Detection of Clostridium sp. and its Relation to different ages and gastrointestinal segments as Measured by molecular analysis of 16S rRNA genes

Brazilian Archives of Biology and Technology

Volumn 53, Issue 1, 2010, Pages 69-76

  Mirhosseini, Seyed Ziaeddin ^a,b   Seidavi, Alireza ^c   Shivazad, Mahmoud ^d   Chamani, Mohammad ^e   Sadeghi, Ali Asghar ^e   Pourseify, Reza ^b  

^a UNIVERSITY OF GUILAN   (Iran)

^b Agriculture Biotechnology Research Institute of North Region of IRAN   (Iran)

^c ISLAMIC AZAD UNIVERSITY   (Iran)

^d UNIVERSITY OF TEHRAN   (Iran)

^e ISLAMIC AZAD UNIVERSITY   (Iran)

Author keywords

16S rRNA genes; Age; Broiler; Clostridium spp.; Gastrointestinal

Indexed keywords

CLOSTRIDIUM; CLOSTRIDIUM SP.; GALLUS GALLUS;

EID: 77953355070     PISSN: 15168913     EISSN: 15168913     Source Type: Journal    
DOI: 10.1590/S1516-89132010000100009     Document Type: Article

References (22)

1. Amit-Romach, E.; Sklan, D. and Uni, Z. (2004), Microflora Ecology of the Chicken Intestine Using 16S Ribosomal DNA Primers. Poult. Sci. 83, 1093-1098.
2. Apajalahti, J.H.; Sarkilahti, L.K.; Maki, B.R.; Heikkinen, J.P.; Nurminen, P.H. and Holben, W.E. (1998), Effective recovery of bacterial DNA and percent guanine-plus-cytosine-based analysis of community structure in the gastrointestinal tract of broiler chickens. Appl Environ Microbiol. 64, 4084-4088.
3. Barbara, A.J.; Trinh, H.T.; Glock, R.D. and Songer, J.G. (2007), Necrotic enteritis-producing strains of Clostridium perfringens displace nonnecrotic enteritis strains from the gut of chicks. Veterinary Microbiology (In Press). doi:10.1016/j. vetmic. 2007.07.019.
4. Carlin, F.; Broussolle, V.; Perelle, S.; Litman, S. and Fach, P. (2004),. Prevalence of Clostridium botulinum in food raw materials used in REPFEDs manufactured in France. Int. J. Food Microbiol. 91, 141-145.
5. Csordas, A. (1995), Toxicology of butyrate and shortchain fatty acids. In: Role of Gut Bacteria in Human Toxicology and Pharmacology (Hill, M.J., Ed.), pp. 105-125. Taylor and Francis, London.
6. Engström, B.E.; Fermér, C.; Lindberg, A.; Saarinen, E.; Båverud, V. and Gunnarsson, A. (2003), Molecular typing of isolates of Clostridium perfringens from healthy and diseased poultry. Vet. Microbiol. 94, 225-235.
7. Ficken, M.D. and Wages, D.P. (1997), Necrotic enteritis. In: Barnes, H.J.; Beard, C.W.; McDougald, L.; Saif, Y.M. and Calnek, B.W. (Eds.), Diseases of Poultry. Iowa State University press, Ames, IA, pp. 261-264.
8. Gholamiandekhordi, A.R.; Ducatelle, R.; Heyndrickx, M.; Haesebrouck, F. and Van-Immerseel, P. (2006), Molecular and phenotypical characterization of Clostridium perfringens isolates from poultry flocks with different disease status. Vet. Microbiol. 113, 143-152.
9. Gong, J.; Forster, R.J.; Yu, H.; Chambers, J.R.; Wheatcroft, R.; Sabour, P.M. and Chen, S. (2002), Molecular analysis of bacterial populations in the ileum of broiler chickens and comparison with bacteria in the cecum. FEMS Microbiol. Ecol. 41, 171-179.
10. Grave, K.; Kaldhusdal, M.; Kruse, H.; FevangHarr, L.M. and Flatlandsmo, K. (2004), What has happened in Norway after the ban of Avoparcin? Consumption of antimicrobials by poultry. Preventive Vet. Med. 62, 59-72.
11. Harris, I.T.; Fedorka-Caray, P.J.; Gray, J.T.; Thomas, L.A. and Ferris, K. (1997), Prevalence of Salmonella organisms in swine feed. J. American Vet. Med. Assoc. 1210(3), 382-385.
12. Hill, J.E.; Seipp, R.P.; Betts, M.; Hawkins, L.; Van Kessel, A.G. and Crosby, W.L. (2002), Extensive profiling of a complex microbial community by highthroughput sequencing. Appl. Environ. Microbiol. 68, 3055-3066.
13. Jaimes, C.P.; Aristizábal, F.A.; Bernal, M.; Suárez, Z.R. and Montoya, D. (2006), AFLP fingerprinting of Colombian Clostridium spp. strains, multivariate data analysis and its taxonomical implications. J. Microbiol. Methods. 67, 64-69.
14. Johansson, A.; Greko, C.; Engström, B.E. and Karlsson, M. (2004), Antimicrobial susceptibility of Swedish, Norwegian and Danish isolates of Clostridium perfringens from poultry, and distribution of tetracycline resistance genes. Vet. Microbiol. 99, 251-257.
15. Kaldhusdal, M.; Schneitz, C.; Hofshagen, M. and Skjerve, E. (2001), Reduced incidence of Clostridium perfringens-associated lesions and improved performance in broiler chickens treated with normal intestinal bacteria from adult fowl. Avian Dis. 45, 149-156.
16. La-Ragione, R.M. and Woodward, M.J. (2003), Competitive exclusion by Bacillus subtilis spores of Salmonella enterica serotype Enteritidis and Clostridium perfringens in young chickens. Vet. Microbiol. 94, 245-256.
17. Lu, J.; Domingo, J.S. and Shanks, O.C. (2007), Identification of chicken-specific fecal microbial sequences using a metagenomic approach. Water Res. 41, 3561-3574.
18. Lu, J.; Idris; J.; Harmon, B.G.; Hofacre, C.; Maurer, J.J. and Lee, M.D. (2003), Diversity and succession of the intestinal bacterial community of the maturing broiler chicken. Appl. Environ. Microbiol. 69, 6816-6824.
19. McCourt, M.T.; Finlay, D.A.; Laird, C.; Smyth, J.A.; Bell, C. and Ball, H.J. (2005); Sandwich ELISA detection of Clostridium perfringens cells and α-toxin from field cases of necrotic enteritis of poultry. Vet. Microbiol. 106, 259-264.
20. th ed. National Academy. Washington DC.
21. Nauerby, B.; Pedersen, K. and Madsen, M. (2003), Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens. Vet. Microbiol. 94, 257-266.
22. Olkowski, A.A.; Wojnarowicz, C.; Chirino-Trejo, M. and Drew, M.D. (2006), Responses of broiler chickens orally challenged with Clostridium perfringens isolated from field cases of necrotic enteritis. Res in Vet Sci. 81, 99-108.