Expression and purification of functional epitope of pigment epithelium-derived factor in E. coli with inhibiting effect on endothelial cells

Protein Journal

Volumn 29, Issue 3, 2010, Pages 167-173

  Gong, Qing ^a   Yang, Xia ^a   Cai, Weibin ^a   Gao, Guoquan ^a   Yang, Zhonghan ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Angiogenesis; Apoptosis; Epitope; HUVECs; PEDF

Indexed keywords

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; PGEX 4T 1 PROTEIN; PIGMENT EPITHELIUM DERIVED FACTOR; PIGMENT EPITHELIUM DERIVED FACTOR 34; THROMBIN; UNCLASSIFIED DRUG;

ALPHA HELIX; APOPTOSIS; ARTICLE; CELL VIABILITY; CHROMATOGRAPHY; CIRCULAR DICHROISM; ENDOTHELIUM CELL; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; MOLECULAR CLONING; NONHUMAN; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; RECOMBINANT PLASMID; SOLUBILITY; ULTRAFILTRATION; UMBILICAL VEIN; WESTERN BLOTTING;

AMINO ACID SEQUENCE; APOPTOSIS; CELL SURVIVAL; CIRCULAR DICHROISM; ENDOTHELIAL CELLS; EPITOPES; ESCHERICHIA COLI; EYE PROTEINS; FLOW CYTOMETRY; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NEOVASCULARIZATION, PATHOLOGIC; NERVE GROWTH FACTORS; RECOMBINANT FUSION PROTEINS; SERPINS; ULTRAFILTRATION; UMBILICAL VEINS;

EID: 77954141988     PISSN: 15723887     EISSN: 15734943     Source Type: Journal    
DOI: 10.1007/s10930-010-9236-6     Document Type: Article

References (26)

1. Alberdi E, Aymerich MS, Becerra SP (1999) J Biol Chem 274(44):31605-31612
2. Amaral J, Becerra SP (2009) Invest Ophthalmol Vis Sci Oct. 22 [Epub ahead of print]
3. Bernard A, Gao-Li J, Franco CA, Bouceba T, Huet A, Li Z (2009) J Biol Chem 284(16):10480-10490
4. Cai J, Parr C, Watkins G, Jiang WG, Boulton M (2006) Clin Cancer Res 12:3510-3517
5. Cao W, Tombran-Tink J, Elias R, Sezate S, Mrazek D, McGinnis JF (2001) Invest Ophthalmol Vis Sci 42:1646-1652
6. Chen YD, Zhang YB, Zhu R, Zhang FT, Jiang J, Shi Y, Zhang QY, Chen SL, Gui JF (2005) Biochem Biophys Res Commun 335(3):799-809
7. Dawson DW, Volpert OV, Gillis P, Crawford SE, Xu H, Benedict W, Bouck NP (1999) Science 285(5425):245-248
8. Filleur S, Nelius T, de Riese W, Kennedy RC (2009) J Cell Biochem 106(5):769-775
9. Filleur S, Volz K, Nelius T, Mirochnik Y, Huang H, Zaichuk TA, Aymerich MS, Becerra SP, Yap R, Veliceasa D, Shroff EH, Volpert OV (2005) Cancer Res 65(12):5144-5152
10. Halin S, Wikström P, Rudolfsson SH, Stattin P, Doll JA, Crawford SE, Bergh A (2004) Cancer Res 64:5664-5671
11. Ho TC, Chen SL, Yang YC, Liao CL, Cheng HC, Tsao YP (2007) Cardiovasc Res 76(2):213-223
12. Ho TC, Yang YC, Cheng HC, Wu AC, Chen SL, Tsao YP (2006) Biochem Biophys Res Commun 342(2):372-378
13. Matsumoto K, Ishikawa H, Nishimura D, Hamasaki K, Nakao K, Eguchi K (2004) Hepatology 40(1):252-259
14. Mirochnik Y, Aurora A, Schulze-Hoepfner FT, Deabes A, Shifrin V, Beckmann R, Polsky C, Volpert OV (2009) Clin Cancer Res 15(5):1655-1663
15. Notari L, Baladron V, Aroca-Aguilar JD, Balko N, Heredia R, Meyer C, Notario PM, Saravanamuthu S, Nueda ML, Sanchez-Sanchez F, Escribano J, Laborda J, Becerra SP (2006) J Biol Chem 281(49):38022-38037
16. Ogata N, Imaizumi M, Miyashiro M, Arichi M, Matsuoka M, Ando A, Matsumura M (2005) Am J Ophthalmol 140(5):937-939
17. Simonovic M, Gettins PG, Volz K (2001) Proc Natl Acad Sci USA 98(20):11131-11135
18. Stellmach V, Crawford SE, Zhou W, Bouck N (2001) Proc Natl Acad Sci USA 98:2593-2597
19. Tatar O, Adam A, Shinoda K, Stalmans P, Eckardt C, Lüke M, Bartz-Schmidt KU, Grisanti S (2006) Am J Ophthalmol 142(1):95-104
20. Tombran-Tink J, Chader GG, Johnson LV (1991) Exp Eye Res 53:411-414
21. Volpert OV, Zaichuk T, Zhou W, Reiher F, Ferguson TA, Stuart PM, Amin M, Bouck NP (2002) Nat Med 8(4):349-357
22. Wang JJ, Zhang SX, Lu K, Chen Y, Mott R, Sato S, Ma JX (2005) Diabetes 54(1):243-250
23. Wang QS, Yang X, Yang ZH, Gao GQ (2006) Prep Biochem Biotechnol 36(1):1-12
24. Yang H, Cheng R, Liu G, Zhong Q, Li C, Cai W, Yang Z, Ma J, Yang X, Gao G (2009) Cancer Sci 100(12):2419-2425
25. Yamagishi S, Matsui T, Nakamura K, Ueda S, Noda Y, Imaizumi T (2008) Curr Drug Targets 9(11):1025-1029
26. Zhang SX, Wang JJ, Gao G, Shao C, Mott R, Ma JX (2006) FASEB J 20:323-325