The ABRF edman sequencing research group 2008 study: Investigation into homopolymeric amino acid N-terminal sequence tags and their effects on automated edman degradation

Journal of Biomolecular Techniques

Volumn 20, Issue 4, 2009, Pages 216-225

  Thoma, R S ^a   Smith, J S ^b   Sandoval, W ^c   Leone, J W ^d   Hunziker, P ^e   Hampton, B ^f   Linse, K D ^g   Denslow, N D ^h  

^a MONSANTO COMPANY   (United States)

^b UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

^c GENENTECH INC   (United States)

^d PFIZER INC   (United States)

^e UNIVERSITY OF ZURICH   (Switzerland)

^f UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^g UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^h UNIVERSITY OF FLORIDA   (United States)

Author keywords

Chemistry; Histidine; Human growth hormone; Initial yield; Repetitive yield

Indexed keywords

ALANINE; GROWTH HORMONE; POLYALANINE TAG; POLYHISTIDINE TAG; POLYMER; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CHELATION; CLINICAL RESEARCH; CONTROLLED STUDY; HOSPITAL LABORATORY; PROTEIN DEGRADATION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

AFFINITY LABELS; AMINO ACID SEQUENCE; AMINO ACIDS; AUTOMATION; EFFICIENCY; HISTIDINE; HUMAN GROWTH HORMONE; HUMANS; LABORATORIES; MOLECULAR SEQUENCE DATA; ORGANOPHOSPHORUS COMPOUNDS; PEPTIDES; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, PROTEIN; TRANSFECTION;

EID: 77953653402     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Hunkapillar M, Hewick RM, Dreyer WJ, Hood LE. High-sensitivity sequencing with a gas phased sequencer. Met. Enzymol 1983;91:399-413.
2. Porath J. Immobilized metal ion affinity chromatography. Protein Expr Purif 1992;3:263-281.
3. PROCISE®, PROCISE® cLC, and PROCISE® C Protein Sequencing Systems User Guide, Rev. A., Applied Biosystems, Part #4340645, 2003.
4. Brune DC, Hampton B, Kobayashi R, et al. ABRF ESRG 2006 Study: Edman sequencing as a method for polypeptide quantitation. J Biomol Tech 2007;18:306-320.
5. Blombäck B, Blombäck M, Hessel B, Iwanaga S. Structure of N-terminal fragments of fibrinogen and specificity of thrombin. Nature 1967;215:1445-1448.
6. Thomsen J, Kristiansen K, Brunfeldt K. The amino acid sequence of human glucagon. FEBS Lett 1972;21:315-319.
7. Walker JE, Carne AF, Runswick MJ, Harris JL, Bridgen JD. Glyceraldehyde-3-phosphate dehydrogenase: complete aminoacid sequence of the enzyme from Bacillus stearothermophilus. Eur J Biochem 1980;108:549-565.
8. Kingston IB, Kingston BL, Putnam FW. Primary structure of a histidine-rich proteolytic fragment of human ceruloplasmin. I. Amino acid sequence of the cyanogens bromide peptides. J Biol Chem 1980;255:2878-2885.
9. Hunkapiller MW, Granlund-Moyer K, Whiteley NW. Gas-phase protein/peptide sequencer. In Shively JE (ed): Methods of Protein Microcharacterization, 1986;8:223-247.