Influence of phosphorylation of THR-3, SER-11, and SER-15 on deoxycytidine kinase activity and stability

Nucleosides, Nucleotides and Nucleic Acids

Volumn 29, Issue 4-6, 2010, Pages 404-407

  Smal, C ^a   Ntamashimikiro, S ^a   Arts, A ^a   Van Den Neste, E ^a,b   Bontemps, F ^a  

^a DE DUVE INSTITUTE   (Belgium)

^b CLINIQUES UNIVERSITAIRES SAINT LUC   (Belgium)

Author keywords

Deoxycytidine kinase; HEK 293T cells; phosphorylation sites; site directed mutagenesis

Indexed keywords

AMINO ACID; DEOXYCYTIDINE KINASE; SERINE; SERINE 11; SERINE 15 PROTEIN; SERINE 74; THREONINE; THREONINE 3; UNCLASSIFIED DRUG;

ARTICLE; EUKARYOTIC CELL; HUMAN; HUMAN CELL; IN VIVO STUDY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PHOSPHORYLATION; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS;

CELL LINE; DEOXYCYTIDINE KINASE; ENZYME STABILITY; HUMANS; MUTAGENESIS, SITE-DIRECTED; PHOSPHORYLATION; SERINE; STRUCTURE-ACTIVITY RELATIONSHIP; THREONINE;

EUKARYOTA;

EID: 77953695306     PISSN: 15257770     EISSN: 15322335     Source Type: Journal    
DOI: 10.1080/15257771003741216     Document Type: Article

References (11)

1. Arner, E.S.; Eriksson, S. Mammalian deoxyribonucleoside kinases. Pharmacol. Ther. 1995, 67, 155-186.
2. Spasokoukotskaja, T.; Sasvari-Szekely, M.; Keszler, G.; Albertioni, F.; Eriksson, S.; Staub,M. Treatment of normal and malignant cells with nucleoside analogues and etoposide enhances deoxycytidine kinase activity. Eur. J. Cancer 1999, 35, 1862-1867.
3. Sasvari-Szekely, M.; Spasokoukotskaja, T.; Szoke, M.; Csapo, Z.; Turi, A.; Szanto, I.; Eriksson, S.; Staub, M. Activation of deoxycytidine kinase during inhibition of DNA synthesis by 2-chloro-2 - deoxyadenosine (Cladribine) in human lymphocytes. Biochem. Pharmacol. 1998, 56, 1175-1179.
4. Van Den Neste, E.; Smal, C.; Cardoen, S.; Delacauw, A.; Frankard, J.; Ferrant, A.; Van den Berghe, G.; Bontemps, F. Activation of deoxycytidine kinase by UV-C-irradiation in chronic lymphocytic leukemia B-lymphocytes. Biochem. Pharmacol. 2003, 65, 573-580.
5. Smal, C.; Vertommen, D.; Bertrand, L.; Ntamashimikiro, S.; Rider, M.H.; Van Den Neste, E.; Bontemps, F. Identification of in vivo phosphorylation sites on human deoxycytidine kinase. Role of Ser-74 in the control of enzyme activity. J. Biol. Chem. 2006, 281, 4887-4893.
6. Smal, C.; Van Den Neste, E.; Maerevoet, M.; Poire, X.; Theate, I.; Bontemps, F. Positive regulation of deoxycytidine kinase activity by phosphorylation of Ser-74 in B-cell chronic lymphocytic leukaemia lymphocytes. Cancer Lett. 2007, 253, 68-73.
7. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
8. Johnson, L.N.; Barford, D. Electrostatic effects in the control of glycogen phosphorylase by phosphorylation. Protein Sci. 1994, 3, 1726-1730.
9. Yousefi, R.; Shchutskaya, Y.Y.; Zimny, J.; Gaudin, J.C.; Moosavi-Movahedi, A.A.; Muronetz, V.I.; Zuev, Y.F.; Chobert, J.M.; Haertle, T. Chaperone-like activities of different molecular forms of beta-casein. Importance of polarity of N-terminal hydrophilic domain. Biopolymers 2009, 91, 623-632.
10. Aviram, S.; Simon, E.; Gildor, T.; Glaser, F.; Kornitzer, D. Autophosphorylation-induced degradation of the Pho85 cyclin Pcl5 is essential for response to amino acid limitation. Mol. Cell. Biol. 2008, 28, 6858-6869.
11. Johnson, L.N.; Barford, D. The effects of phosphorylation on the structure and function of proteins. Annu. Rev. Biophys. Biomol. Struct. 1993, 22, 199-232.