ACBP knockdown leads to down-regulation of genes encoding rate-limiting enzymes in cholesterol and fatty acid metabolism

Cellular Physiology and Biochemistry

Volumn 25, Issue 6, 2010, Pages 675-686

  Vock, Christina ^a   Biedasek, Katrin ^b   Boomgaarden, Inka ^a   Heins, Anja ^a   Nitz, Inke ^a   Döring, Frank ^a  

^a UNIVERSITY OF KIEL   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

ACBP; Cholesterol metabolism; Fatty acids; HepG2 cells; RNAi

Indexed keywords

CHOLESTEROL; DIAZEPAM BINDING INHIBITOR; ENZYME; FATTY ACID; FATTY ACID SYNTHASE; GLYCEROL 3 PHOSPHATE ACYLTRANSFERASE; GLYCEROLIPID; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; HYDROXYMETHYLGLUTARYL COENZYME A SYNTHASE; MONOUNSATURATED FATTY ACID; SMALL INTERFERING RNA; UNSATURATED FATTY ACID;

ARTICLE; CELL STRAIN HEPG2; CHOLESTEROL METABOLISM; CONTROLLED STUDY; DOWN REGULATION; FATTY ACID METABOLISM; GENE CONTROL; GENE EXPRESSION; GENE TARGETING; HUMAN; HUMAN CELL; LIPOGENESIS; METABOLITE; PRIORITY JOURNAL; PROTEIN FUNCTION; UPREGULATION; APOPTOSIS; GENE; GENETICS; HEPG2 CELL LINE; METABOLISM;

MAMMALIA;

APOPTOSIS; CHOLESTEROL; DIAZEPAM BINDING INHIBITOR; DOWN-REGULATION; FATTY ACIDS; GENES; HEP G2 CELLS; HUMANS; RNA, SMALL INTERFERING;

EID: 77952991123     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000315087     Document Type: Article

References (59)

1. Mandrup S, Hummel R, Ravn S, Jensen G, Andreasen PH, Gregersen N, Knudsen J, Kristiansen K: Acyl-CoA-binding protein/diazepam-binding inhibitor gene and pseudogenes. A typical housekeeping gene family. J Mol Biol 1992;228:1011-1022.
2. Gray PW, Glaister D, Seeburg PH, Guidotti A, Costa E: Cloning and expression of cDNA for human diazepam binding inhibitor, a natural ligand of an allosteric regulatory site of the gamma-aminobutyric acid type A receptor. Proc Nat Acad Sci USA 1986;83:7547-7551.
3. Guidotti A, Forchetti CM, Corda MG, Konkel D, Bennett CD, Costa E: Isolation, characterization, and purification to homogeneity of an endogenous polypeptide with agonistic action on benzodiazepine receptors. Proc Natl Acad Sci USA 1983;80:3531-3535.
4. Borboni P, Condorelli L, De Stefanis P, Sesti G, Lauro R: Modulation of insulin secretion by diazepam binding inhibitor and its processing products. Neuro-pharmacology 1991;30:1399-1403.
5. Chen ZW, Agerberth B, Gell K, Andersson M, Mutt V, Ostenson CG, Efendic S, Barrossoderling J, Persson B, Jornvall H: Isolation ad characterization of porcine diazepam-binding inhibitor, apolypeptide not only of cerebral occurrence but also common in intestinal tissues and with effects on regulation of insulin resistance. Eur J Biochem 1988;174:239-245.
6. Herzig KH, Schon I, Tatemoto K, Ohe Y, Li Y, Folsch UR, Owyang C: Diazepam binding inhibitor is a potent cholecystokinin releasing peptide in the intestine (vol 93, pg 7927, 1996). Proc Nat Acad Sci USA 1996;93:14214-14214
7. Besman MJ, Yanagibashi K, Lee TD, Kawamura M, Hall PF, Shively JE: Identification of des-(Gly-Ile)-endo-zepine as an effector of corticotropin-dependent adrenal steroidogenesis: stimulation of cholesterol delivery is mediated by the peripheral benzodia-zepine receptor. Proc Natl Acad Sci USA 1989;86:4897-4901.
8. Boujrad N, Hudson JR, Jr., Papadopoulos V: Inhibition of hormone-stimulated steroidogenesis in cultured Leydig tumor cells by a cholesterol-linked phosphoro-thioate oligodeoxynucleotide antisense to diazepam-binding inhibitor. Proc Natl Acad Sci USA 1993;90:5728-5731.
9. Papadopoulos V, Brown AS: Role of the peripheral-type benzodiazepine receptor and the polypeptide diazepam binding inhibitor in steroidogenesis. J Steroid Biochem Mol Biol 1995;53:103-110.
10. Garnier M, Boujrad N, Oke BO, Brown AS, Riond J, Ferrara P, Shoyab M, Suarez-Quian CA, Papadopoulos V: Diazepam binding inhibitor is a paracrine/autocrine regulator of Leydig cell proliferation and steroidogenesis: action via peripheral-type benzodiazepine receptor and independent mechanisms. Endocrinology 1993;132:444-458.
11. Melloni E, Averna M, Salamino F, Sparatore B, Minafra R, Pontremoli S: Acyl-CoA-binding protein is a potent m-calpain activator. J Biol Chem 2000;275:82-86.
12. Shulga N, Pastorino JG: Acyl coenzyme A-binding protein augments bid-induced mitochondrial damage and cell death by activating mu-calpain. J Biol Chem 2006;281:30824-30833.
13. Faergeman NJ, Sigurskjold BW, Kragelund BB, Andersen KV, Knudsen J: Thermodynamics of ligand binding to acyl-coenzyme A binding protein studied by titration calorimetry. Biochemistry 1996;35:14118-14126.
14. Wadum MCT, Villadsen JK, Feddersen S, Moller RS, Neergaard TBF, Kragelund BB, Hojrup P, Faergeman NJ, Knudsen J: Fluorescently labelled bovine acyl-CoA-binding protein acting as an acyl-CoA sensor: Interaction with CoA and acyl-CoA esters and its use in measuring free acyl-CoA esters and non-esterified fatty acids. Biochem J 2002;365:165-172.
15. Rosendal J, Ertbjerg P, Knudsen J: Characterization of ligand binding to acyl-CoA-binding protein. Biochem J 1993;290:321-326.
16. Knudsen J, Faergeman NJ, Skott H, Hummel R, Borsting C, Rose TM, Andersen JS, Hojrup P, Roepstorff P, Kristiansen K: Yeast acyl-CoA-binding protein: acyl-CoA-binding affinity and effect on intracellular acyl-CoA pool size. Biochem J 1994;302:479-485.
17. Mandrup S, Jepsen R, Skott H, Rosendal J, Hojrup P, Kristiansen K, Knudsen J: Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast. Biochem J 1993;290:369-374.
18. Rasmussen JT, Faergeman NJ, Kristiansen K, Knudsen J: Acyl-CoA binding protein (ACBP) can mediate intermembrane acyl-CoA transport and donate acyl-CoA for beta-oxidation and glycerolipid synthesis. Biochem J 1994;299:165-170.
19. Rasmussen JT, Rosendal J, Knudsen J: Interaction of acyl-CoA-binding protein (ACBP) on processes for which acyl-CoA is a substrate, product or inhibitor. Biochem J 1993;292:907-913.
20. Cohen Simonsen A, Bernchou Jensen U, Faergeman NJ, Knudsen J, Mouritsen OG: Acyl-coenzyme A organizes laterally in membranes and is recognized specifically by acyl-coenzyme A binding protein. FEBS Lett 2003;552:253-258.
21. Gaigg B, Neergaard TBF, Schneiter R, Hansen JK, Faergeman NJ, Jensen NA, Andersen JR, Friis J, Sandhoff R, Schroder HD, Knudsen J: Depletion of acyl-coenzyme A-binding protein affects sphingolipid synthesis and causes vesicle accumulation and membrane defects in Saccharomyces cerevisiae. Mol Biol Cell 2001;12:1147-1160.
22. Lee L, DeBono CA, Campagna DR, Young DC, Moody DB, Fleming MD: Loss of the acyl-CoA binding protein (Acbp) results in fatty acid metabolism abnormalities in mouse hair and skin. J Invest Dermatol 2007;127:16-23.
23. Hansen JS, Faergeman NJ, Kagelund BB, Knudsen J: Acyl-CoA binding protein (ACBP) localizes to ER and Golgi in a ligand dependent manner in mammalian cells. Biochem J 2008;410 (3):463-472.
24. Petrescu AD, Payne HR, Boedecker A, Chao H, Hertz R, Bar-Tana J, Schroeder F, Kier AB: Physical and functional interaction of acyl-CoA-binding protein with hepatocyte nuclear factor-4 alpha. J Biol Chem 2003;278:51813- 51824.
25. Helledie T, Antonius M, Sorensen RV, Hertzel AV, Bernlohr DA, Kolvraa S, Kristiansen K, Mandrup S: Lipid-binding proteins modulate ligand-dependent trans-activation by peroxisome proliferator-activated receptors and localize to the nucleus as well as the cytoplasm. J Lipid Res 2000;41:1740-1751.
26. Feddersen S, Neergaard TB, Knudsen J, Faergeman NJ: Transcriptional regulation of phospholipid biosynthesis is linked to fatty acid metabolism by an acyl-CoA binding protein dependent mechanism in Saccharomyces cerevisiae. Biochem J 2007;407:219-230.
27. Reynolds A, Leake D, Boese Q, Scaringe S, Marshall WS, Khvorova A: Rational siRNA design for RNA interference. Nature Biotechnology 2004;22:326-330.
28. Zuker M: Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 2003;31:3406-3415.
29. Faergeman NJ, Knudsen J: Acyl-CoA binding protein is an essential protein in mammalian cell lines. Biochem J 2002;368:679-682.
30. Vock C, Doring F, Nitz I: Transcriptional regulation of HMG-CoA synthase and HMG-CoA reductase genes by human ACBP. Cell Physiol Biochem 2008;22:515-524.
31. Liu WM, Mei R, Di X, Ryder TB, Hubbell E, Dee S, Webster TA, Harrington CA, Ho MH, Baid J, Smeekens SP: Analysis of high density expression microarrays with signed-rank call algorithms. Bioinformatics 2002;18:1593-1599.
32. Dennis G, Jr., Sherman BT, Hosack DA, Yang J, Gao W, Lane HC, Lempicki RA: DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol 2003;4:P3.
33. Scherf M, Epple A, Werner T: The next generation of literature analysis: integration of genomic analysis into text mining. Brief Bioinform 2005;6:287-297.
34. Cartharius K, Frech K, Grote K, Klocke B, Haltmeier M, Klingenhoff A, Frisch M, Bayerlein M, Werner T: MatInspector and beyond: promoter analysis based on transcription factor binding sites. Bioinformatics 2005;21:2933-2942.
35. Klingenhoff A, Frech K, Quandt K, Werner T: Functional promoter modules can be detected by formal models independent of overall nucleotide sequence similarity. Bioinformatics 1999;15:180-186.
36. Solstad T, Fismen L, Garberg H, Fladmark KE: Identification of a novel phosphorylation site of acyl-CoA binding protein (ACBP) in nodularin-induced apoptotic hepatocytes. Exp Cell Res 2008;314:2141-2149.
37. Helledie T, Jorgensen C, Antonius M, Krogsdam AM, Kratchmarova I, Kristiansen K, Mandrup S: Role of adipocyte lipid-binding protein (ALBP) and acyl-coA binding protein (ACBP) in PPAR-mediated transactivation. Mol Cell Biochem 2002;239:157-164.
38. Elholm M, Garras A, Neve S, Tornehave D, Lund TB, Skorve J, Flatmark T, Kristiansen K, Berge RK: Long-chain acyl-CoA esters and acyl-CoA binding protein are present in the nucleus of rat liver cells. J Lipid Res 2000;41:538-545.
39. Weisschuh N, Alavi MV, Bonin M, Wissinger B: Identification of genes that are linked with optineurin expression using a combined RNAi-microarray approach. Exp Eye Res 2007;85:450-461.
40. Shoda J, Inada Y, Tsuji A, Kusama H, Ueda T, Ikegami T, Suzuki H, Sugiyama Y, Cohen DE, Tanaka N: Bezafibrate stimulates canalicular localization of NBD-labeled PC in HepG2 cells by PPARalpha-mediated redistribution of ABCB4. J Lipid Res 2004;45:1813-1825.
41. Evans JR, Kelly DL, Morris KJ, Arvide EM, Harris A: RNA interference-mediated inhibition of hepatocyte nuclear factor 1alpha identifies target genes. Biochim Biophys Acta 2008;1779:341-346.
42. Huang H, Atshaves BP, Frolov A, Kier AB, Schroeder F: Acyl-coenzyme A binding protein expression alters liver fatty acyl-coenzyme A metabolism. Biochemistry 2005;44:10282-10297. (Pubitemid 41076823)
43. Oikari S, Ahtialansaari T, Heinonen MV, Mauriala T, Auriola S, Kiehne K, Folsch UR, Janne J, Alhonen L, Herzig KH: Downregulation of PPARs and SREBP by acyl-CoA-binding protein over-expression in transgenic rats. Pflugers Arch 2008;456:369-377
44. Milne KG, Guther ML, Ferguson MA: Acyl-CoA binding protein is essential in bloodstream form Trypanosoma brucei. Mol Biochem Parasitol 2001;112:301-304.
45. Schjerling CK, Hummel R, Hansen JR, Borsting C, Mikkelsen JM, Kristiansen K, Knudsen J: Disruption of the gene encoding the acyl-CoA-binding protein (ACB1) perturbs acyl-CoA metabolism in Saccharomyces cerevisiae. J Biol Chem 1996;271:22514-22521.
46. Ohgami RS, Campagna DR, Antiochos B, Wood EB, Sharp JJ, Barker JE, Fleming MD: nm1054: a spontaneous, recessive, hypochromic, microcytic anemia mutation in the mouse. Blood 2005;106:3625-3631.
47. Mandrup S, Sorensen RV, Helledie T, Nohr J, Baldursson T, Gram C, Knudsen J, Kristiansen K: Inhibition of 3T3-L1 adipocyte differentiation by expression of acyl-CoA-binding protein antisense RNA. J Biol Chem 1998;273:23897-23903.
48. Solstad T, Fismen L, Garberg H, Fladmark KE: Identification of a novel phosphorylation site of acyl-CoA binding protein (ACBP) in nodularin-induced apoptotic hepatocytes. Exp Cell Res 2008;314:2141-2149.
49. Faergeman NJ, Feddersen S, Christiansen JK, Larsen MK, Schneiter R, Ungermann C, Mutenda K, Roepstorff P, Knudsen J: Acyl-CoA-binding protein, Acb1p, is required for normal vacuole function and ceramide synthesis in Saccharomyces cerevisiae. Biochem J 2004;380:907-918.
50. Moon YA, Shah NA, Mohapatra S, Warrington JA, Horton JD: Identification of a mammalian long chain fatty acyl elongase regulated by sterol regulatory element-binding proteins. J Biol Chem 2001;276:45358-45366.
51. Matsuzaka T, Shimano H, Yahagi N, Kato T, Atsumi A, Yamamoto T, Inoue N, Ishikawa M, Okada S, Ishigaki N, Iwasaki H, Iwasaki Y, Karasawa T, Kumadaki S, Matsui T, Sekiya M, Ohashi K, Hasty AH, Nakagawa Y, Takahashi A, Suzuki H, Yatoh S, Sone H, Toyoshima H, Osuga J, Yamada N: Crucial role of a long-chain fatty acid elongase, Elovl6, in obesity-induced insulin resistance. Nat Med 2007;13:1193-1202.
52. Das AK, Uhler MD, Hajra AK: Molecular cloning and expression of mammalian peroxisomal trans-2-enoyl-coenzyme A reductase cDNAs. J Biol Chem 2000;275:24333-24340.
53. Glatz JF, van der Vusse GJ: Cellular fatty acid-binding proteins: their function and physiological significance. Prog Lipid Res 1996;35:243-282.
54. Giot L, Bader JS, Brouwer C, Chaudhuri A, Kuang B, Li Y, Hao YL, Ooi CE, Godwin B, Vitols E, Vijayadamodar G, Pochart P, Machineni H, Welsh M, Kong Y, Zerhusen B, Malcolm R, Varrone Z, Collis A, Minto M, Burgess S, McDaniel L, Stimpson E, Spriggs F, Williams J, Neurath K, Ioime N, Agee M, Voss E, Furtak K, Renzulli R, Aanensen N, Carrolla S, Bickelhaupt E, Lazovatsky Y, DaSilva A, Zhong J, Stanyon CA, Finley RL, Jr., White KP, Braverman M, Jarvie T, Gold S, Leach M, Knight J, Shimkets RA, McKenna MP, Chant J, Rothberg JM: A protein interaction map of Drosophila melanogaster. Science 2003;302:1727-1736.
55. Dolt KS, Karar J, Mishra MK, Salim J, Kumar R, Grover SK, Qadar Pasha MA: Transcriptional downregulation of sterol metabolism genes in murine liver exposed to acute hypobaric hypoxia. Biochem Biophys Res Commun 2007;354:148-153.
56. Kato M, Wickner W: Ergosterol is required for the Sec18/ATP-dependent priming step of homotypic vacuole fusion. Embo J 2001;20:4035-4040.
57. Shimano H, Horton JD, Hammer RE, Shimomura I, Brown MS, Goldstein JL: Overproduction of cholesterol and fatty acids causes massive liver enlargement in transgenic mice expressing truncated SREBP-1a. J Clin Invest 1996;98:1575-1584.
58. Eberle D, Hegarty B, Bossard P, Ferre P, Foufelle F: SREBP transcription factors: master regulators of lipid homeostasis. Biochimie 2004;86:839-848.
59. Bennett MK, Lopez JM, Sanchez HB, Osborne TF: Sterol regulation of fatty acid synthase promoter. Coordinate feedback regulation of two major lipid pathways. J Biol Chem 1995;270:25578-25583.