Effects of cell-seeding methods of human osteoblast culture on biomechanical properties of porous bioceramic scaffold

Biotechnology and Bioprocess Engineering

Volumn 15, Issue 2, 2010, Pages 341-348

  Jung, Duk Young ^a,b   Yamada, Takashi ^c   Tsuchiya, Toshie ^c   Ryu, Su Chak ^d   Han, Dong Wook ^d  

^a Senior Products Industrial Center   (South Korea)

^b Korea University Ansan Hospital   (South Korea)

^c NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

^d Pusan National University   (South Korea)

Author keywords

Cell seeding method; Human osteoblasts; Mechanical property; Porous tricalcium phosphate scaffold; Vacuum

Indexed keywords

HUMAN OSTEOBLAST; SEEDING METHOD; TRI-CALCIUM PHOSPHATES;

ANIMAL CELL CULTURE; BIOMATERIALS; BIOMECHANICS; CELL CULTURE; CELL PROLIFERATION; CENTRIFUGATION; CERAMIC MATERIALS; COMPRESSIVE STRENGTH; MECHANICAL PROPERTIES; OSTEOBLASTS; SEED; VACUUM; WETTING;

SCAFFOLDS;

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; BIOCERAMICS; CALCIUM PHOSPHATE; POROUS POLYMER;

ARTICLE; BIOMECHANICS; CELL CULTURE; CELL DENSITY; CELL PROLIFERATION; CELL SEEDING; COCULTURE; CONTROLLED STUDY; HUMAN; HUMAN CELL; INCUBATION TIME; OSTEOBLAST; PROTEIN FOLDING;

EID: 77953581676     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12257-009-0221-x     Document Type: Article

References (24)

1. Burg, K, J., S. Porter, and J. F. Kellam (2000) Biomaterial developments for bone tissue engineering. Biomaterials 21: 2347-2359.
2. Sikavitsas, V. I., J. S. Temenoff, and A. G. Mikos (2001) Biomaterials and bone mechanotransduction. Biomaterials 22: 2581-2593.
3. Cong, Z., W. Jianxin, F. Huaizhi, L. Bing, and Z. Zingdong (2001) Repairing segmental bone defects with living porous ceramic cylinders: an experimental study in dog femora. J. Biomed. Mater. Res. 55: 28-32.
4. Hing, K. A., B. Annaz, S. Saeed, A. Revell, and T. Buckland (2005) Microporosity enhances bioactivity of synthetic bone graft substitutes. J. Mater. Sci. Mater. Med. 16: 467-475.
5. Woodard, J. R., A. J. Hilldore, S. K. Lan, C. J. Park, A. W. Morgan, J. A. Eurell, S. G. Clark, M. B. Wheeler, R. D. Jamison, and A. J. Wagoner Johnson (2007) The mechanical properties and ostoconductivity of hydroxyapatite bone scaffolds with multi-scale porosity. Biomaterials 28: 45-54.
6. Vunjak-Novakovic, G., B. Obradovic, I. Martin, P. M. Bursac, R. Langer, and L. E. Freed (1998) Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering. Biotechnol. Prog. 14: 193-202.
7. Yang, T. H., H. Miyoshi, and N. Ohshima (2001) Novel cell immobilization method utilizing centrifugal force to achieve high-density hepatocyte culture in porous scaffold. J. Biomed. Mater. Res. 55: 379-386.
8. Yang, Y., J. L. Magnay, L. Cooling, and A. J. El Haj (2002) Development of a 'mechano-active' scaffold for tissue engineering. Biomaterials 23: 2119-2126.
9. Dong, J., T. Uemura, H. Kojima, M. Kikuchi, J. Tanaka, and T. Tateishi (2001) Application of low-pressure system to sustain in vivo bone formation in osteoblast/porous hydroxyapatite composite. Mater. Sci. Eng. C 17: 37-43.
10. Dong, J., T. Uemura, M. Kikuchi, J. Tanaka, and T. Tateishi (2002) Long-term durability of porous hydoxyapatite with lowpressure system to support osteogenesis of mesenchymal stem cells. Biomed. Mater. Eng. 12: 203-209.
11. Uemura, T., J. Dong, Y. Wang, H. Kojima, T. Saito, D. Iejima, M. Kikuchi, J. Tanaka, and T. Takeishi (2003) Transplantation of cultured bone cells using combinations of scaffolds and culture techniques. Biomaterials 24: 2277-2286.
12. Vuola, J., R. Taurio, H. Goransson, and S. Asko-Seljavaara (1998) Compressive strength of calcium carbonate and hydroxyapatite implants after bone-marrow-induced osteogenesis. Biomaterials 19: 223-227.
13. Tamai, N., A. Myoui, T. Tomita, T. Nakase, J. Tanaka, T. Ochi, and H. Yoshikawa (2002) Novel hydoxyapatite ceramics with an interconnective porous structure exhibit superior ostoconduction in vivo. J. Biomed. Mater. Res. 59: 110-117.
14. Xu, H. H. and C. G. Simon, Jr. (2005) Fast setting calcium phosphate-chitosan scaffold: Mechanical properties and biocompatibility. Biomaterials 26: 1337-1348.
15. Solchaga, L. A., E. Tognana, K. Penick, H. Baskaran, V. M. Goldberg, A. I. Caplan, and J. F. Welter (2006) A rapid seeding technique for the assembly of large cell/scaffold composite constructs. Tissue Eng. 12: 1851-1863.
16. Mastrogiacomo, M., S. Scaglione, R. Martinetti, L. Dolcini, F. Beltrame, R. Cancedda, and R. Quarto (2006) Role of scaffold internal structure on in vivo bone formation in macroporous calcium phosphate bioceramics. Biomaterials 27: 3230-3237.
17. Grodie, J. C., J. Merry, and M. H. Grant (2006) The mechanical properties of calcium phosphate ceramic modified by collagen coating and populated by osteoblasts. J. Mater. Sci. Mater. Med. 17: 43-48.
18. Rabiee, S. M., S. M. J. Mortazavi, F. Moztarzadeh, D. Sharifi, F. Fakhrejahani, A. Khafaf, S. A. Houshiar Ahmadi, N. Nosoudi, and R. Ravarian (2009) Association of a synthetic bone graft and bone marrow cells as a composite biomaterial. Biotechnol. Bioprocess Eng. 14: 1-5.
19. Albrektsson, T. and C. Johansson (2001) Osteoinduction, osteoconduction and osseointegration. Eur. Spine J. 10: S96-S101.
20. Guo, H., J. Su, J. Wei, H. Kong, and C. Liu (2009) Biocompatibility and osteogenicity of degradable Ca-deficient hydroxyapatite scaffolds from calcium phosphate cement for bone tissue engineering. Acta Biomaterialia 5: 268-278.
21. Kasten, P., I. Beyen, P. Niemeyer, R. Luginbühl, M. Bohner, and W. Richter (2008) Porosity and pore size of β-tricalcium phosphate scaffold can influence protein production and osteogenic differentiation of human mesenchymal stem cells: an in vitro and in vivo study. Acta Biomaterialia 4: 1904-1915.
22. Jo, I., J. M. Lee, H. Suh, and H. Kim (2007) Bone tissue engineering using marrow stromal cells. Biotechnol. Bioprocess Eng. 12: 48-53.
23. Dvir-Ginzberg, M., I. Gamlieli-Bonshtein, R. Agbaria, and S. Cohen (2003) Liver tissue engineering within alginate scaffolds: effects of cell-seeding density on hepatocyte viability, morphology, and function. Tissue Eng. 9: 757-766.
24. Choi, K.-M. Choi, Y.-K. Seo, H.-H. Yoon, K.-Y. Song, S.-Y. Kwon, H.-S. Lee, and J.-K. Park (2007) Effects of mechanical stimulation on the proliferation of bone marrow-derived human mesenchymal stem cells. Biotechnol. Bioprocess Eng. 12: 601-609.