Genomic cloning, characterization and statistical analysis of an antitumor-analgesic peptide from Chinese scorpion Buthus martensii Karsch

Toxicon

Volumn 56, Issue 3, 2010, Pages 432-439

  Cui, Yong ^a   Liu, Yanfeng ^a   Chen, Qiqing ^a   Zhang, Rong ^a   Song, Yongbo ^a   Jiang, Zhuopu ^a   Wu, Chunfu ^a   Zhang, Jinghai ^a  

^a SHENYANG PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Antitumor analgesic peptide; Genomic organization; Intron splicing; Statistical analysis; Transcription regulatory factor

Indexed keywords

ADENINE; ANTITUMOR ANALGESIC PEPTIDE; COMPLEMENTARY DNA; GENOMIC DNA; GLYCINE; GUANINE; LIGAND; OLIGONUCLEOTIDE; SCORPION VENOM; SODIUM CHANNEL; THYMINE; UNCLASSIFIED DRUG;

ARTICLE; CODON; COMPUTER ANALYSIS; CONTROLLED STUDY; DNA EXTRACTION; DNA SEQUENCE; GENE AMPLIFICATION; GENE EXPRESSION; GENE SEQUENCE; GENETIC ANALYSIS; INTRON; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN FUNCTION; SCORPION; STATISTICAL ANALYSIS;

ANALGESICS; ANIMALS; ANTINEOPLASTIC AGENTS; CLONING, MOLECULAR; GENOMICS; SCORPIONS;

MESOBUTHUS MARTENSII; SCORPIONES;

EID: 77954143055     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2010.04.012     Document Type: Article

References (36)

1. Alami M., Ceard B., Legros C., Bougis P.E., Martin-Eauclaire M.F. Genomic characterisation of the toxin Amm VIII from the scorpion Androctonus mauretanicus mauretanicus. Toxicon 2006, 47(5):531-536.
2. Becerril B., Corona M., Coronas F.I., Zamudio F., Calderon-Aranda E.S., Fletcher P.L., Martin B.M., Possani L.D. Toxic peptides and genes encoding toxin gamma of the Brazilian scorpions Tityus bahiensis and Tityus stigmurus. Biochem. J. 1996, 313(PT 3):753-760.
3. Becerril B., Corona M., Mejia M.C., Martin B.M., Lucas S., Bolivar F., Possani L.D. The genomic region encoding toxin gamma from the scorpion Tityus serrulatus contains an intron. FEBS Lett. 1993, 335(1):6-8.
4. Bhattacharyya N., Banerjee D. Transcriptional regulatory sequences within the first intron of the chicken apolipoproteinAI (apoAI) gene. Gene 1999, 234(2):371-380.
5. Blanc E., Hassani O., Meunier S., Mansuelle P., Sampieri F., Rochat H., Darbon H. 1H-NMR-derived secondary structure and overall fold of a natural anatoxin from the scorpion Androctonus australis hector. Eur. J. Biochem. 1997, 247(3):1118-1126.
6. Bysani N., Daugherty J.R., Cooper T.G. Saturation mutagenesis of the UASNTR (GATAA) responsible for nitrogen catabolite repression-sensitive transcriptional activation of the allantoin pathway genes in Saccharomyces cerevisiae. J. Bacteriol. 1991, 173(16):4977-4982.
7. Cao Z.-J., Luo F., Wu Y.-L., Mao X., Li W.-X. Genetic mechanisms of scorpion venom peptide diversification. Toxicon 2006, 47(3):348-355.
8. Cavener D.R. Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates. Nucleic Acids Res. 1987, 15(4):1353-1361.
9. Ceard B., Martin-Eauclaire M., Bougis P.E. Evidence for a position-specific deletion as an evolutionary link between long- and short-chain scorpion toxins. FEBS Lett. 2001, 494(3):246-248.
10. Clement J.Q., Maiti S., Wilkinson M.F. Localization and stability of introns spliced from the Pem homeobox gene. J. Biol. Chem. 2001, 276(20):16919-16930.
11. Csank C., Taylor F.M., Martindale D.W. Nuclear pre-mRNA introns: analysis and comparison of intron sequences from Tetrahymena thermophila and other eukaryotes. Nucleic Acids Res. 1990, 18(17):5133-5141.
12. Delabre M.L., Pasero P., Marilley M., Bougis P.E. Promoter structure and intron-exon organization of a scorpion alpha-toxin gene. Biochemistry 1995, 34(20):6729-6736.
13. Fedorova L., Fedorov A. Introns in gene evolution. Genetica 2003, 118(2-3):123-131.
14. Froy O., Gurevitz M. Membrane potential modulators: a thread of scarlet from plants to humans. FASEB J. 1998, 12(15):1793-1796.
15. Froy O., Sagiv T., Poreh M., Urbach D., Zilberberg N., Gurevitz M. Dynamic diversification from a putative common ancestor of scorpion toxins affecting sodium, potassium, and chloride channels. J. Mol. Evol. 1999, 48(2):187-196.
16. Goodall G.J., Filipowicz W. The AU-rich sequences present in the introns of plant nuclear pre-mRNAs are required for splicing. Cell 1989, 58(3):473-483.
17. Guan R.-J., Wang C.-G., Wang M., Wang D.-C. A depressant insect toxin with a novel analgesic effect from scorpion Buthus martensii Karsch. Biochim. Biophys. Acta. 2001, 1549(1):9-18.
18. Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987, 15(20):8125-8148.
19. Kozminsky-Atias A., Bar-Shalom A., Mishmar D., Zilberberg N. Assembling an arsenal, the scorpion way. BMC Evol. Biol. 2008, 8:333.
20. + channels. FEBS Lett. 1997, 402(1):45-49.
21. Liu Y.-F., Ma R.-L., Wang S.-L., Duan Z.-Y., Zhang J.-H., Wu L.-J., Wu C.-F. Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii Karsch in Escherichia coli. Protein Expr. Purif. 2003, 27(2):253-258.
22. Ma Y.-B., Zhao R.-M., He Y.-W., Li S.-Y., Liu J., Wu Y.-L., Cao Z.-J., Li W.-X. Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal. BMC Genomics 2009, 10:290.
23. Ma Y.-B., Zhao R.-M., Li S.-Y., Fan S.-Z., Wu Y.-L., Liu H., Cao Z.-J., Li W.-X. Characterization of LmTxLP11 and LmVP1.1 transcripts and genomic organizations: alternative splicing contributing to the diversity of scorpion venom peptides. Toxicon 2009, 53(1):129-134.
24. Mitchell P.J., Tjian R. Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science 1989, 245(4916):371-378.
25. Mount S.M., Burks C., Hertz G., Stormo G.D., White O., Fields C. Splicing signals in Drosophila: intron size, information content, and consensus sequences. Nucleic Acids Res. 1992, 20(16):4255-4262.
26. Possani L.D., Merino E., Corona M., Bolivar F., Becerril B. Peptides and genes coding for scorpion toxins that affect ion-channels. Biochimie 2000, 82(9-10):861-868.
27. + channels. Biochemistry 1997, 36(44):13473-13482.
28. + channels. Eur. J. Biochem. 1997, 245(2):457-464.
29. Ruskin B., Zamore P.D., Green M.R. A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly. Cell 1988, 52(2):207-219.
30. Schwartz E.F., Diego-Garcia E., Rodriguez de la Vega R.C., Possani L.D. Transcriptome analysis of the venom gland of the Mexican scorpion Hadrurus gertschi (Arachnida: Scorpiones). BMC Genomics 2007, 8:119.
31. Surinya K.H., Cox T.C., May B.K. Identification and characterization of a conserved erythroid-specific enhancer located in intron 8 of the human 5-aminolevulinate synthase 2 gene. J. Biol. Chem. 1998, 273(27):16798-16809.
32. Xiong Y.-M., Ling M.-H., Lan Z.-D., Wang D.-C., Chi C.-W. The cDNA sequence of an excitatory insect selective neurotoxin from the scorpion Buthus martensi Karsch. Toxicon 1999, 37(2):335-341.
33. Xiong Y.-M., Ling M.-H., Wang D.-C., Chi C.-W. The cDNA and genomic DNA sequences of a mammalian neurotoxin from the scorpion Buthus martensii Karsch. Toxicon 1997, 35(7):1025-1031.
34. Zamudio F.Z., Conde R., Arevalo C., Becerril B., Martin B.M., Valdivia H.H., Possani L.D. The mechanism of inhibition of ryanodine receptor channels by imperatoxin I, a heterodimeric protein from the scorpion Pandinus imperator. J. Biol. Chem. 1997, 272(18):11886-11894.
35. Zeng X.-C., Li W.-X., Zhu S.-Y., Peng F., Jiang D.-H., Yang F.-H., Wu K.-L. Cloning and characterization of the cDNA sequences of two venom peptides from Chinese scorpion Buthus martensii Karsch (BmK). Toxicon 2000, 38(7):893-899.
36. Zeng X.-C., Li W.-X., Zhu S.-Y., Peng F., Zhu Z.-H., Wu K.-L., Yiang F.-H. Cloning and characterization of a cDNA sequence encoding the precursor of a chlorotoxin-like peptide from the Chinese scorpion Buthus martensii Karsch. Toxicon 2000, 38(8):1009-1014.