Effects of tensile strain and fluid flow on osteoarthritic human chondrocyte metabolism in vitro

Journal of Orthopaedic Research

Volumn 28, Issue 7, 2010, Pages 907-913

  Mawatari, Taro ^a   Lindsey, Derek P ^b   Harris, Alex H S ^b   Goodman, Stuart B ^b   Maloney, William J ^a   Smith, Robert L ^a,b  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VA PALO ALTO HEALTH CARE SYSTEM   (United States)

Author keywords

Fluid flow; Gene expression; Human articular chondrocytes; Mechanical loading; Nitric oxide; Osteoarthritis

Indexed keywords

AGGRECAN; COLLAGEN TYPE 2; INTERLEUKIN 6; MESSENGER RNA; NITRIC OXIDE; TRANSCRIPTION FACTOR SOX9;

ADULT; ARTICLE; CARTILAGE CELL; CELL CULTURE; CELL METABOLISM; CULTURE MEDIUM; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUID FLOW; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; MECHANICAL STRESS; OSTEOARTHRITIS; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; TENSILE STRENGTH;

AGGRECANS; CELLS, CULTURED; CHONDROCYTES; COLLAGEN TYPE II; ENERGY METABOLISM; GENE EXPRESSION; HUMANS; INTERLEUKIN-6; MODELS, BIOLOGICAL; NITRIC OXIDE; OSTEOARTHRITIS, KNEE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SOX9 TRANSCRIPTION FACTOR; STRESS, MECHANICAL; TENSILE STRENGTH; WEIGHT-BEARING;

EID: 77953201585     PISSN: 07360266     EISSN: 1554527X     Source Type: Journal    
DOI: 10.1002/jor.21085     Document Type: Article

References (48)

1. Arokoski JP, Jurvelin JS, Väätäinen U, et al. 2000. Normal and pathological adaptations of articular cartilage to joint loading. Scand J Med Sci Sports 10:186-198.
2. Buckwalter JA, Mankin HJ. 1998. Articular cartilage: degeneration and osteoarthritis, repair, regeneration, and transplantation. Instr Course Lect 47:487-504.
3. Andriacchi TP, Mündermann A, Smith RL, et al. 2004. A framework for the in vivo pathomechanics of osteoarthritis at the knee. Ann Biomed Eng 32:447-457.
4. Radin EL, Burr DB, Caterson B, et al. 1991. Mechanical determinants of osteoarthrosis. Semin. Arthritis Rheum 21 (Suppl 2):12-21.
5. Carter DR, Beaupré GS, Wong M, et al. 2004. The mechanobiology of articular cartilage development and degeneration. Clin Orthop Relat Res 427(Suppl):S69-S77.
6. Thambyah A, Broom N. 2007. On how degeneration influences load-bearing in the cartilage-bone system: a microstructural and micromechanical study. Osteoarthritis Cart 15:1410-1423.
7. Buckwalter JA, Martin JA, Brown TD. 2006. Perspectives on chondrocyte mechanobiology and osteoarthritis. Biorheology 43:603-609.
8. Griffin TM, Guilak F. 2005. The role of mechanical loading in the onset and progression of osteoarthritis. Exerc Sport Sci Rev 33:195-200.
9. Beecher BR, Martin JA, Pedersen DR, et al. 2007. Antioxidants block cyclic loading induced chondrocyte death. Iowa Orthop J 27:1-8.
10. Martin JA, Buckwalter JA. 2006. Post-traumatic osteoarthritis: the role of stress induced chondrocyte damage. Biorheology 43:517-521.
11. Lane Smith R, Trindade MC, Ikenoue T, et al. 2000. Effects of shear stress on articular chondrocyte metabolism. Biorheology 37:95-107. (Pubitemid 30353539)
12. Smith RL, Donlon BS, Gupta MK, et al. 1995. Effects of fluid-induced shear on articular chondrocyte morphology and metabolism in vitro. J Orthop Res 13:824-831.
13. Mohtai M, Gupta MK, Donlon B, et al. 1996. Expression of interleukin-6 in osteoarthritic chondrocytes and effects of fluid-induced shear on this expression in normal human chondrocytes in vitro. J Orthop Res 14:67-73.
14. Lee MS, Trindade MCD, Ikenoue T, et al. 2003. Intermittent hydrostatic pressure inhibits shear stress-induced nitric oxide release in human osteoarthritic chondrocytes in vitro. J Rheumatol 30:326-328. (Pubitemid 36152599)
15. Lee MS, Trindade MCD, Ikenoue T, et al. 2003. Regulation of nitric oxide and bcl-2 expression by shear stress in human osteoarthritic chondrocytes in vitro. J Cell Biochem 90:80-86. (Pubitemid 37052311)
16. Huang J, Ballou LR, Hasty KA. 2007. Cyclic equibiaxial tensile strain induces both anabolic and catabolic responses in articular chondrocytes. Gene 404:101-109.
17. Brown TD, Bottlang M, Pedersen DR, et al. 1998. Loading paradigms - intentional and unintentional - for cell culture mechanostimulus. Am J Med Sci 316:162-168.
18. Owan I, Burr DB, Turner CH, et al. 1997. Mechanotransduction in bone: osteoblasts are more responsive to fluid forces than mechanical strain. Am J Physiol 273(Pt 1):C810-C815.
19. Brown TD. 2000. Techniques for mechanical stimulation of cells in vitro: a review. J Biomech 33:3-14.
20. Kuettner KE, Memoli VA, Pauli BU, et al. 1982. Synthesis of cartilage matrix by mammalian chondrocytes in vitro. II. Maintenance of collagen and proteoglycan phenotype. J Cell Biol 93:751-757.
21. Jones DG, Smith RL. 1990. Stimulation of adult chondrocyte metabolism by a thyroid-derived factor. J Orthop Res 8:227-233.
22. Xie Y, Sproule T, Li Y, et al. 2002. Nanoscale modifications of PET polymer surfaces via oxygen-plasma discharge yield minimal changes in attachment and growth of mammalian epithelial and mesenchymal cells in vitro. J Biomed Mater Res 61:234-245.
23. Green LC, Wagner DA, Glogowski J, et al. 1982. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem 126:131-138.
24. Blanco FJ, Ochs RL, Schwarz H, et al. 1995. Chondrocyte apoptosis induced by nitric oxide. Am J Pathol 146:75-85.
25. Liu J, Liu T, Zheng Y, et al. 2006. Early responses of osteoblast-like cells to different mechanical signals through various signaling pathways. Biochem Biophys Res Commun 348:1167-1173.
26. Zaman G, Pitsillides AA, Rawlinson SC, et al. 1999. Mechanical strain stimulates nitric oxide production by rapid activation of endothelial nitric oxide synthase in osteocytes. J Bone Miner Res 14:1123-1131.
27. Meazzini MC, Toma CD, Schaffer JL, et al. 1998. Osteoblast cytoskeletal modulation in response to mechanical strain in vitro. J Orthop Res 16:170-180.
28. Tanaka SM, Sun HB, Roeder RK, et al. 2005. Osteoblast responses one hour after load-induced fluid flow in a three-dimensional porous matrix. Calcif Tissue Int 76:261-271.
29. Choi JB, Youn I, Cao L, et al. 2007. Zonal changes in the three-dimensional morphology of the chondron under compression: the relationship among cellular, pericellular, and extracellular deformation in articular cartilage. J Biomech 40:2596-2603.
30. Butler DL, Goldstein SA, Guilak F. 2000. Functional tissue engineering: the role of biomechanics. J Biomech Eng 122:570-575.
31. Goreham-Voss CM, McKinley TO, Brown TD. 2007. A finite element exploration of cartilage stress near an articular incongruity during unstable motion. J Biomech 40:3438-3447.
32. Jessop HL, Rawlinson SCF, Pitsillides AA, et al. 2002. Mechanical strain and fluid movement both activate extracellular regulated kinase (ERK) in osteoblast-like cells but via different signaling pathways. Bone 31:186-194.
33. Wang JH, Thampatty BP. 2006. An introductory review of cell mechanobiology. Biomech Model Mechanobiol 5:1-16.
34. Carter DR, Wong M. 2003. Modelling cartilage mechanobiology. Philos Trans R Soc Lond B Biol Sci 358:1461-1471.
35. Smith RL, Carter DR, Schurman DJ. 2004. Pressure and shear differentially alter human articular chondrocyte metabolism: a review. Clin Orthop Relat Res 427(Suppl):S89-S95.
36. Waldman SD, Couto DC, Grynpas MD, et al. 2007. Multi-axial mechanical stimulation of tissue engineered cartilage: review. Eur Cell Mater 13:66-75.
37. Wong M, Siegrist M, Cao X. 1999. Cyclic compression of articular cartilage explants is associated with progressive consolidation and altered expression pattern of extracellular matrix proteins. Matrix Biol 18:391-399.
38. Katta J, Stapleton T, Ingham E, et al. 2008. The effect of glycosaminoglycan depletion on the friction and deformation of articular cartilage. Proc Inst Mech Eng H 222:1-11.
39. Lu XL, Mow VC. 2008. Biomechanics of articular cartilage and determination of material properties. Med Sci Sports Exerc 40:193-199.
40. Schmidt MB, Mow VC, Chun LE, et al. 1990. Effects of proteoglycan extraction on the tensile behavior of articular cartilage. J Orthop Res 8:353-363.
41. Zhu W, Mow VC, Koob TJ, et al. 1993. Viscoelastic shear properties of articular cartilage and the effects of glycosidase treatments. J Orthop Res 11:771-781. (Pubitemid 24040158)
42. Das P, Schurman DJ, Smith RL. 1997. Nitric oxide and G proteins mediate the response of bovine articular chondrocytes to fluid-induced shear. J Orthop Res 15:87-93.
43. Murrell GA, Jang D, Williams RJ. 1995. Nitric oxide activates metalloprotease enzymes in articular cartilage. Biochem Biophys Res Commun 206:15-21.
44. Squires GR, Okouneff S, Ionescu M, et al. 2003. The pathobiology of focal lesion development in aging human articular cartilage and molecular matrix changes characteristic of osteoarthritis. Arthritis Rheum 48:1261-1270.
45. Waldman SD, Spiteri CG, Grynpas MD, et al. 2003. Long-term intermittent shear deformation improves the quality of cartilaginous tissue formed in vitro. J Orthop Res 21:590-596.
46. Wall ME, Weinhold PS, Siu T, et al. 2007. Comparison of cellular strain with applied substrate strain in vitro. J Biomech. 40:173-181.
47. Wang Y, Chang J, Chen K, et al. 2007. Selective adapter recruitment and differential signaling networks by VEGF vs. shear stress. Proc Natl Acad Sci USA 104:8875-8879.
48. Chiquet M, Tunç-Civelek V, Sarasa-Renedo A. 2007. Gene regulation by mechanotransduction in fibroblasts. Appl Physiol Nutr Metab 32:967-973.