Exp Med (Berl) 1976; 168: 229-241
29. Fox CH, Johnson FB, Whiting J, Roller PP. Formaldehyde fixation. J Histochem Cytochem 1985; 33: 845-853
30. Davies B, Morris T. Physiological parameters in laboratory animals and humans. Pharm Res 1993; 10: 1093-1095
31. DeLeve LD. Hepatic microvasculature in liver injury. Semin Liver Dis 2007; 27: 390-400
32. Tanuma Y, Ohata M, Ito T. A transmission electron microscopic study of rabbit liver sinusoids with special remarks on an experimentally induced canalicular system and the "pored domes" in the endothelial cells. Arch Histol Jpn 1987; 50: 177-192
33. Braet F, Muller M, Vekemans K, Wisse E, Le Couteur DG. Antimycin A-induced defenestration in rat hepatic sinusoidal endothelial cells. Hepatology 2003; 38: 394-402
34. David H, Uerlings I. Quantitative ultrastructure of the rat liver by immersion and perfusion fixations. Exp Pathol 1983; 23: 131-141
35. Lievens J, Snoeys J, Vekemans K, Van Linthout S, de Zanger R, Collen D, Wisse E, De Geest B. The size of sinusoidal fenestrae is a critical determinant of hepatocyte transduction after adenoviral gene transfer. Gene Ther 2004; 11: 1523-1531
36. Snoeys J, Lievens J, Wisse E, Jacobs F, Duimel H, Collen D, Frederik P, De Geest B. Species differences in transgene DNA uptake in hepatocytes after adenoviral transfer correlate with the size of endothelial fenestrae. Gene Ther 2007; 14: 604-612
37. Arborgh B, Bell P, Brunk U, Collins VP. The osmotic effect of glutaraldehyde during fixation. A transmission electron microscopy, scanning electron microscopy and cytochemical study. J Ultrastruct Res 1976; 56: 339-350
38. Desmet VJ. The amazing universe of hepatic microstructure. Hepatology 2009; 50: 333-344