Purification and characterization of a novel extracellular protease from a halo-alkaliphilic Bacillus sp. 17N-1, active in polar organic solvents

Environmental Technology

Volumn 31, Issue 10, 2010, Pages 1073-1082

  Papamichael, E M ^a   Theodorou, L G ^a   Perisynakis, A ^a   Drainas, C ^a  

^a UNIVERSITY OF IOANNINA   (Greece)

Author keywords

Cysteine proteases; Enzyme kinetics; Halo alkaliphiles; Polar organic solvents; Purification

Indexed keywords

ACETONITRILE; ACTIVATION ENERGY; AMINO ACIDS; BACTERIOLOGY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYMES; PHOSPHATASES; PURIFICATION; RATE CONSTANTS; SODIUM CHLORIDE;

BACILLUS SP; CYSTEINE PROTEASE; CYSTEINE PROTEASES; DITHIOTHREITOL; EXTRACELLULAR PROTEASE; HALO-ALKALIPHILES; ION PAIRS; METALLIC IONS; MICHAELIS-MENTEN; MICHAELIS-MENTEN KINETIC; POLAR ORGANIC SOLVENTS; SERINE PROTEASE;

ORGANIC SOLVENTS;

3,4 DICHLOROISOCOUMARIN; ACETONITRILE; CYSTEINE PROTEINASE; DIMETHYL SULFOXIDE; DITHIOTHREITOL; EDETIC ACID; METAL ION; ORGANIC SOLVENT; PROTEINASE; SERINE PROTEINASE;

BACTERIUM; BIOTECHNOLOGY; CATALYSIS; CATION; EDTA; ENZYME ACTIVITY; HYDROLYSIS; INHIBITOR; MASS; PH; REACTION KINETICS; SODIUM CHLORIDE; SOLVENT;

ARTICLE; BACILLUS; ENZYME ANALYSIS; ENZYME PURIFICATION; MICHAELIS MENTEN KINETICS; MOLECULAR WEIGHT; NONHUMAN; PH; PKA; TEMPERATURE;

BACILLUS SP.;

EID: 77953736256     PISSN: 09593330     EISSN: 1479487X     Source Type: Journal    
DOI: 10.1080/09593331003664136     Document Type: Article

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