Resistance of human brain microvascular endothelial cells in culture to methylmercury: Cell-density-dependent defense mechanisms

Journal of Toxicological Sciences

Volumn 35, Issue 3, 2010, Pages 287-294

  Hirooka, Takashi ^a   Fujiwara, Yasuyuki ^b   Shinkai, Yasuhiro ^a   Yamamoto, Chika ^a   Yasutake, Akira ^c   Satoh, Masahiko ^b   Eto, Komyo ^d   Kaji, Toshiyuki ^a  

^a HOKURIKU UNIVERSITY   (Japan)

^b AICHI GAKUIN UNIVERSITY   (Japan)

^c NATIONAL INSTITUTE FOR MINAMATA DISEASE   (Japan)

^d Health and Nursing Facilities for the Aged   (Japan)

Author keywords

Endothelial cells; Glutathione; L type large neutral amino acid transporter; Metallothionein; Methylmercury

Indexed keywords

AMINO ACID; CARRIER PROTEINS AND BINDING PROTEINS; GLUTATHIONE; L TYPE AMINO ACID TRANSPORTER 1; METALLOTHIONEIN; METHYLMERCURY; UNCLASSIFIED DRUG; AMINO ACID TRANSPORTER; METHYLMERCURY DERIVATIVE;

ARTICLE; BRAIN CELL; BRAIN MICROVASCULAR ENDOTHELIAL CELL; CELL ACTIVITY; CELL DAMAGE; CELL DENSITY; CELL GROWTH; CELL LYSATE; CELL MEMBRANE RESISTANCE; CYTOTOXICITY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN TISSUE; INTRACELLULAR TRANSPORT; MICROENVIRONMENT; NEUROTOXICITY; PERICYTE; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING; BRAIN; CELL COUNT; CELL CULTURE; CYTOLOGY; DRUG EFFECTS; DRUG RESISTANCE; ENDOTHELIUM CELL; METABOLISM; PATHOLOGY;

BRAIN; CELL COUNT; CELLS, CULTURED; DRUG RESISTANCE; ENDOTHELIAL CELLS; GLUTATHIONE; HUMANS; LARGE NEUTRAL AMINO ACID-TRANSPORTER 1; METHYLMERCURY COMPOUNDS;

EID: 77953317270     PISSN: 03881350     EISSN: 18803989     Source Type: Journal    
DOI: 10.2131/jts.35.287     Document Type: Article

References (29)

1. Aschner, M., Conklin, D.R., Yao, C.P., Allen, J.W. and Tan, K.H. (1998): Induction of astrocyte metallothioneins (MTs) by zinc confers resistance against the acute cytotoxic effects of methylmercury on cell swelling, Na+ uptake, and K+ release. Brain Res., 813, 254-261.
2. Ballatori, N. and Clarkson, T.W. (1983): Biliary transport of glutathione and methylmercury. Am. J. Physiol., 244, G435-441.
3. Dutczak, W.J. and Ballatori, N. (1994): Transport of the glutathionemethylmercury complex across liver canalicular membranes on reduced glutathione carriers. J. Biol. Chem., 269, 9746-9751.
4. Eto, K. (1997): Pathology of Minamata disease. Toxicol. Pathol., 25, 614-623.
5. Eto, K., Yasutake, A., Kuwana, T., Korogi, Y., Akima, M., Shimozeki, T., Tokunaga, H. and Kaneko, Y. (2001): Methylmercury poisoning in common marmosets - a study of selective vulnerability within the cerebral cortex. Toxicol. Pathol., 29, 565-573.
6. Fujiyama, J., Hirayama, K. and Yasutake, A. (1994): Mechanism of methylmercury efflux from cultured astrocytes. Biochem. Pharmacol., 47, 1525-1530.
7. Fujiwara, Y., Kaji, T., Yamamoto, C., Sakamoto, M. and Kozuka, H. (1995): Stimulatory effect of lead on the proliferation of cultured vascular smooth-muscle cells. Toxicology, 98, 105-110.
8. Hirayama, K. (1980): Effect of amino acids on brain uptake of methylmercury. Toxicol. Appl. Pharmacol., 55, 318-323.
9. Hirooka, T., Fujiwara, Y., Yamamoto, C., Yasutake, A. and Kaji, T. (2007): Methylmercury retards the repair of wounded monolayer of human brain microvascular endothelial cells by inhibiting their proliferation without nonspecific cell damage. J. Health Sci., 53, 450-456. (Pubitemid 47196248)
10. Hirooka, T., Fujiwara, Y., Inoue, S., Shinkai, Y., Yamamoto, C., Satoh, M., Yasutake, A., Eto, K. and Kaji, T. (2009): Suppression of fibroblast growth factor-2 expression: possible mechanism underlying methylmercury-induced inhibition of the repair of wounded monolayers of cultured human brain microvascular endothelial cells. J. Toxicol. Sci., 34, 433-439.
11. Hirooka, T., Fujiwara, Y., Minami, Y., Ishii, A., Ishigooka, M., Shinkai, Y., Yamamoto, C., Satoh, M., Yasutake, A., Eto, K. and Kaji, T. (2010): Cell-density-dependent methylmercury susceptibility of cultured human brain microvascular pericytes. Toxicol. In Vitro, 24, 835-841.
12. Hirrlinger, J., Gutterer, J.M., Kussmaul, L., Hamprecht, B. and Dringen, R. (2000): Microglial cells in culture express a prominent glutathione system for the defense against reactive oxygen species. Dev. Neurosci., 22, 384-392.
13. Huang, C.S., Chang, L.S., Anderson, M.E. and Meister, A. (1993a): Catalytic and regulatory properties of the heavy subunit of rat kidney γ-glutamylcysteine synthetase. J. Biol. Chem., 268, 19675-19680.
14. Huang, C.S., Anderson, M.E. and Meister, A. (1993b): Amino acid sequence and function of the light subunit of rat kidney γ- glutamylcysteine synthetase. J. Biol. Chem., 268, 20578-20583.
15. Hughes, W.L. (1957): A physicochemical rationale for the biological activity of mercury and its compounds. Ann. N. Y. Acad. Sci., 65, 454-460.
16. Iencean, S.M. (2003): Brain edema - a new classification. Med. Hypotheses, 61, 106-109.
17. Kaji, T., Fujiwara, Y., Hoshino, M., Yamamoto, C., Sakamoto, M. and Kozuka, H. (1995): Inhibitory effect of lead on the proliferation of cultured vascular endothelial cells. Toxicology, 95, 87-92.
18. Kerper, L.E., Mokrzan, E.M., Clarkson, T.W. and Ballatori, N. (1996): Methylmercury efflux from brain capillary endothelial cells is modulated by intracellular glutathione but not ATP. Toxicol. Appl. Pharmacol., 141, 526-531.
19. Kido, Y., Tamai, I., Uchino, H., Suzuki, F., Sai, Y. and Tsuji, A. (2001): Molecular and functional identification of large neutral amino acid transporters LAT1 and LAT2 and their pharmacological relevance at the blood-brain barrier. J. Pharm. Pharmacol., 53, 497-503.
20. Kissane, J.M. and Robins, E. (1958): The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. J. Biol. Chem., 233, 184-188.
21. Marmarou, A. (2004): The pathophysiology of brain edema and elevated intracranial pressure. Cleve. Clin. J. Med., 71, S6-8.
22. Miura, K. and Clarkson, T.W. (1993): Reduced methylmercury accumulation in a methylmercury-resistant rat pheochromocytoma PC12 cell line. Toxicol. Appl. Pharmacol., 118, 39-45.
23. Pineda, M., Fernández, E., Torrents, D., Estévez, R., López, C., Camps, M., Lloberas, J., Zorzano, A. and Palacín, M. (1999): Identification of a membrane protein, LAT-2, that Co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids. J. Biol. Chem., 274, 19738-19744.
24. Sanfeliu, C., Sebastià, J., Cristòfol, R. and Rodríguez-Farré, E. (2003): Neurotoxicity of organomercurial compounds. Neurotox. Res., 5, 283-306.
25. Sato, M. and Kondoh, M. (2002): Recent studies on metallothionein: protection against toxicity of heavy metals and oxygen free radicals. Tohoku J. Exp. Med., 196, 9-22.
26. Shanker, G., Aschner, J.L., Syversen, T. and Aschner, M. (2004): Free radical formation in cerebral cortical astrocytes in culture induced by methylmercury. Brain Res. Mol. Brain Res., 128, 48-57.
27. Simmons-Willis, T.A., Koh, A.S., Clarkson, T.W. and Ballatori, N. (2002): Transport of a neurotoxicant by molecular mimicry: The methylmercury-L-cysteine complex is a substrate for human Ltype large neutral amino acid transporter (LAT) 1 and LAT2. Biochem. J., 367, 239-246.
28. Yanagida, O., Kanai, Y., Chairoungdua, A., Kim, D.K., Segawa, H., Nii, T., Cha, S.H., Mastsuo, H., Fukushima, J., Fukasawa, Y., Tani, Y., Taketani, Y., Uchino, H., Kim, J.Y., Inatomi, J., Okayasu, I., Miyamoto, K., Takeda, E., Goya, T. and Endou, H. (2001): Human L-type amino acid transporter 1 (LAT1): Characterization of function and expression in tumor cell lines. Biochim. Biophys. Acta, 1514, 291-302.
29. Yao, C.P., Allen, J.W., Mutkus, L.A., Xu, S.B., Tan, K.H. and Aschner, M. (2000): Foreign metallothionein-I expression by transient transfection in MT-I and MT-II null astrocytes confers increased protection against acute methylmercury cytotoxicity. Brain Res., 855, 32-38.