Quality traits of conventional and transgenic lettuce (Lactuca sativa L.) at harvesting by NMR metabolic profiling

Journal of Agricultural and Food Chemistry

Volumn 58, Issue 11, 2010, Pages 6928-6936

  Sobolev, Anatoly P ^a   Testone, Giulio ^a   Santoro, Flavio ^a   Nicolodi, Chiara ^a   Iannelli, Maria A ^a   Amato, Maria E ^b   Ianniello, Antonietta ^a   Brosio, Elvino ^c   Giannino, Donato ^a   Mannina, Luisa ^a,c  

^a CNR   (Italy)

^b UNIVERSITY OF CATANIA   (Italy)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Gene transcription profiling; Nitrogen status; NMR metabolic profiling; Transgenic lettuce (Lactuca sativa L.)

Indexed keywords

AMINO ACID; VEGETABLE PROTEIN;

ARTICLE; CHEMISTRY; COMPARATIVE STUDY; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; LETTUCE; METABOLISM; METABOLOME; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PLANT LEAF; TRANSGENIC PLANT;

AMINO ACIDS; GENE EXPRESSION REGULATION, PLANT; LETTUCE; MAGNETIC RESONANCE SPECTROSCOPY; METABOLOME; PLANT LEAVES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED;

ESCHERICHIA COLI; LACTUCA; LACTUCA SATIVA;

EID: 77953159220     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf904439y     Document Type: Article

References (42)

1. Fernie, A. R.; Schauer, N. Metabolomics-assisted breeding: a viable option for crop improvement? Trends Genet. 2009, 25, 39-48
2. Dixon, R. A.; Gang, D. R.; Charlton, A. J.; Fiehn, O.; Kuiper, H. A.; Reynolds, T. L.; Tjeerdema, R. S.; Jeffery, E. H.; German, J. B.; Ridley, W. P.; Seiber, J. N. Applications of metabolomics in agriculture J. Agric. Food Chem. 2006, 54, 8984-8994
3. Beiquan, M. Lettuce. In Vegetables I. Asteraceae, Brassicaceae, Chenopodicaceae, and Cucurbitaceae; Nuez, F.; Prohens, J., Ed.; Springer: New York, 2008; Vol. 1, pp 75 - 116.
4. Santamaria, P. Nitrate in vegetables: toxicity, content, intake and EC regulation J. Sci. Food Agric. 2006, 86, 10-17
5. Santamaria, P.; Elia, A.; Gonnella, M. Changes in nitrate accumulation and growth of endive plants during the light period as affected by nitrogen level and form J. Plant Nutr. 1997, 20, 1255-1266
6. Broadley, M. R.; Seginer, I.; Burns, A.; Escobar-Gutierrez, A. J.; Burns, I. G.; White, P. J. The nitrogen and nitrate economy of butterhead lettuce (Lactuca sativa var capitata L) J. Exp. Bot. 2003, 54, 2081-2090
7. Reinink, K. Genetics of nitrate content of lettuce, 1: Analysis of generation means Euphytica 1991, 54, 83-92
8. Curtis, I.; JB, P.; AMM, d. L.; M, C.; MR, D. Expression of a chimeric nitrate reductase gene in transgenic lettuce reduces nitrate in leaves Plant Cell Rep. 1999, 18, 889-896
9. Stitt, M.; Muller, C.; Matt, P.; Gibon, Y.; Carillo, P.; Morcuende, R.; Scheible, W. R.; Krapp, A. Steps towards an integrated view of nitrogen metabolism J. Exp. Bot. 2002, 53, 959-970
10. Lea, P. J.; Sodek, L.; Parry, M. A. J.; Shewry, P. R.; Halford, N. G. Asparagine in plants Ann. Appl. Biol. 2007, 150, 1-26
11. Richards, N. G.; Schuster, S. M. Mechanistic issues in asparagine synthetase catalysis Adv. Enzymol. Relat. Areas Mol. Biol. 1998, 72, 145-198
12. Giannino, D.; Nicolodi, C.; Testone, G.; Frugis, G.; Pace, E.; Santamaria, P.; Guardasole, M.; Mariotti, D. The overexpression of asparagine synthetase A from E. coli affects the nitrogen status in leaves of lettuce (Lactuca sativa L.) and enhances vegetative growth Euphytica 2008, 162, 11-22
13. Sobolev, A. P.; Segre, A. L.; Giannino, D.; Mariotti, D.; Nicolodi, C.; Brosio, E.; Amato, M. E. Strong increase of foliar inulin occurs in transgenic lettuce plants (Lactuca sativa L.) overexpressing the asparagine synthetase A gene from Escherichia coli J. Agric. Food Chem. 2007, 55, 10827-10831
14. Griffiths, G.; Leverentz, M.; Silkowski, H.; Gill, N.; Sanchez-Serrano, J. J. Lipid hydroperoxides in plants Biochem. Soc. Trans. 2000, 28, 837-839
15. Bligh, E. G.; Dyer, W. J. A rapid method of total lipid extraction and purification Can. J. Biochem. Physiol. 1959, 37, 911-917
16. Sobolev, A. P.; Brosio, E.; Gianferri, R.; Segre, A. L. Metabolic profile of lettuce leaves by high-field NMR spectra Magn. Reson. Chem. 2005, 43, 625-638
17. Martens, H.; Martens, M. MultiVariate Analysis of Quality: An Introduction; Wiley: Chichester, U.K., 2001.
18. Livak, K. J.; Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method Methods 2001, 25, 402-408
19. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 1976, 72, 248-254
20. Puebla, A. F.; Battaglia, M. E.; Salerno, G. L.; Pontis, H. G. Sucrose-sucrose fructosyl transferase activity: A direct and rapid colorimetric procedure for the assay of plant extracts Plant Physiol. Biochem. 1999, 37, 699-702
21. Mattoo, A. K.; Sobolev, A. P.; Neelam, A.; Goyal, R. K.; Handa, A. K.; Segre, A. L. Nuclear magnetic resonance spectroscopy-based metabolite profiling of transgenic tomato fruit engineered to accumulate spermidine and spermine reveals enhanced anabolic and nitrogen-carbon interactions Plant Physiol. 2006, 142, 1759-1770
22. Eckhardt, U.; Grimm, B.; Hortensteiner, S. Recent advances in chlorophyll biosynthesis and breakdown in higher plants Plant Mol. Biol. 2004, 56, 1-14
23. Brears, T.; Liu, C.; Knight, T. J.; Coruzzi, G. M. Ectopic overexpression of asparagine synthetase in transgenic tobacco Plant Physiol 1993, 103, 1285-1290
24. Lam, H. M.; Wong, P.; Chan, H. K.; Yam, K. M.; Chen, L.; Chow, C. M.; Coruzzi, G. M. Overexpression of the ASN1 gene enhances nitrogen status in seeds of Arabidopsis Plant Physiol. 2003, 132, 926-935
25. Seiffert, B.; Zhou, Z.; Wallbraun, M.; Lohaus, G.; Mollers, C. Expression of a bacterial asparagine synthetase gene in oilseed rape (Brassica napus) and its effect on traits related to nitrogen efficiency Physiol. Plant 2004, 121, 656-665
26. Dudits, D.; Paulovics, K.; Kalmann, K.; Gyorgyey, J.; Nagy, F.; Bako, L.; Horvarth, G.; Eckes, P.; Donn, G. Transgenic Plants Expressing a Prokaryotic Ammonium Dependent Asparagine Synthetase. U.S. Patent 5545819, 1991.
27. Bellucci, M.; Ederli, L.; De Marchis, F.; Pasqualini, S.; Arcioni, S. Transformation of Lotus corniculatus plants with Escherichia coli asparagine synthetase A: effect on nitrogen assimilation and plant development Plant Cell, Tissue Organ Cult. 2004, 78, 139-150
28. Miller, A. J.; Fan, X.; Shen, Q.; Smith, S. J. Amino acids and nitrate as signals for the regulation of nitrogen acquisition J. Exp. Bot. 2008, 59, 111-119
29. Imsande, J.; Touraine, B. N demand and the regulation of nitrate uptake Plant Physiol. 1994, 105, 3-7
30. Lam, H. M.; Hsieh, M. H.; Coruzzi, G. Reciprocal regulation of distinct asparagine synthetase genes by light and metabolites in Arabidopsis thaliana Plant J. 1998, 16, 345-353
31. Oliveira, I. C.; Brenner, E.; Chiu, J.; Hsieh, M. H.; Kouranov, A.; Lam, H. M.; Shin, M. J.; Coruzzi, G. Metabolite and light regulation of metabolism in plants: lessons from the study of a single biochemical pathway Braz. J. Med. Biol. Res. 2001, 34, 567-575
32. Bernard, S. M.; Habash, D. Z. The importance of cytosolic glutamine synthetase in nitrogen assimilation and recycling New Phytol. 2009, 182, 608-620
33. Glass, A. D.; Britto, D. T.; Kaiser, B. N.; Kinghorn, J. R.; Kronzucker, H. J.; Kumar, A.; Okamoto, M.; Rawat, S.; Siddiqi, M. Y.; Unkles, S. E.; Vidmar, J. J. The regulation of nitrate and ammonium transport systems in plants J. Exp. Bot. 2002, 53, 855-864
34. Meyer, C.; Stitt, M. Nitrate Reduction and Signalling. In Plant Nitrogen; Lea, P. J.; Morot-Gaudry, J. F., Eds.; Springer-Verlag: Berlin, 2001; pp 39 - 59.
35. Tsay, Y. F.; Chiu, C. C.; Tsai, C. B.; Ho, C. H.; Hsu, P. K. Nitrate transporters and peptide transporters FEBS Lett. 2007, 581, 2290-2300
36. Gojon, A.; Dapoigny, L.; Lejay, L.; Tillard, P.; Rufty, T. W. Effects of genetic modifications of nitrate reductase expression on 15NO3 uptake and reduction in Nicotiana plants Plant Cell Environ. 1998, 21, 43-53
37. Lea, U. S.; Leydecker, M. T.; Quillere, I.; Meyer, C.; Lillo, C. Posttranslational regulation of nitrate reductase strongly affects the levels of free amino acids and nitrate, whereas transcriptional regulation has only minor influence Plant Physiol. 2006, 140, 1085-94
38. Ritsema, T.; Smeekens, S. Fructans: beneficial for plants and humans Curr. Opin. Plant Biol. 2003, 6, 223-30
39. Chalmers, J.; Lidgett, A.; Cummings, N.; Cao, Y.; Forster, J.; Spangenberg, G. Molecular genetics of fructan metabolism in perennial ryegrass Plant Biotechnol. J. 2005, 3, 459-474
40. Yin, Z.; Plader, W.; Malepszy, S. Transgene inheritance in plants J. Appl. Genet. 2004, 45, 127-144
41. Van den Ende, W.; Michiels, A.; Van Wonterghem, D.; Vergauwen, R.; Van Laere, A. Cloning, developmental, and tissue-specific expression of sucrose:sucrose 1-fructosyl transferase from Taraxacum officinale. Fructan localization in roots Plant Physiol. 2000, 123, 71-80
42. Vijn, I.; van Dijken, A.; Luscher, M.; Bos, A.; Smeets, E.; Weisbeek, P.; Wiemken, A.; Smeekens, S. Cloning of sucrose:sucrose 1-fructosyltransferase from onion and synthesis of structurally defined fructan molecules from sucrose Plant Physiol. 1998, 117, 1507-1513