Identification, cloning and expression of a cold-active β-galactosidase from a novel Arctic bacterium, Alkalilactibacillus ikkense

Environmental Technology

Volumn 31, Issue 10, 2010, Pages 1107-1114

  Schmidt, Mariane ^a   Stougaard, Peter ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Alkalilactibacillus; Cold active; Lactase; Psychrophilic; galactosidase

Indexed keywords

BACTERIA; BACTERIOLOGY; CLONING; ELECTROPHORESIS; ENZYMES; ESCHERICHIA COLI; GELS; PURIFICATION; SUGARS;

CLONING AND EXPRESSION; COLD-ACTIVE; CONVERSION RATES; GALACTOSIDASES; GEL ELECTROPHORESIS; GLYCOSYL HYDROLASES; GRAM-POSITIVE BACTERIUM; LOW TEMPERATURES; OPTIMAL ACTIVITY; RECOMBINANT ENZYMES; SEQUENCE SIMILARITY; STABILIZING AGENTS;

ENZYME ACTIVITY;

BETA GALACTOSIDASE; GLYCOSIDASE; LACTOSE; RECOMBINANT ENZYME;

ARCTIC ENVIRONMENT; CLONE; COLD TOLERANCE; COLIFORM BACTERIUM; ELECTROKINESIS; ENZYME; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ANALYSIS; PH; RECOMBINATION;

ALKALILACTOBACILLUS IKKENSE; ALKALINITY; ARTICLE; BACILLUS; DNA SEQUENCE; ENZYME ANALYSIS; ENZYME ISOLATION; GEL ELECTROPHORESIS; GEOBACILLUS; GRAM POSITIVE BACTERIUM; LACTOBACILLUS; LOW TEMPERATURE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PAENIBACILLUS; PROTEIN EXPRESSION;

BACILLUS (BACTERIUM); BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GEOBACILLUS; LACTOBACILLUS; PAENIBACILLUS; POSIBACTERIA;

EID: 77953800958     PISSN: 09593330     EISSN: 1479487X     Source Type: Journal    
DOI: 10.1080/09593331003677872     Document Type: Article

References (22)

1. M. Akasaki, M. Suzuki, I. Funakoshi, and I. Yamashina, Characterization of β-galactosidase from a special strain of Aspergillus oryzae, J. Biochem. 80 (1976), pp. 1195-1200.
2. K. Ohmiya, H. Ohashi, T. Kobayashi, and S. Shimizu, Hydrolysis of lactose by immobilized microorganisms, Appl. Environ. Microbiol. 33 (1977), pp. 137-146.
3. A.P. Rodríguez, R.F. Leiro, M.C. Trillo, M.E. Cerdán, M.I.G. Siso, and M. Becerra, Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase, Microb. Cell. Fact. 5 (2006), DOI:10.1186/1475-2859-5-41. (Pubitemid 46189386)
4. T. Nakagawa, Y. Fujimoto, M. Uchino, T. Miyaji, K. Takano, and N. Tomizuka, Isolation and characterization of psychrophiles producing cold-active β-galactosidase, Lett. Appl. Microbiol. 37 (2006), pp. 154-157.
5. J.A. Coker, P.P. Sheridan, J. Loveland-Curtze, K.R. Gutshall, A. Auman, and J.E. Brenchley, Biochemical characterization of a β-galactosidase with low temperature optimum obtained from an Arctic Arthrobacter isolate, J. Bacteriol. 185 (2003), pp. 5473-5482.
6. P. Karasová-Lipovová, H. Strnad, V. Spiwok, S. Malá, B. Králová, and N. Russell, The cloning, purification and characterization of a cold-active β-galactosidase from the psychrotolerant Antactic bacterium Arthrobacter sp. C2-2, Enzyme Microbiol. Technol. 33 (2003), pp. 836-844.
7. T. Nakagawa, Y. Fujimoto, R. Ikehata, T. Miyaji, and N. Tomizuka, Purification and molecular characterization of cold-active β-galactosidase from Arthrobacter psychrolactophilus strain F2, Appl. Microbiol. Biotechnol. 72 (2006), pp. 720-725.
8. J.M. Coombs and J.E. Brenchley, Biochemical and phylogenetic analyses of a cold-active beta-galactosidase from the lactic acid bacterium Carnobacterium piscicola BA, Appl. Environ. Microbiol. 65 (1999), pp. 5443-5450.
9. H. Cieśliński, J. Kur, A. Białkowska, I. Baran, K. Makowski, and M. Turkiewicz, Cloning, expression, and purification of a recombinant cold-adapted β-galactosidase from Antarctic bacterium Pseudoalteromonas sp. 22b, Protein Expr. Purif. 39 (2005), pp. 27-34.
10. S. Fernandes, B. Geueke, O. Delgado, J. Coleman, and R. Hatti-Kaul, β-galactosidase from a cold-adapted bacterium: purification, characterization and application for lactose hydrolysis, Appl. Microbiol. Biotechnol. 58 (2002), pp. 313-321.
11. A. Hoyoux, I. Jennes, P. Dubois, S. Genicot, F. Dubail, J.M. Francois, E. Baise, G. Feller, and C. Gerday, Cold-adapted β-galactosidase from the Antarctic psychrophile Pseudoalteromonas haloplanktis, Appl. Environ. Microbiol. 67 (2001), pp. 1529-1535.
12. M. Turkiewicz, J. Kur, A. Bialkowska, H. Cieslinski, H. Kalinowska, and S. Bielecki, Antarctic marine bacterium Pseudoalteromonas sp. 22b as a source of cold-adapted β-galactosidase, Biomol. Engin. 20 (2003), pp. 317-324.
13. T. Nakagawa, R. Ikehata, M. Uchino, T. Mijaji, K. Takano, and N. Tomizuka, Cold-active acid β-galactosidase activity of isolated psychrophilic basidiomycetous yeast Guehomyces pullulans, Microbiol. Res. 161 (2006), pp. 75-79.
14. M. Schmidt, A. Priemé, and P. Stougaard, High microbial diversity in permanently cold and alkaline ikaite columns from Greenland, Extremophiles 10 (2006), pp. 551-562.
15. J. Sambrook and D.W. Russell, Commonly used techniques in molecular cloning, Appendix 8. In Molecular Cloning: A Laboratory Manual, 3rd ed., Vol.3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2001.
16. P.M. Møller, F. Jørgensen, O.C. Hansen, S.M. Madsen, and P. Stougaard, Intra- and extracellular β-galactosidases from Bifidobacterium bifidum and B. infantis: Molecular cloning, heterologous expression and comparative characterization, Appl. Environ. Microbiol. 67 (2001), pp. 2276-2283.
17. J.M. Miller, Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1972.
18. H.F. Seidle, K. McKenzie, I. Marten, O. Shoseyov, and R.E. Huber, Trp-262 is a key residue for the hydrolytic and transglucosidic reactivity of the Aspergillus niger family 3 β-galactosidase: Substitution results in enzymes with mainly transglucosidic activity, Arch. Biochem. Biophys. 444 (2005), pp. 66-75.
19. X.-L. Chen, Y.-Z. Zhang, P.-J. Gao, and X.-W. Luan, Two different proteases produced by a deep-sea psychrotrophic bacterial strain, Pseudoalteromonas sp. SM9913, Mar. Biol. 143 (2003), pp. 989-993.
20. J.C. Gebler, R. Aebersold, and S.G. Withers, Glu-537, not Glu-461, is the nucleophile in the active site of (LacZ) β-galactosidase from Escherichia coli, J. Biol. Chem. 267 (1992), pp. 11126-11130.
21. G. Feller and C. Gerday, Psychrophilic enzymes: molecular basis of cold adaptation, Cell. Mol. Life. Sci. 53 (1997), pp. 830-841. (Pubitemid 27507534)
22. J. Coombs and J.E. Brenchley, Characterization of two new glycosyl hydrolases from the lactic acid bacterium Carnobacterium piscicola Strain BA, Appl. Environ. Microbiol. 67 (2001), pp. 5094-5099.