Non-viral VEGF165 gene therapy - magnetofection of acoustically active magnetic lipospheres ('magnetobubbles') increases tissue survival in an oversized skin flap model

Journal of Cellular and Molecular Medicine

Volumn 14, Issue 3, 2010, Pages 587-599

  Holzbach, Thomas ^a   Vlaskou, Dialekti ^b   Neshkova, Iva ^a   Konerding, Moritz A ^c   Wörtler, Klaus ^a   Mykhaylyk, Olga ^b   Gänsbacher, Bernd ^b   Machens, H G ^a   Plank, Christian ^b   Giunta, Riccardo E ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b Institute of Experimental Genetics   (Germany)

^c JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Angiogenesis; Gene therapy; Lipospheres; Magnetic; Magnetobubbles; Magnetofection; VEGF

Indexed keywords

GLYCINE MAX; RATTUS;

LIPID; MICROSPHERE; VASCULOTROPIN;

ANIMAL; ANIMAL MODEL; ARTICLE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE THERAPY; GENETIC TRANSFECTION; GENETICS; GRAFT SURVIVAL; MAGNETISM; MALE; METABOLISM; METHODOLOGY; MICROVASCULATURE; MUSCLE; PHYSIOLOGY; PLASTIC SURGERY; RAT; SKIN; SKIN TRANSPLANTATION; SPRAGUE DAWLEY RAT; ULTRASOUND; VASCULARIZATION;

ANIMALS; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE THERAPY; GRAFT SURVIVAL; LIPIDS; MAGNETICS; MALE; MICROSPHERES; MICROVESSELS; MODELS, ANIMAL; MUSCLES; RATS; RATS, SPRAGUE-DAWLEY; SKIN; SKIN TRANSPLANTATION; SURGICAL FLAPS; TRANSFECTION; ULTRASONICS; VASCULAR ENDOTHELIAL GROWTH FACTORS;

EID: 77953527655     PISSN: 15821838     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1582-4934.2008.00592.x     Document Type: Article

References (29)

1. Giunta RE, Holzbach T, Taskov C. AdVEGF165 gene transfer increases survival in overdimensioned skin flaps. J Gene Med. 2005, 7:297-306.
2. Laitinen M, Makinen K, Manninen H. Adenovirus-mediated gene transfer to lower limb artery of patients with chronic critical leg ischemia. Hum Gene Ther. 1998, 9:1481-6.
3. Laitinen M, Hartikainen J, Hiltunen MO. Catheter-mediated vascular endothelial growth factor gene transfer to human coronary arteries after angioplasty. Hum Gene Ther. 2000, 11:263-70.
4. Mäkinen K, Manninen H, Hedman M. Increased vascularity detected by digital subtraction angiography after VEGF gene transfer to human lower limb artery: a randomized, placebo-controlled, double-blinded phase II study. Mol Ther. 2002, 6:127-33.
5. Mann MJ, Whittemore AD, Donaldson MC. Ex-vivo gene therapy of human vascular bypass grafts with E2F decoy: the PREVENT single-centre, randomised, controlled trial. Lancet. 1999, 354:1493-8.
6. Grines CL, Watkins MW, Helmer G. Angiogenic Gene Therapy (AGENT) trial in patients with stable angina pectoris. Circulation. 2002, 105:1291-7.
7. Sylvén C, Sarkar N, Ruck A. Myocardial Doppler tissue velocity improves following myocardial gene therapy with VEGF-A165 plasmid in patients with inoperable angina pectoris. Coron Artery Dis. 2001, 12:239-43.
8. Kutryk MJ, Foley DP, van den Brand M. Local intracoronary administration of antisense oligonucleotide against c-myc for the prevention of in-stent restenosis: results of the randomized investigation by the Thoraxcenter of antisense DNA using local delivery and IVUS after coronary stenting (ITALICS) trial. J Am Coll Cardiol. 2002, 39:281-7.
9. Rosengart TK, Lee LY, Patel SR. Angiogenesis gene therapy: phase I assessment of direct intramyocardial administration of an adenovirus vector expressing VEGF121 cDNA to individuals with clinically significant severe coronary artery disease. Circulation. 1999, 100:468-74.
10. Scherer F, Anton M, Schillinger U. Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo. Gene Ther. 2002, 9:102-9.
11. Luo D, Saltzman WM. Enhancement of transfection by physical concentration of DNA at the cell surface. Nat Biotechnol. 2000, 18:893-5.
12. Gersting SW, Schillinger U, Lausier J. Gene delivery to respiratory epithelial cells by magnetofection. J Gene Med. 2004, 6:913-22.
13. Unger EC, McCreery TP, Sweitzer RH. Acoustically active lipospheres containing paclitaxel: a new therapeutic ultrasound contrast agent. Invest Radiol. 1998, 33:886-92.
14. Vlaskou D, Mykhaylyk O, Giunta RE. Magnetic microbubbles: new carriers for localized gene and drug delivery. Molecular Therapy 2006, 13:290.
15. Liu Y, Miyoshi H, Nakamura M. Encapsulated ultrasound microbubbles: therapeutic application in drug/gene delivery. J Control Release. 2006, 114:89-99.
16. Giunta RE, Holzbach T, Taskov C. Prediction of flap necrosis with laser induced indocyanine green fluorescence in a rat model. Br J Plast Surg. 2005, 58:695-701.
17. Holzbach T, Taskov C, Henke J. Evaluation der Perfusion von Lappenplastiken mittels Laserfluoreszenz von Indocyaningrün. Handchir Mikrochir Plast Chir. 2005, 37:396-402.
18. Mykhaylyk O, Antequera YS, Vlaskou D. Generation of magnetic nonviral gene transfer agents and magnetofection in vitro. Nature protocols. 2007, 2:2391-411.
19. Milton SH. Pedicled skin-flaps: the fallacy of the length: width ratio. Br J Surg. 1970, 57:502-8.
20. Holzbach T, Neshkova I, Vlaskou D. Searching for the right timing of surgical delay: angiogenesis, vascular endothelial growth factor and perfusion changes in a skin-flap model. J Plast Reconstr Aesthet Surg. 2009, 62:1534-42.
21. Chalkley HW. Method for the quantitative morphologic analysis of tissues. J Natl Cancer Inst. 1943, 4:47-53.
22. Li C, Gardy R, Seon BK. Both high intratumoral microvessel density determined using CD105 antibody and elevated plasma levels of CD105 in colorectal cancer patients correlate with poor prognosis. Br J Cancer. 2003, 88:1424-31.
23. Konerding MA, Fait E, Gaumann A. 3D microvascular architecture of pre-cancerous lesions and invasive carcinomas of the colon. Br J Cancer. 2001, 84:1354-62.
24. Ravnic DJ, Jiang X, Wolloscheck T. Vessel painting of the microcirculation using fluorescent lipophilic tracers. Microvasc Res. 2005, 70:90-6.
25. Marcus R, Peritz E, Gabriel KR. On closed testing procedures with special reference to ordered analysis of variance. Biometrika. 1976, 63:655-60.
26. O'Toole G, MacKenzie D, Lindeman R. Vascular endothelial growth factor gene therapy in ischaemic rat skin flaps. Br J Plast Surg. 2002, 55:55-8.
27. Huth S, Lausier J, Gersting SW. Insights into the mechanism of magnetofection using PEI-based magnetofectins for gene transfer. J Gene Med. 2004, 6:923-36.
28. Noble JG, Lee V, Griffith-Noble F. Therapeutic ultrasound: the effects upon cutaneous blood flow in humans. Ultrasound Med Biol. 2007, 33:279-85.
29. Newman CM, Bettinger T. Gene therapy progress and prospects: ultrasound for gene transfer. Gene Ther. 2007, 14:465-75.