ABRF ESRG 2006 study: Edman sequencing as a method for polypeptide quantitation

Journal of Biomolecular Techniques

Volumn 18, Issue 5, 2007, Pages 306-320

  Brune, D C ^a   Hampton, B ^b   Kobayashi, R ^c   Leone, J W ^d   Linse, K D ^e   Pohl, J ^f   Thoma, R S ^g   Denslow, N D ^h  

^a ARIZONA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

^d PFIZER INC   (United States)

^e UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^f EMORY UNIVERSITY   (United States)

^g MONSANTO COMPANY   (United States)

^h UNIVERSITY OF FLORIDA   (United States)

Author keywords

Edman sequencing; Phenylthiohydantoin (PTH) amino acid; Polypeptide quantitation; Quantitative analysis

Indexed keywords

PEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; COMPARATIVE STUDY; INSTRUMENTATION; MASS SPECTROMETRY; MOLECULAR GENETICS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STANDARD; VALIDATION STUDY;

AMINO ACID SEQUENCE; MOLECULAR SEQUENCE DATA; PEPTIDES; SEQUENCE ANALYSIS, PROTEIN; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 38949159805     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. Niece RL, Williams KR, Wadsworth CL, Elliott J, Stone KL, McMurray WJ, et al. A. Synthetic peptide for evaluating protein sequencer and amino acid analyzer performance in core facilities: Design and results. In TE Hugli, ed, Techniques in Protein Chemistry, Academic Press, San Diego, pp. 89-101 (1989).
2. Speicher DW, Grant GA, Niece RL, Blacher RW, Fowler AV, Williams KR. Design, characterization and results of ABRF-89SEQ: A test sample for evaluating protein sequencer performance in protein microchemistry core facilities. In TE Hugli, ed, Current Research in Protein Chemistry, Academic Press, San Diego, pp. 159-166 (1990).
3. Yuksel KU, Grant GA, Mende-Muller LM, Niece RL, Williams KR, Speicher DW. Protein sequencing from polyvinylidene difluoride membranes: Design and characterization of a test sample (ABRF-90SEQ) and evaluation of results. In JJ Villafranca, ed, Techniques in Protein Chemistry II, Academic Press, San Diego, pp. 151-162 (1991).
4. Crimmins DL, Grant GA, Mende-Muller LM, Niece RL, Slaughter C, Speicher DW, et al. Evaluation of protein sequencing core facilities: Design, characterization, and results from a test sample (ABRF-91SEQ). In RH Angeletti, ed, Techniques in Protein Chemistry III, Academic Press, San Diego, pp. 35-35 (1992).
5. Mische SM, Yuksel KU, Mende-Muller LM, Matsudaira P, Crimmins DL, Andrews PC. Protein sequencing of posttranslationally modified peptides and proteins: Design, characterization and results of ABRF-92SEQ. In RH Angeletti, ed, Techniques in Protein Chemistry IV, Academic Press, San Diego, pp. 453-461 (1993).
6. Rush J, Andrews PC, Crimmins DL, Gambee JE, Grant GA, Mische SM, et al. A synthetic peptide for evaluating protein sequencing capabilities: Design of ABRF-93SEQ and results. In JW Crabb, ed, Techniques in Protein Chemistry V, Academic Press, San Diego, pp. 133-141 (1994).
7. Gambee JE, Andrews PC, Grant GA, Merrill B, Mische SM, Rush J. Assignment of cysteine and tryptophan residues during protein sequencing: Results of ABRF-94SEQ. In JW Crabb, ed, Techniques in Protein Chemistry VI, Academic Press, San Diego, pp. 209-217 (1995).
8. DeJongh KS, Fernandez J, Gambee JE, Grant GA, Merrill B, Stone KL, et al. Design and analysis of ABRF-95SEQ, a recombinant protein with sequence heterogeneity. In D Marshak, ed, Techniques in Protein Chemistry VII, Academic Press, San Diego, pp. 347-358 (1996).
9. Fernandez J, Admon A, DeJongh K, Grant G, Henzel W, Lane WS, et al. Evaluation of ABRF-96SEQ: A sequence assignment exercise. In DR Marshak, ed, Techniques in Protein Chemistry VIII, Academic Press, San Diego, pp. 69-78 (1997).
10. Stone K, Fernandez J, Admon A, Henzel W, Lane W, Rohde M, et al. ABRF-97SEQ: Sequencing results of a low-level sample. J Biomol Tech 1999;10:26-32.
11. Carr S, Crabb J, Davis G, De Jongh K, Dupont D, Lee T, et al. ABRF-99SEQ: Analysis of a peptide and protein. Presented at ABRF'99: Bioinformatics and Biomolecular Technologies: Linking Genomes, Proteomes, and Biochemistry, Durham, NC (March 1999).
12. Henzel W, Admon A, Carr S, Davis G, De Jongh K, Lane W, et al. ABRF-98SEQ: Evaluation of peptide sequencing at high sensitivity. J Biomol Tech 2000;11:92-99.
13. Carr J, Crabb J, Davis G, Dunbar B, Dupont D, Lee T, et al. ABRF-00SEQ: Sequence analysis of a post-translationally modified peptide. Presented at ABRF'00: From Singular to Global Analysis of Biological Systems, Bellevue, Washington, (February 2000).
14. Buckel SD, Cook RG, Crawford JM, Denslow N, Fernandez J, Madden BJ, et al. ABRF-2002ESRG, a difficult sequence: Analysis of a PVDF-bound known protein with a heterogenous aminoterminus. J Biomol Tech 2002;13:246-257.
15. Buckel SB, Cook RG, Crawford JM, Denslow N, Fernandez J, Madden BJ, et al. ABRF-ESRG'03: Analysis of a PVDF-bound known protein with a homogeneous amino-terminus. J Biomol Tech 2003;14:278-288.
16. Brune D, Crawford JM, Cook RG, Denslow ND, Kobayashi R, Madden BJ, et al. ABRF ESRG 2004 Study: Modified amino acids in Edman sequencing. J Biomol Tech 2005;16:272-284.
17. Brune D, Denslow ND, Kobayashi R, Lane WS, Leone JW, Madden BJ, et al. ABRF ESRG 2005 Study: Identification of seven modified amino acids by Edman Sequencing. J Biomol Tech 2006;17:308-326.
18. Smithies O, Gibson D, Fanning EM, Goodfliesh RM, Gilman JG, Ballantyne DL. Quantitative procedures for use with the EdmanBegg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac. Biochem 1971;10:4912-4921.
19. Brown K. Peroxides in sequencing solvents: Effects on PTH-amino acids and how to detect them. ABRF News 1995;6(2):13-15.
20. Nelson RW, McLean MA, Hutchens TW. Quantitative determination of proteins by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. Anal Chem 1994;66:1408-1415.
21. Jespersen S, Niessen WMA, Tjaden UR, van der Greef J. Quantitative bioanalysis using matrix-assisted laser desorption/ionization mass spectrometry. J Mass Spectrom 1995;30:357-64.
22. Lavin AE, Merewether LA, Clogston CL, Rohde MF. Comparison of the high sensitivity and standard versions of Applied Biosystems 494 N-terminal protein sequencers using various sequencing supports. In (D.R. Marshak, ed.) Techniques in Protein Chemistry VIII, Academic Press, San Diego, pp. 57-67 (1997).