Laser microdissection microscopy and single cell PCR of avian hemosporidians

Journal of Parasitology

Volumn 96, Issue 2, 2010, Pages 420-424

  Palinauskas, Vaidas ^a   Dolnik, Olga V ^a,b   Valkiunas, Gediminas ^a   Bensch, Staffan ^a,c  

^a VILNIUS UNIVERSITY   (Lithuania)

^b UNIVERSITY OF KIEL   (Germany)

^c LUND UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CHELATING AGENT; CHELEX; CYTOCHROME B; DNA; PROTEINASE K; UNCLASSIFIED DRUG;

BIRD; CYTOCHROME; FISH; GENETIC ANALYSIS; GENETIC MARKER; HEMOPARASITE; INFECTIOUS DISEASE; MICROSCOPY; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION; PROTOZOAN; REPTILE; SPECIES DIVERSITY; SPECIES RICHNESS;

ANIMAL CELL; ARTICLE; AVIAN MALARIA; BIRD; CONTROLLED STUDY; CYTOCHROME B GENE; DNA ISOLATION; DNA SEQUENCE; FRINGILLA COELEBS; GAMETOCYTE; GENE; GENE AMPLIFICATION; GENETIC MARKER; HAEMOPROTEUS MAJORIS; HAEMOPROTEUS TARTAKOVSKYI; HAEMOSPORIDA; LASER MICRODISSECTION; MICROSCOPY; NONHUMAN; NUCLEOTIDE SEQUENCE; OOKINETE; PLASMODIUM; PLASMODIUM RELICTUM; POLYMERASE CHAIN REACTION; SCHIZONT; SENSITIVITY ANALYSIS; SPINUS SPINUS; ZYGOTE;

ANIMALS; BIRD DISEASES; CHELATING AGENTS; DNA, PROTOZOAN; GENETIC MARKERS; HAEMOSPORIDA; MALARIA, AVIAN; MICRODISSECTION; MICROSCOPY, CONFOCAL; PASSERIFORMES; PLASMODIUM; POLYMERASE CHAIN REACTION; POLYSTYRENES; POLYVINYLS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA;

AVES; HAEMOPROTEUS; REPTILIA;

EID: 77953552210     PISSN: 00223395     EISSN: None     Source Type: Journal    
DOI: 10.1645/GE-2247.1     Document Type: Article

References (38)

1. ANDERSON, T. J. C., B. HAUBOLD, AND J. T. WILLIAMS. 2000. Microsatellite markers reveal a spectrum of population structure in the malaria parasite Plasmodium falciparum. Molecular Biology and Evolution 17: 1467-1482.
2. BARKER, D. L., M. S. T. HANSEN, AND A. F. FARUQI. 2004. Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel. Genome Research 14: 901-907.
3. BENSCH, S., O. HELLGREN, AND J. PÉ REZ-TRIS. 2009. MalAvi: A public database of malaria parasites and related haemosporidians in avian hosts based on mitochondrial cytochrome b lineages. Molecular Ecology Resources 9: 1353-1358.
4. J. PÉREZ-TRIS, J. WALDENSTRÖM, O. HELLGREN. 2004. Linkage between nuclear and mitochondrial DNA sequences in avian malaria parasites-Multiple cases of cryptic speciation? Evolution 58 1617-1621.
5. -, M. STJERNMAN, AND D. HASSELQUIST. 2000. Host specificity in avian blood parasites: A study of Plasmodium and Haemoproteus mitochondrial DNA amplified from birds. Proceedings of the Royal Society of London Series B, Biological Sciences 267: 1583-1589.
6. -, J. WALDENSTRÖM, AND N. JONZÉN. 2007. Temporal dynamics and diversity of avian malaria parasites in a single host species. Journal of Animal Ecology 76: 112-122.
7. DOLEŽEL, J., J. BARTOS, H. VOGLMAYR, AND J. GREILHUBER. 2003. Nuclear DNA content and genome size of trout and human. Cytometry Part A 51: 127-128.
8. DOLNIK, O. V., V. PALINAUSKAS, AND S. BENSCH. 2009. Individual oocysts of Isospora (Apicomplexa: Coccidia) parasites from avian feces: From photo to sequence. Journal of Parasitology 95: 169-174.
9. EDOH, D., S. STEIGER, B. GENTON, AND H. P. BECK. 1997. PCR amplification of DNA from malaria parasites on fixed and stained thick and thin blood films. Transactions of the Royal Society of Tropical Medicine and Hygiene 91: 361-363.
10. ELLEGREN, H. 2008. Sequencing goes 454 and takes large-scale genomics into the wild. Molecular Ecology 17: 1629-1635.
11. FELDMAN, R. A., L. A. FREED, AND R. L. CANN. 1995. A PCR test for avian malaria in Hawaiian birds. Molecular Ecology 4: 663-673.
12. FREED, L. A., AND R. L. CANN. 2006. DNA quality and accuracy of avian malaria PCR diagnostics: A review. Condor 108: 459-473.
13. GODFREY, R. D., A. M. FEDYNICH, AND D. B. PENCE. 1987. Quantification of haematozoa in blood smears. Journal of Wildlife Diseases 23: 558-565.
14. GRAZYK, T. K., M. R. CRANFIELD, AND C. J. SHIFF. 1994. Extraction of Haemoproteus columbae (Haemosporina: Haemoproteidae) antigen from rock dove pigeons (Columba livia) and its use in an antibody ELISA. Journal of Parasitology 80: 713-718.
15. HALL, T. A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows95/98/NT. Nucleic Acids Symposium Series 41: 95-98.
16. HELLGREN, O., J. WALDENSTRÖM, AND S. BENSCH. 2004. A new PCR assay for simultaneous studies of Leucocytozoon, Plasmodium, and Haemoproteus from avian blood. Journal of Parasitology 90: 797-802.
17. JONES, M. K., L. M. RANDALL, D. P. MCMANUS, AND C. R. ENGWERDA. 2004. Laser microdissection microscopy in parasitology: Microscopes meet thermocyclers. Trends in Parasitology 20: 502-506.
18. KIMURA, M., O. KANEKO, A. INOUE, A. ISHII, AND K. TANABE. 1995. Amplification by polymerase chain reaction of Plasmodium falciparum DNA from Giemsa-stained thin blood smears. Molecular and Biochemical Parasitology 70: 193-197.
19. MARTINSEN, E. S., S. L. PERKINS, AND J. J. SCHALL. 2008. A three-genome phylogeny of malaria parasites (Plasmodium and closely related genera): Evolution of life-history traits and host switches. Molecular Phylogenetics and Evolution 47: 261-273.
20. PAIN, A., AND C. HERTZ-FOWLER. 2009. Plasmodium genomics: Latest milestone. Nature Reviews Microbiology 7: 180-181.
21. PALINAUSKAS, V., G. VALKIUμNAS, V. C. BOLSHAKOV, AND S. BENSCH. 2008. Effects of Plasmodium relictum (lineage P-SGS1) on experimentally infected passerine birds. Experimental Parasitology 120: 372-380.
22. PÉ REZ-TRIS, J., AND S. BENSCH. 2005. Dispersal increases local transmission of avian malarial parasites. Ecology Letters 8: 838-845.
23. -, O. HELLGREN, AND A. KRIŽANAUSKIENĖ. 2007. Within-host speciation of malaria parasites. PloS ONE 2: e235Links.
24. PERKINS, S. L., AND J. J. SCHALL. 2002. A molecular phylogeny of malarial parasites recovered from cytochrome b gene sequences. Journal of Parasitology 88: 972-978.
25. POLJAK, M., K. SENE, AND N. GALE. 2000. Rapid extraction of DNA from archival clinical specimens: Our experiences. Pflügers Archiv European Journal of Physiology 439: 42-44.
26. RAZAKANDRAINIBE, F. G., P. DURAND, AND J. C. KOELLA. 2005. "Clonal" population structure of the malaria agent Plasmodium falciparum in high-infection regions. Proceedings of the National Academy of Sciences of the United States of America 102: 17388-17393.
27. RICHARDSON, D. S., F. L. JURY, K. BLAAKMEER, J. KOMDEUR, AND T. BURKE. 2001. Parentage assignment and extra-group paternity in a cooperative breeder: The Seychelles warbler (Acrocephalus sechellensis). Molecular Ecology 10: 2263-2273.
28. RICKLEFS, R. E., AND S. M. FALLON. 2002. Diversification and host switching in avian malaria parasites. Proceedings of the Royal Society of London Series B, Biological Sciences 269: 885-892.
29. SACCI, J. B., J. C. AGUIAR, A. O. LAU, AND S. L. HOFFMAN. 2002. Laser capture microdissection and molecular analysis of Plasmodium yoelli liver-stage parasites. Molecular and Biochemical Parasitology 119: 285-289.
30. SAMBROOK, J., E. F. FRITSCH, AND T. MANIATIS. 1989. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 720 p.
31. SCOPEL, K. K. G., C. J. F. FONTES, A. C. NUNES, M. DE FÁ TIMA HORTA, AND E. M. BRAGA. 2004. Low sensitivity of nested PCR using Plasmodium DNA extracted from stained thick blood smears: An epidemiological retrospective study among subjects with low parasitemia in an endemic area of the Brazilian Amazon region. Malaria Journal 3: 8.
32. SEMBLAT, J. P., O. SILVIE, AND J. F. FRANETICH. 2002. Laser capture microdissection of Plasmodium falciparum liver stages for mRNA analysis. Molecular and Biochemical Parasitology 121: 179-183.
33. VALKIUμNAS, G. 2005. Avian malaria parasites and other haemosporidia. CRC Press, Boca Raton, Florida, 946 p.
34. -, C. T. ATKINSON, S. BENSCH, R. N. M. SEHGAL, AND R. E. RICKLEFS. 2008. Parasite misidentifications in GenBank: How to minimise their number? Trends in Parasitology 24: 247-248.
35. -, S. BENSCH, AND T. A. IEZHOVA. 2006. Nested cytochrome b polymerase chain reaction diagnostics underestimate mixed infections of avian blood haemosporidian parasites: Microscopy is still essential. Journal of Parasitology 92: 418-422.
36. -, T. A. IEZHOVA, A. KRIŽANAUSKIENĖ , V. PALINAUSKAS, AND S. BENSCH. 2008. In vitro hybridization of Haemoproteus spp.: An experimental approach for direct investigation of reproductive isolation of parasites. Journal of Parasitology 94: 1385-1394.
37. WALSH, P. S., D. A. METZGER, AND R. HIGUCHI. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechnics 10: 506-513.
38. ZHANG, L., X. CUI, AND K. SCHMITT. 1992. Whole genome amplification from a single cell: Implications for genetic analysis. Proceedings of the National Academy of Sciences of the United States of America 89: 5847-5851.