Characterization of methylmalonyl-CoA mutase involved in the propionate photoassimilation of Euglena gracilis Z

Archives of Microbiology

Volumn 192, Issue 6, 2010, Pages 437-446

  Miyamoto, Emi ^b   Tanioka, Yuri ^c,f   Nishizawa Yokoi, Ayako ^d   Yabuta, Yukinori ^a   Ohnishi, Kouhei ^e   Misono, Haruo ^e   Shigeoka, Shigeru ^d   Nakano, Yoshihisa ^c   Watanabe, Fumio ^a  

^a TOTTORI UNIVERSITY   (Japan)

^b NAGASAKI INTERNATIONAL UNIVERSITY   (Japan)

^c Osaka Prefecture University   (Japan)

^d KINKI UNIVERSITY   (Japan)

^e KOCHI UNIVERSITY   (Japan)

^f TOKYO UNIVERSITY OF AGRICULTURE   (Japan)

Author keywords

Cobalamin; Euglena gracilis Z; Methylmalonyl CoA mutase; Photoassimilation; Propionate metabolism

Indexed keywords

AMINO ACID; APOENZYME; COBAMAMIDE; COMPLEMENTARY DNA; HOLOENZYME; METHYLMALONYL COENZYME A MUTASE; PROPIONIC ACID; PROTEIN SUBUNIT;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; EUGLENA GRACILIS; GEL FILTRATION; INCUBATION TIME; MITOCHONDRION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHOTOSYNTHESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN TARGETING; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; AMINO ACIDS; APOENZYMES; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; COBAMIDES; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EUGLENA GRACILIS; HOLOENZYMES; KINETICS; METHYLMALONYL-COA MUTASE; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PROPIONATES; PROTEIN SUBUNITS; PROTOZOAN PROTEINS; TEMPERATURE;

EUGLENA; EUGLENA GRACILIS; MASTIGOPHORA (FLAGELLATES);

EID: 77953028359     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00203-010-0572-x     Document Type: Article

References (35)

1. Ackrell BC, Kearney EB, Singer TP (1978) Mammalian succinate dehydrogenase. Meth Enzymol 53:466-483.
2. Amero SA, James TC, Elgin SCR (1988) Production of antibodies using proteins in gel bands. In: Walker JM (ed) Methods in molecular biology, vol 3. Humana Press, New Jersey, pp 355-362.
3. Bairoch A, Bucher P, Hofman K (1997) The PROSITE database, its status in 1997. Nucleic Acids Res 25:217-221.
4. Charles TC, Aneja P (1999) Methylmalonyl-CoA mutase encoding gene of Sinorhizobium meliloti. Gene 226:121-127.
5. Cramer MC, Myers J (1952) Growth and photosynthetic characterisitics of Euglena gracilis. Arch Microbiol 17:384-402.
6. Dobson CM, Wai T, Leclerc D, Wilson A, Wu X, Dore C, Hudson T, Rosenblatt DS, Gravel RA (2002) Identification of the gene responsible for the cblA complementation group of vitamin B12- responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements. Proc Natl Acad Sci USA 99:15554-15559.
7. 12: A 3.0 Å X-ray structure of B12- binding domains of methionine synthase. Science 266:1669-1674.
8. 12-dependent enzymes. Curr Opin Struct Biol 4:919-929.
9. Fenton WA, Hack AM, Willard HF, Gertler A, Rosenberg LE (1982) PuriWcation and properties of methylmalonyl coenzyme a mutase from human liver. Arch Biochem Biophys 214:815-826.
10. Francalanci F, Davis NK, Fuller JQ, Murfitt D, Leadlay PF (1986) The subunit structure of methylmalonyl-CoA mutase from Propioni-bacterium shermanii. Biochem J 236:489-494.
11. Frantz C, Ebel C, Paulus F, Imbaut P (2000) Characterization of transsplicing in Euglenoids. Curr Genet 37:349-355.
12. Gaire D, Sponne I, Droesch S, Charlier A, Nicolas JP, Lambert D (1999) Comparison of two methods for the measurement of rat liver methylmalonyl- coenzyme a mutase activity: HPLC and radioisotopic assays. J Nutr Biochem 10:56-62.
13. Han YS, Bratt JM, Hogenkamp HPC (1984) Purification and characterization of methylmalonyl-CoA mutase from Ascaris lumbricodes. Comp Biochem Physiol 78B:41-45.
14. Isegawa Y, Nakano Y, Kitaoka S (1984) Conversion and distribution of cobalamin in Euglena gracilis Z, with special reference to its location and probable function within chloroplasts. Plant Physiol 76:814-818.
15. Ishikawa T, Tajima N, Nishikawa H, Gao Y, Rapolu M, Shibata H, Sawa Y, Shigeoka S (2010) Euglena gracilis ascorbate peroxidase forms an intramolecular dimeric structure: its unique molecular characterization. Biochem J 426:125-134.
16. Jansen R, Kalousek F, Fenton WA, Rosenberg LE, Ledley FD (1989) Cloning of full-length methylmalonyl-CoA mutase from a cDNA library using the polymerase chain reaction. Genomics 4:198-205.
17. Kellermeyer RW, Allen SHG, Stjernhorm R, Wood HG (1964) Methylmalonyl Isomerase. J Biol Chem 239:2562-2569.
18. Kitaoka S, Nakano Y, Miyatake K, Yokota A (1989) Enzymes and their functional location. In: Buetow DE (ed) The biology of euglena, vol 4. Academic Press, San Diego, pp 1-135.
19. Korotkova N, Lidstrom ME (2004) MeaB is a component of the methylmalonyl-CoA mutase complex required for protection of the enzyme from inactivation. J Biol Chem 279:13652-13658.
20. 12 cofactors: the proteins make a difference. Structure 4:505-512.
21. Marsh ENG, Holloway DE (1992) Cloning and sequencing of glutamate mutase component S from Clostridium tetanomorphum Homologies with other cobalamin-dependent enzymes. FEBS Lett 310:167-170.
22. Miyamoto E, Watanabe F, Yamaji R, Inui I, Sato K, Nakano Y (2002) PuriWcation and characterization of methylmalonyl-CoA mutase from a ethanol-utilizing bacterium, Methylobacterium extorquens NR-1. J Nutr Sci Vitaminol 48:242-246.
23. Miyamoto E, Watanabe F, Charles TC, Yamaji R, Inui H, Nakano Y PuriWcation and characterization of homodimeric methylmalonyl-CoA mutase from Sinorhizobium meliloti. Arch Mirobiol 180:151-154.
24. Miyamoto E, Watanabe F, Yamaguchi Y, Takenaka H, Nakano Y PuriWcation and characterization of methylmalonyl-CoA mutase from a photosynthetic coccolithophorid alga, Pleurochrysis carterae. Comp Biochem Physiol B 138:163-167.
25. Miyamoto E, Tanioka Y, Yukino Y, Hayashi M, Watanabe F, Nakano Y (2007) Occurrence of 5'-deoxyadenosylcobalamin and its physiological function as the coenzyme of metnylmalonyl-CoA mutase in a marine eukaryotic microorganism, Schizochytrium limacinum SR21. J Nutr Sci Vitaminol 53:471-475.
26. Padovani D, Banerjee R (2006) Assembly and protection of the radical enzyme, methylmalonyl-CoA mutase, by its chaperone. Biochemistry 45:9300-9306.
27. Tessier LH, Keller M, Chan RL, Fournier R, Weil JH, Imbault P (1991) Short leader sequences may be transferred from small RNAs to premature mRNA by trans-splicing in Euglena. EMBO J 10:2621-2625.
28. Tokunaga M, Nakano T, Kitaoka S (1976) Separation and properties of the NAD-linked and NADP-linked isozymes of succinic semialdehyde dehydrogenase in Euglena gracilis Z. Biochim Biophys Acta 429:55-62.
29. Watanabe F, Nakano Y, Kitaoka S (1987) Isolation and some properties of soluble and membrane-bound cobalamin binding proteins of Euglena mitochondira. Arch Microbiol 149:30-35.
30. Watanabe F, Nakano Y, Stupperich E (1992) Different corrinoid specificities for cell growth and the cobalamin uptake system in Euglena gracilis Z. J Gen Microbiol 138:1807-1813.
31. Watanabe F, Tamura Y, Stupperich E, Nakano Y (1993) Uptake of cobalamin by Euglena mitochondria. J Biochem 114:793-799.
32. Watanabe F, Abe K, Tamura Y, Nakano Y (1996) Adenosylcobalamin-dependent methylmalonyl-CoA mutase isozymes in the photosynthetic protozoon Euglena gracilis Z. Microbiology 142:2631-2634.
33. Wilkemeyer MF, Crane AM, Ledley FD (1990) Primary structure and activity of mouse methylmalonyl-CoA mutase. Biochem J 271:449-455.
34. Yokota A, Hosotani K, Kitaoka S (1982) Mechanism of metabolic regulation in photoassimilation of propionate in Euglena gracilis Z. Arch Biochem Biophys 249:530-537.
35. Zeng S, Gong Z (2002) Expressed sequence tag analysis of expression profiles of zebrafish testis and ovary. Gene 294:45-53.