Fluorescence Detection of Heavy Atom Labeling (FD-HAL): A rapid method for identifying covalently modified cysteine residues by phasing atoms

Journal of Structural Biology

Volumn 171, Issue 1, 2010, Pages 82-87

  Chaptal, Vincent ^a   Ujwal, Rachna ^a   Nie, Yiling ^a   Watanabe, Akira ^a   Kwon, Seunghyug ^a   Abramson, Jeff ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cysteine accessibility; FD HAL; Heavy atom labeling; Membrane protein crystallography; Phasing atom detection; TMRM

Indexed keywords

CYSTEINE; GOLD DERIVATIVE; MALEIMIDE DERIVATIVE; MEMBRANE PROTEIN; MERCURY; PLATINUM; PROTEIN LACY; SODIUM GLUCOSE COTRANSPORTER; TETRAMETHYLRHODAMINE 5 MALEIMIDE; UNCLASSIFIED DRUG; VOLTAGE DEPENDENT ANION CHANNEL 1;

ARTICLE; ATOM; CHEMICAL MODIFICATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; COVALENT BOND; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; DERIVATIZATION; FLUORESCENCE DETECTION OF HEAVY ATOM LABELING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE;

ANIMALS; CHEMISTRY TECHNIQUES, ANALYTICAL; CRYSTALLOGRAPHY; CYSTEINE; FLUORESCENCE; GOLD; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MERCURY; MICE; MODELS, MOLECULAR; PLATINUM; SODIUM-GLUCOSE TRANSPORT PROTEINS; VOLTAGE-DEPENDENT ANION CHANNEL 1;

EID: 77953611152     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2010.02.005     Document Type: Article

References (28)

1. Abramoff M.D., Magelhaes P.J., Ram S.J. Image processing with ImageJ. Biophotonics Int. 2004, 11(7):36-42.
2. Abramson J., Smirnova I., Kasho V., Verner G., Kaback H.R., Iwata S. Structure and mechanism of the lactose permease of Escherichia coli. Science 2003, 301(5633):610-615.
3. Benz R. Porin from bacterial and mitochondrial outer membranes. CRC Crit. Rev. Biochem. 1985, 19:145-190.
4. Blake C.C. The preparation of isomorphous derivatives. Adv. Protein Chem. 1968, 23:59-120.
5. Boggon T.J., Shapiro L. Screening for phasing atoms in protein crystallography. Structure 2000, 8(7):R143-R149.
6. Carpenter E.P., Beis K., Cameron A.D., Iwata S. Overcoming the challenges of membrane protein crystallography. Curr. Opin. Struct. Biol. 2008, 18(5):581-586.
7. Carvin D., Islam S.A., Sternberg M.J.E., Blundell T.L. The preparation of heavy-atom derivatives of protein crystals for use in multiple isomorphous replacement and anomalous scattering. Int. Tables Crystallogr. 2006, F(Part 12.1):247-255.
8. Chayen N.E., Saridakis E. Protein crystallization: from purified protein to diffraction-quality crystals. Nat. Methods 2008, 5(2):147-153.
9. Cohen S.L., Padovan J.C., Chait B.T. Mass spectrometric analysis of mercury incorporation into proteins for X-ray diffraction phase determination. Anal. Chem. 2000, 72(3):574-579.
10. Drew D., Lerch M., Kunji E., Slotboom D.J., de Gier J.W. Optimization of membrane protein overexpression and purification using GFP fusions. Nat. Methods 2006, 3(4):303-313.
11. Drew D., Newstead S., Sonoda Y., Kim H., von Heijne G., Iwata S. GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae. Nat. Protoc. 2008, 3(5):784-798.
12. Faham S., Bowie J.U. Bicelle crystallization: a new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structure. J. Mol. Biol. 2002, 316(1):1-6.
13. Faham S., Watanabe A., Besserer G.M., Cascio D., Specht A., Hirayama B.A., Wright E.W., Abramson J. The crystal structure of a sodium galactose transporter reveals mechanistic insights into Na+/sugar symport. Science 2008, 321(5890):810-814.
14. Frillingos S., Sahin-Tóth M., Wu J., Kaback H.R. Cys-scanning mutagenesis: a novel approach to structure function relationships in polytopic membrane proteins. FASEB J. 1998, 12(13):1281-1299.
15. Guan L., Kaback H.R. Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins. Nat. Protoc. 2007, 2(8):2012-2017.
16. Johansson L.C., Wöhri A.B., Katona G., Engström S., Neutze R. Membrane protein crystallization from lipidic phases. Curr. Opin. Struct. Biol. 2009, 19(4):372-378.
17. Kawate T., Gouaux E. Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins. Structure 2006, 14(4):673-681.
18. Nie Y., Sabetfard F.E., Kaback H.R. The Cys154→Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway. J. Mol. Biol. 2008, 379(4):695-703.
19. Petsko G.A. Preparation of isomorphous heavy-atom derivatives. Methods Enzymol. 1985, 114:147-156.
20. Pompella A., Dominici S., Cambiaggi C., Frank J., Biesalski H.K. Cytofluorescence techniques for the visualization of distinct pools of protein thiols at the single cell level. Methods Mol. Biol. 2002, 196:305-312.
21. Stecher, P.G., Windholz, M., Leahy, D.S., Bolton, D.M., Eaton, L.G., 1968. The MERCK Index. An Encyclopedia of Chemicals and Drugs, eighth ed., Merck & Co. Inc., USA.
22. Sun P.D., Hammer C.H. Mass-spectrometry assisted heavy-atom derivative screening of human Fc gamma RIII crystals. Acta Crystallogr. D Biol. Crystallogr. 2000, 56(Pt 2):161-168.
23. Ujwal R., Cascio D., Colletier J.P., Faham S., Zhang J., Toro L., Ping P., Abramson J. The crystal structure of mouse VDAC1 at 2.3Å resolution reveals mechanistic insights into metabolite gating. Proc. Natl. Acad. Sci. USA 2008, 105(46):17742-17747.
24. Villa S., De Fazio G., Canosi U. Cyanogen bromide cleavage at methionine residues of polypeptides containing disulfide bonds. Anal. biochem. 1989, 177:161-164.
25. Wagner S., Klepsh M.M., Schlegel S., Appel A., Draheim R., Tarry M., Hogbom M., van Wijk K.J., Slotboom D.J., Persson J.O., de Gier J.W. Tuning Escherichia coli for membrane protein expression. Proc. Natl. Acad. Sci. USA 2008, 105(38):14371-14376.
26. White S.H. Biophysical dissection of membrane proteins. Nature 2009, 459(7245):344-346.
27. Wittig I., Schägger H. Features and applications of blue-native and clear-native electrophoresis. Proteomics 2008, 8(19):3974-3990.
28. Wright E.W., Turk E. The sodium/glucose cotransport family SLC5. Pflugers Arch. 2004, 447(5):510-518.