Specific effect of 5-fluorouracil on α-fetoprotein gene expression during the in vitro mouse embryonic stem cell differentiation

International Journal of Toxicology

Volumn 29, Issue 3, 2010, Pages 297-304

  Pamies, David ^a   Vicente Salar, Néstor ^a   Sogorb, Miguel A ^a   Roche, Enrique ^a   Reig, Juan A ^a  

^a MIGUEL HERNÁNDEZ UNIVERSITY   (Spain)

Author keywords

5 fluorouracil; Embryoid bodies; Embryonic stem cells; Embryotoxicity; Extraembryonic endoderm; Primitive endoderm

Indexed keywords

ALPHA FETOPROTEIN; FLUOROURACIL; RNA;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL VIABILITY; CONCENTRATION RESPONSE; DRUG EFFECT; EMBRYO; EMBRYONIC STEM CELL; EMBRYOTOXICITY; ENDODERM; GENE EXPRESSION; GENETIC MARKER; IN VITRO STUDY; MONOLAYER CULTURE; MOUSE; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ALPHA-FETOPROTEINS; ANIMALS; ANTIGENS, DIFFERENTIATION; BETA CATENIN; CELL DIFFERENTIATION; CELL LINE; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, DRUG; EMBRYONIC STEM CELLS; ENDODERM; ENDPOINT DETERMINATION; FLUOROURACIL; GENE EXPRESSION REGULATION, DEVELOPMENTAL; MEMBRANE PROTEINS; MESODERM; MICE; MYOSIN HEAVY CHAINS; TERATOGENS; TIME FACTORS; TOXICITY TESTS; VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2;

EID: 77953834499     PISSN: 10915818     EISSN: 1092874X     Source Type: Journal    
DOI: 10.1177/1091581810366312     Document Type: Article

References (34)

1. Rolletschek A., Blyszczuk P., Wobus AM Embryonic stem cell-derived cardiac, neuronal and pancreatic cells as model systems to study toxicological effects. Toxicol Lett. 2004 ; 149 (1-3). 361-369.
2. Klug S., Lewandowski C., Nau H., Neubert D. Modification and standardization of the culture of early postimplantation embryos for toxicological studies. Arch Toxicol. 1985 ; 58 (2). 84-88.
3. Piersma AH Validation of alternative methods for developmental toxicity testing. Toxicol Lett. 2004 ; 149 (1-3). 147-153.
4. Spielmann H., Pohl I., Doering B., Liebsch M., Moldenhauer F. The embryonic stem cell test, an in vitro embryotoxicity test using two permanent mouse cell lines: 3T3 fibroblasts and embryonic stem cells. In Vitro Toxicol. 1997 ; 10 (1). 119-127.
5. Scholz G., Pohl I., Genschow E., Klemm M., Spielmann H. Embryotoxicity screening using embryonic stem cells in vitro: correlation to in vivo teratogenicity. Cells Tissues Organs. 1999 ; 165 (3-4). 203-211.
6. Genschow E., Spielmann H., Scholz G., et al. Validation of the embryonic stem cell test in the international ECVAM validation study on three in vitro embryotoxicity tests. Altern Lab Anim. 2004 ; 32 (3). 209-244. (Pubitemid 39517737)
7. Seiler AE, Buesen R., Visan A., Spielmann H. Use of murine embryonic stem cells in embryotoxicity assays: the embryonic stem cell test. Methods Mol Biol. 2006 ; 329: 371-395.
8. Festag M., Sehner C., Steinberg P., Viertel B. An in vitro embryotoxicity assay on the disturbance of the differentiation of murine embryonic stem cells into endothelial cells II. Testing of compounds. Toxicol In Vitro. 2007 ; 21 (8). 1631-1640. (Pubitemid 350086874)
9. Adler S., Pellizer C., Hareng L., Hartung T., Bremer S. First speps in establishing a developmental toxicity test method based on human embryonic stem cells. Toxicol In Vitro. 2008 ; 22 (1). 200-211.
10. Doetschman TC, Einstetter H., Katz M., Schmidt W., Kemler R. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J Embryol Exp Morphol. 1985 ; 87: 27-45.
11. Murray P., Edgar D. The regulation of embryonic stem cell differentiation by leukaemia inhibitory factor (LIF). Differentiation. 2001 ; 68 (4-5). 227-234.
12. Buehr M., Nichols J., Stenhouse F. Rapid loss of oct-4 and pluripotency in cultured rodent blastocysts and derivative cell lines. Biol Reprod. 2003 ; 68 (1). 222-229.
13. Kwon GS, Fraser ST, Eakin GS, et al. Tg(Afp-GFP) expression marks primitive endoderm lineages during mouse development. Dev Dyn. 2006 ; 235 (9). 2549-2558.
14. Hamazaki T., Oka M., Yamanaka S., Terada N. Aggregation of embryonic stem cells induces Nanog repression and primitive endoderm differentiation. J Cell Sci. 2004 ; 117 (pt 23). 5681-5686.
15. Enseñat-Waser R., Santana A., Vicente-Salar N., et al. Isolation and characterization of residual undifferentiated mouse embryonic stem cells from embryoid body cultures by fluorescence tracking. In Vitro Cell Dev Biol Anim. 2006 ; 42 (5-6). 115-123.
16. Roche E., Sepulcre P., Reig JA, Santana A., Soria B. Ectodermal commitment of insulin-producing cells derived from mouse embryonic stem cells. FASEB J. 2005 ; 19 (10). 1341-1343.
17. Freeman SJ, Brown NA Inhibition of yolk sac function in late gastrulation rat conceptuses as a cause of teratogenesis: an in vivo/in vitro study. Reprod Toxicol. 1994 ; 8 (2). 137-143.
18. Voiculescu O., Bertocchini F., Wolpert L., Keller RE, Stern CD The amniote primitive streak is defined by epithelial cell intercalation before gastrulation. Nature. 2007 ; 449 (7165). 1049-1052.
19. Chae JI, Yu K., Cho SK, et al. Aberrant expression of developmentally important signaling molecules in cloned porcine extraembryonic tissues. Proteomics. 2008 ; 8 (13). 2724-2734.
20. Paparella M., Kolossov E., Fleischmann BK, Hescheler J., Bremer S. The use of quantitative image analysis in the assessment of in vitro embryotoxicity endpoints based on a novel embryonic stem cell clone with endoderm-related GFP expression. Toxicol In Vitro. 2002 ; 16 (5). 589-597.
21. Grafton TF, Bazare JJ Jr, Hansen DK, Sheehan DM The in vitro embryotoxicity of 5-fluorouracil in rat embryos. Teratology. 1987 ; 36 (3). 371-377.
22. Kufe DW, Major PP 5-fluorouracil incorporation into human breast carcinoma RNA correlates with citotoxicity. J Biol Chem. 1981 ; 256 (19). 9802-9805.
23. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 ; 162 (1). 156-159.
24. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)). Methods. 2001 ; 25 (4). 402-408.
25. Seiler A., Visan A., Buesen R., Genschow E., Spielmann H. Improvement of an in vitro stem cell assay for developmental toxicity: the use of molecular endpoints in the embryonic stem cell test. Reprod Toxicol. 2004 ; 18 (2). 231-240.
26. Kalantry S., Manning S., Haub O., et al. The amnionless gene, essential for mouse gastrulation, encodes a visceral-endoderm- specific protein with an extracellular cysteine-rich domain. Nat Genet. 2001 ; 27 (4). 412-416.
27. Yasunaga M., Tada S., Torikai-Nishikawa S., et al. Induction and monitoring of definitive and visceral endoderm differentiation of mouse ES cells. Nat Biotechnol. 2005 ; 23 (12). 1542-1550.
28. Tada S., Era T., Furusawa C., Sakurai H., et al. Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture. Development. 2005 ; 132 (19). 4363-4374.
29. Mohamed OA, Clarke HJ, Dufort D. Beta-catenin signaling marks the prospective site of primitive streak formation in the mouse embryo. Dev Dyn. 2004 ; 231 (2). 416-424.
30. Seiler A., Buesen R., Hayess K., et al. Current status of the embryonic stem cell test: the use of recent advances in the field of stem cell technology and gene expression analysis. ALTEX. 2006 ; 23 suppl: 393-399.
31. Hartmann K-U., Heidelberger C. Studies on fluorinated pyrimidines. J Biol Chem. 1961 ; 236: 3006-3013.
32. Sun XX, Dai MS, Lu H. 5-fluorouracil activation on p53 involves an MDM2-ribosomal interaction. J Biol Chem. 2007 ; 282 (11). 8052-8059.
33. Abe K., Niwa H., Takiguchi M., et al. Endoderm-specific gene expression in embryonic stem cells differentiated to embryoid bodies. Exp Cell Res. 1996 ; 229 (1). 27-34.
34. Spielmann H., Seiler A., Bremer S., et al. The practical application of three validated in vitro embryotoxicity tests. The report and recommendations of an ECVAM/ZEBET workshop (ECVAM Workshop 57). Altern Lab Anim. 2006 ; 34 (5). 527-538.