Duplex real-time reverse transcriptase PCR to determine cytokine mRNA expression in a hamster model of New World cutaneous leishmaniasis

BMC Immunology

Volumn 11, Issue , 2010, Pages

  Espitia, Claudia M ^a,b   Zhao, Weiguo ^a,b   Saldarriaga, Omar ^a,b   Osorio, Yaneth ^a,b   Harrison, Lisa M ^c   Cappello, Michael ^c   Travi, Bruno L ^a,b   Melby, Peter C ^a,b,d  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b Mays Cancer Center at UT Health San Antonio   (United States)

^c YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOKINE RECEPTOR CCR4; CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 10; INTERLEUKIN 12P40; INTERLEUKIN 13; INTERLEUKIN 21; INTERLEUKIN 4; MACROPHAGE DERIVED CHEMOKINE; MESSENGER RNA; THYMUS AND ACTIVATION REGULATED CHEMOKINE; TRANSCRIPTION FACTOR FOXP3; TRANSFORMING GROWTH FACTOR BETA; TUMOR NECROSIS FACTOR ALPHA; COMPLEMENTARY DNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CYTOKINE PRODUCTION; DUPLEX REAL TIME REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; EXPERIMENTAL MODEL; FEMALE; GENE EXPRESSION; IMMUNE RESPONSE; MOLECULE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKIN LEISHMANIASIS; SYNTHESIS; SYRIAN HAMSTER; ANIMAL; DISEASE COURSE; DISEASE MODEL; GENE EXPRESSION REGULATION; GENETICS; HAMSTER; LEISHMANIA; LYMPH NODE; METABOLISM; METHODOLOGY; PARASITOLOGY; PATHOLOGY; PHYSIOLOGY; REPRODUCIBILITY; SKIN; STANDARD;

ANIMALS; CRICETINAE; CYTOKINES; DISEASE MODELS, ANIMAL; DISEASE PROGRESSION; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION; LEISHMANIA GUYANENSIS; LEISHMANIASIS, CUTANEOUS; LYMPH NODES; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SKIN;

EID: 77953668084     PISSN: None     EISSN: 14712172     Source Type: Journal    
DOI: 10.1186/1471-2172-11-31     Document Type: Article

References (47)

1. Hommel M, Jaffe CL, Travi B, Milon G. Experimental models for leishmaniasis and for testing anti-leishmanial vaccines. Ann Trop Med Parasitol 1995, 89(Suppl 1):55-73.
2. Requena JM, Soto M, Doria MD, Alonso C. Immune and clinical parameters associated with Leishmania infantum infection in the golden hamster model. Vet Immunol Immunopathol 2000, 76:269-81. 10.1016/S0165-2427(00)00221-X, 11044559.
3. Travi BL, Osorio Y, Saravia NG. The inflammatory response promotes cutaneous metastasis in hamsters infected with Leishmania (Viannia) panamensis. J Parasitol 1996, 82:454-7. 10.2307/3284085, 8636852.
4. Martinez JE, Travi BL, Valencia AZ, Saravia NG. Metastatic capability of Leishmania (Viannia) panamensis and Leishmania (Viannia) guyanensis in golden hamsters. J Parasitol 1991, 77:762-8. 10.2307/3282713, 1919926.
5. Melby PC, Chandrasekar B, Zhao W, Coe JE. The hamster as a model of human visceral leishmaniasis: progressive disease and impaired generation of nitric oxide in the face of a prominent Th1-like response. J Immunol 2001, 166:1912-20.
6. Melby PC, Tryon VV, Chandrasekar B, Freeman GL. Cloning of Syrian hamster (Mesocricetus auratus) cytokine cDNAs and analysis of cytokine mRNA expression in experimental visceral leishmaniasis. Infect Immun 1998, 66:2135-42. 108174, 9573100.
7. Bilate AM, Salemi VM, Ramires FJ, de Brito T, Silva AM, Umezawa ES, Mady C, Kalil J, Cunha-Neto E. The Syrian hamster as a model for the dilated cardiomyopathy of Chagas' disease: a quantitative echocardiographical and histopathological analysis. Microbes Infect 2003, 5:1116-24. 10.1016/j.micinf.2003.07.001, 14554253.
8. Rigothier MC, Khun H, Tavares P, Cardona A, Huerre M, Guillen N. Fate of Entamoeba histolytica during establishment of amoebic liver abscess analyzed by quantitative radioimaging and histology. Infect Immun 2002, 70:3208-15. 10.1128/IAI.70.6.3208-3215.2002, 128000, 12011016.
9. Kajdacsy-Balla A, Howeedy A, Bagasra O. Syphilis in the Syrian hamster. A model of human venereal and congenital syphilis. Am J Pathol 1987, 126:599-601. 1899634, 3826303.
10. Haake DA. Hamster model of leptospirosis. Curr Protoc Microbiol 2006, Chapter 12(Unit 12E 2). 2667198, 18770576.
11. Campen MJ, Milazzo ML, Fulhorst CF, CJ Obot A, Koster F. Characterization of shock in a hamster model of hantavirus infection. Virology 2006, 356:45-9. 10.1016/j.virol.2006.07.044, 16950493.
12. Paessler S, Aguilar P, Anishchenko M, Wang HQ, Aronson J, Campbell G, Cararra AS, Weaver SC. The hamster as an animal model for eastern equine encephalitis--and its use in studies of virus entrance into the brain. J Infect Dis 2004, 189:2072-6. 10.1086/383246, 15143475.
13. Tesh RB, Guzman H, AP da R, Vasconcelos PF, Dias LB, Bunnell JE, Zhang H, Xiao SY. Experimental yellow fever virus infection in the Golden Hamster (Mesocricetus auratus). I. Virologic, biochemical, and immunologic studies. J Infect Dis 2001, 183:1431-6. 10.1086/320199, 11319679.
14. Xiao SY, Zhang H, Guzman H, Tesh RB. Experimental yellow fever virus infection in the Golden hamster (Mesocricetus auratus). II. Pathology. J Infect Dis 2001, 183:1437-44. 10.1086/320200, 11319680.
15. Xiao SY, Guzman H, Zhang H, AP Travassos da R, Tesh RB. West Nile virus infection in the golden hamster (Mesocricetus auratus): a model for West Nile encephalitis. Emerg Infect Dis 2001, 7:714-21. 10.3201/eid0704.010420, 2631753, 11585537.
16. Wong KT, Grosjean I, Brisson C, Blanquier B, Fevre-Montange M, Bernard A, Loth P, Georges-Courbot MC, Chevallier M, Akaoka H, et al. A golden hamster model for human acute Nipah virus infection. Am J Pathol 2003, 163:2127-37. 1892425, 14578210.
17. Held MR, Bungiro RD, Harrison LM, Hamza I, Cappello M. Dietary iron content mediates hookworm pathogenesis in vivo. Infect Immun 2006, 74:289-95. 10.1128/IAI.74.1.289-295.2006, 1346670, 16368983.
18. Mendez S, Valenzuela JG, Wu W, Hotez PJ. Host cytokine production, lymphoproliferation, and antibody responses during the course of Ancylostoma ceylanicum infection in the Golden Syrian hamster. Infect Immun 2005, 73:3402-7. 10.1128/IAI.73.6.3402-3407.2005, 1111885, 15908367.
19. Travi BL, Osorio Y, Melby PC, Chandrasekar B, Arteaga L, Saravia NG. Gender is a major determinant of the clinical evolution and immune response in hamsters infected with Leishmania spp. Infect Immun 2002, 70:2288-96. 10.1128/IAI.70.5.2288-2296.2002, 127932, 11953362.
20. Jittimanee J, Sermswan RW, Puapairoj A, Maleewong W, Wongratanacheewin S. Cytokine expression in hamsters experimentally infected with Opisthorchis viverrini. Parasite Immunol 2007, 29:159-67. 10.1111/j.1365-3024.2006.00929.x, 17266743.
21. Rama Iniguez S, Dea-Ayuela MA, Sanchez-Brunete JA, Torrado JJ, Alunda JM, Bolas-Fernandez F. Real-time reverse transcription-PCR quantification of cytokine mRNA expression in golden Syrian hamster infected with Leishmania infantum and treated with a new amphotericin B formulation. Antimicrob Agents Chemother 2006, 50:1195-201. 10.1128/AAC.50.4.1195-1201.2006, 1426985, 16569829.
22. Vernel-Pauillac F, Merien F. Proinflammatory and immunomodulatory cytokine mRNA time course profiles in hamsters infected with a virulent variant of Leptospira interrogans. Infect Immun 2006, 74:4172-9. 10.1128/IAI.00447-06, 1489750, 16790792.
23. Sher A, Coffman RL. Regulation of immunity to parasites by T cells and T cell-derived cytokines. Annu Rev Immunol 1992, 10:385-409. 10.1146/annurev.iy.10.040192.002125, 1590992.
24. Shannon MF, Coles LS, Attema J, Diamond P. The role of architectural transcription factors in cytokine gene transcription. J Leukoc Biol 2001, 69:21-32.
25. Sparkman L, Boggaram V. Nitric oxide increases IL-8 gene transcription and mRNA stability to enhance IL-8 gene expression in lung epithelial cells. Am J Physiol Lung Cell Mol Physiol 2004, 287:L764-73. 10.1152/ajplung.00165.2004, 15169673.
26. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990, 215:403-10.
27. Ginzinger DG. Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp Hematol 2002, 30:503-12. 10.1016/S0301-472X(02)00806-8, 12063017.
28. Liu W, Saint DA. A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics. Anal Biochem 2002, 302:52-9. 10.1006/abio.2001.5530, 11846375.
29. Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000, 25:169-93. 10.1677/jme.0.0250169, 11013345.
30. Medhurst AD, Harrison DC, Read SJ, Campbell CA, Robbins MJ, Pangalos MN. The use of TaqMan RT-PCR assays for semiquantitative analysis of gene expression in CNS tissues and disease models. J Neurosci Methods 2000, 98:9-20. 10.1016/S0165-0270(00)00178-3, 10837866.
31. Wong ML, Medrano JF. Real-time PCR for mRNA quantitation. Biotechniques 2005, 39:75-85. 10.2144/05391RV01, 16060372.
32. Pfaffl MW. A new mathematical model for relative quantification in realtime RT-PCR. Nucleic. Acids Res 2001, 29:e45.
33. Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 2002, 30:e36. 10.1093/nar/30.9.e36, 113859, 11972351.
34. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001, 25:402-8. 10.1006/meth.2001.1262, 11846609.
35. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc 2008, 3:1101-8. 10.1038/nprot.2008.73, 18546601.
36. Laskay T, Diefenbach A, Rollinghoff M, Solbach W. Early parasite containment is decisive for resistance to Leishmania major infection [published erratum appears in Eur J Immunol 1995 Sep;25(9):2726]. Eur J Immunol 1995, 25:2220-7. 10.1002/eji.1830250816, 7664785.
37. Scharton-Kersten T, Scott P. The role of the innate immune response in Th1 cell development following Leishmania major infection. J Leukoc Biol 1995, 57:515-22.
38. Sacks D, Noben-Trauth N. The immunology of susceptibility and resistance to Leishmania major in mice. Nat Rev Immunol 2002, 2:845-58. 10.1038/nri933, 12415308.
39. Alferink J, Lieberam I, Reindl W, Behrens A, Weiss S, Huser N, Gerauer K, Ross R, Reske-Kunz AB, Ahmad-Nejad P, et al. Compartmentalized production of CCL17 in vivo: strong inducibility in peripheral dendritic cells contrasts selective absence from the spleen. J Exp Med 2003, 197:585-99. 10.1084/jem.20021859, 2193819, 12615900.
40. Rosas LE, Keiser T, Barbi J, Satoskar AA, Septer A, Kaczmarek J, Lezama-Davila CM, Satoskar AR. Genetic background influences immune responses and disease outcome of cutaneous L. mexicana infection in mice. Int Immunol 2005, 17:1347-57. 10.1093/intimm/dxh313, 16141242.
41. Lieberam I, Forster I. The murine beta-chemokine TARC is expressed by subsets of dendritic cells and attracts primed CD4+ T cells. Eur J Immunol 1999, 29:2684-94. 10.1002/(SICI)1521-4141(199909)29:09<2684::AID-IMMU2684>3.0.CO;2-Y, 10508243.
42. Ng LG, Hsu A, Mandell MA, Roediger B, Hoeller C, Mrass P, Iparraguirre A, Cavanagh LL, Triccas JA, Beverley SM, et al. Migratory dermal dendritic cells act as rapid sensors of protozoan parasites. PLoS Pathog 2008, 4:e1000222. 10.1371/journal.ppat.1000222, 2583051, 19043558.
43. Cavassani KA, Campanelli AP, Moreira AP, Vancim JO, Vitali LH, Mamede RC, Martinez R, Silva JS. Systemic and local characterization of regulatory T cells in a chronic fungal infection in humans. J Immunol 2006, 177:5811-8.
44. Imai T, Nagira M, Takagi S, Kakizaki M, Nishimura M, Wang J, Gray PW, Matsushima K, Yoshie O. Selective recruitment of CCR4-bearing Th2 cells toward antigen-presenting cells by the CC chemokines thymus and activation-regulated chemokine and macrophage-derived chemokine. Int Immunol 1999, 11:81-8. 10.1093/intimm/11.1.81, 10050676.
45. Lopes JE, Soper DM, Ziegler SF. Foxp3 is required throughout the life of a regulatory T cell. Sci STKE 2007, 2007:pe36. 10.1126/stke.3932007pe36, 17609478.
46. Roy G, Dumas C, Sereno D, Wu Y, Singh AK, Tremblay MJ, Ouellette M, Olivier M, Papadopoulou B. Episomal and stable expression of the luciferase reporter gene for quantifying Leishmania spp. infections in macrophages and in animal models. Mol Biochem Parasitol 2000, 110:195-206. 10.1016/S0166-6851(00)00270-X, 11071276.
47. Dondji B, Bungiro RD, Harrison LM, Vermeire JJ, Bifulco C, D McMahon- P, Cappello M. Role for nitric oxide in hookworm-associated immune suppression. Infect Immun 2008, 76:2560-7. 10.1128/IAI.00094-08, 2423093, 18347036.