The use of quantitative image analysis in the assessment of in vitro embryotoxicity endpoints based on a novel embryonic stem cell clone with endoderm-related GFP expression

Toxicology in Vitro

Volumn 16, Issue 5, 2002, Pages 589-597

  Paparella, M ^a   Kolossov, E ^b   Fleischmann, B K ^b   Hescheler, J ^b   Bremer, S ^a  

^a JOINT RESEARCH CENTRE   (Italy)

^b UNIVERSITY OF COLOGNE   (Germany)

Author keywords

5 Fluorouracil; Alpha fetoprotein; Animal replacement method; Caffeine; Developmental toxicity; Dexamethasone; Diphenylhydantoin; Embryonic stem cells; Endoderm; GFP; Image analysis; In vitro differentiation; Reporter gene; Saccharin

Indexed keywords

ALPHA FETOPROTEIN; CAFFEINE; DEXAMETHASONE; FLUOROURACIL; GREEN FLUORESCENT PROTEIN; PHENYTOIN; SACCHARIN;

ARTICLE; BIOASSAY; CELL CLONE; CELL MATURATION; EMBRYOTOXICITY; ENDODERM; GERM LAYER; HEART MUSCLE CELL; IMAGE ANALYSIS; IN VITRO STUDY; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REPORTER GENE; STEM CELL; TARGET ORGAN;

ALPHA-FETOPROTEINS; ANIMAL TESTING ALTERNATIVES; CELL DIFFERENTIATION; CELLS, CULTURED; CLONE CELLS; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDODERM; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; IMAGE PROCESSING, COMPUTER-ASSISTED; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; PLURIPOTENT STEM CELLS; SPHEROIDS, CELLULAR; XENOBIOTICS;

ANIMALIA;

EID: 0036800937     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(02)00052-8     Document Type: Article

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