Generation of human PTH1R construct with flag epitope located internally: Comparison of two-fragment assembly by using PCR overlap extension or ligase

Journal of Biomolecular Techniques

Volumn 20, Issue 4, 2009, Pages 195-200

  Wang, Bin ^a   Yang, Yanmei ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Ligation; Polymerase chain reaction; Protein expression; Type I PTH PTH related peptide receptor

Indexed keywords

EPITOPE; LIGASE; PARATHYROID HORMONE RELATED PROTEIN RECEPTOR; PARATHYROID HORMONE[1-34];

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; EXON; HOMEOSTASIS; HUMAN; HUMAN CELL; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION;

ADENYLATE CYCLASE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACTERIOPHAGE T4; BASE SEQUENCE; CELL LINE; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA LIGASES; EXONS; GENES, SYNTHETIC; HUMANS; KIDNEY; MOLECULAR SEQUENCE DATA; PEPTIDES; POLYMERASE CHAIN REACTION; PROTEIN BIOSYNTHESIS; RECEPTOR, PARATHYROID HORMONE, TYPE 1; RECOMBINANT FUSION PROTEINS; SEQUENCE DELETION; TRANSFECTION;

EID: 77953660372     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Friedman PA. PTH revisited. Kidney Int Suppl 2004;October:S13-S19.
2. Wang B, Yang Y, Friedman PA. Na/H exchange regulatory factor 1, a novel AKT-associating protein, regulates extracellular signal-regulated kinase signaling through a B-Raf-mediated pathway. Mol Biol Cell 2008;19:1637-1645.
3. Wang B, Bisello A, Yang Y, Romero GG, Friedman PA. NHERF1 regulates parathyroid hormone receptor membrane retention without affecting recycling. J Biol Chem 2007;282:36214-36222.
4. Daunt DA, Hurt C, Hein L, Kallio J, Feng F, Kobilka BK. Subtype-specific intracellular trafficking of α2-adrenergic receptors. Mol Pharmacol 1997;51:711-720.
5. Seck T, Baron R, Horne WC. Binding of filamin to the C-terminal tail of the calcitonin receptor controls recycling. J Biol Chem 2003;278:10408-10416.
6. Brock C, Oueslati N, Soler S, Boudier L, Rondard P, Pin JP. Activation of a dimeric metabotropic glutamate receptor by inter-subunit rearrangement. J Biol Chem 2007;282:33000-33008.
7. Lee C, Gardella TJ, Abou-Samra AB, et al. Role of the extracellular regions of the parathyroid hormone (PTH)/PTH-related peptide receptor in hormone binding. Endocrinology 1994;135:1488-1495.
8. Tazawa H, Takahashi S, Zilliacus J. Interaction of the parathyroid hormone receptor with the 14-3-3 protein. Biochim Biophys Acta 2003;1620:32-38.
9. Wang B, Ma L, Tao X, Lipsky PE. Triptolide, an active component of the Chinese herbal remedy Tripterygium wilfordii Hook F, inhibits production of nitric oxide by decreasing inducible nitric oxide synthase gene transcription. Arthritis Rheum 2004;50:2995-2303.
10. Schneider H, Harbottle RP, Yokosaki Y, Kunde J, Sheppard D, Coutelle C. A novel peptide, PLAEIDGIELTY, for the targeting of α9β1-integrins. FEBS Lett 1998;429:269-273.
11. Sorensen HP, Mortensen KK. Advanced genetic strategies for recombinant protein expression in Escherichia coli. J Biotechnol 2005;115:113-128.
12. Liang Q, Chen L, Fulco AJ. An efficient and optimized PCR method with high fidelity for site-directed mutagenesis. PCR Methods Appl 1995;4:269-274.
13. Jensen PH, Weilguny D. Combination primer polymerase chain reaction for multi-site mutagenesis of close proximity sites. J Biomol Tech 2005;16:336-340.
14. Sneddon WB, Liu F, Gesek FA, Friedman PA. Obligate mitogen-activated protein kinase activation in parathyroid hormone stimulation of calcium transport but not calcium signaling. Endocrinology 2000;141:4185-4193.