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Volumn 20, Issue 14, 2010, Pages 4128-4131

AMP-activated protein kinase (AMPK) activators from Myristica fragrans (nutmeg) and their anti-obesity effect

Author keywords

AMP activated protein kinase (AMPK); Myristica fragrans; Myristicaceae; Tetrahydrofuran lignans

Indexed keywords

FRAGRANSIN C 1; GALBACIN; MYRISTICA FRAGRANS EXTRACT; NECTANDRIN; PLANT EXTRACT; SAUCERNETINDIOL; TETRAHYDROFURAN DERIVATIVE; TETRAHYDROFUROGUAIACIN B; UNCLASSIFIED DRUG; VERRUCOSIN;

EID: 77953870889     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2010.05.067     Document Type: Article
Times cited : (94)

References (20)
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    • 77953870500 scopus 로고    scopus 로고
    • note
    • R 43.5 min).
  • 10
    • 77953873867 scopus 로고    scopus 로고
    • note
    • 13C NMR, see Supplementary Tables S1 and S2.
  • 16
    • 77953872611 scopus 로고    scopus 로고
    • note
    • 5 cells/well in 2 mL growth medium. Differentiation of C2C12 myoblasts was induced by replacing the growth medium with DMEM containing 5% horse serum when the cells reached confluence. The medium was changed every 48 h until the formation of myotubes was observed. The cells were used in the experiments at 4-5 days after differentiation.
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    • 33749336146 scopus 로고    scopus 로고
    • note
    • 79. β-Actin protein levels were used as a control for equal protein loading. The immunoreactive antigen was then recognized using a horseradish peroxidase-labeled anti-rabbit IgG and an enhanced chemiluminescence detection kit (Lee, Y. S.; Kim, W. S.; Kim, K. H.; Yoon, M. J.; Cho, H. J.; Shen, Y.; Ye, J. M.; Lee, C. H.; Oh, W. K.; Kim, C. T.; Cordula, H. B.; Gosby, A.; Kraegen, E. W.; James, D. E.; Kim, J. B. Diabetes 2006, 55, 2256).
  • 18
    • 77953871501 scopus 로고    scopus 로고
    • note
    • Animal experiments: Thirty eight-week-old male C57BL/6 mice were housed in plastic cages in a temperature-controlled (22 ± 1 °C) room and maintained on a reverse 12 h light/dark cycle. The mice were divided randomly into three groups: normal diet group (ND), high-fat diet group (HFD) with a daily gavage of the vehicle as control; and HFD + 200 mg/kg of tetrahydrofuran mixture-fed group (HFD + THF). The HFD (DIO series diets, Research Diets Inc., USA) was based on a modified Western diet and contained 21% (w/w) lard and 0.15% (w/w) cholesterol (Moon, H. S.; Lee, H. G.; Seo, J. H.; Chung, C. S.; Kim, T. G.; Choi, Y. J.; Cho, C. S. J. Nutr. Biochem. 2009, 20, 187). The tetrahydrofuran mixture was administered for 6 weeks, and the weight gain was measured every week. The food intake was measured for three consecutive days per week by subtracting the food jar pre- and post-weights for 6 weeks, and the mean values were used as the daily food intake. At the necropsy, both sides of the inguinal and epididymal adipose tissues were removed and weighed, and the relative adipose tissue weight to body weight was calculated. All animal experiments were approved by the Institutional Animal Care and Use Committee, and were performed in accordance with the institutional guidelines at AmorePacific Corporation, Korea.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.