Green-fluorescent protein from the bioluminescent jellyfish Clytia gregaria: CDNA cloning, expression, and characterization of novel recombinant protein

Photochemical and Photobiological Sciences

Volumn 9, Issue 6, 2010, Pages 757-765

  Markova, Svetlana V ^a,b   Burakova, Ludmila P ^a   Frank, Ludmila A ^a,b   Golz, Stefan ^c   Korostileva, Kseniya A ^a,b   Vysotski, Eugene S ^a  

^a INSTITUTE OF BIOPHYSICS   (Russian Federation)

^b SIBERIAN FEDERAL UNIVERSITY   (Russian Federation)

^c BAYER PHARMA AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AEQUOREA VICTORIA; CLYTIA GREGARIA; HYDROZOA; MAMMALIA; SCYPHOZOA;

EID: 77952967129     PISSN: 1474905X     EISSN: 14749092     Source Type: Journal    
DOI: 10.1039/c0pp00023j     Document Type: Article

References (42)

1. O. Shimomura, Bioluminescence: Chemical Principles and Methods, World Scientific Publishing Co., Singapore, 2006
2. O. Shimomura F. H. Johnson Y. Saiga Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea J. Cell. Comp. Physiol. 1962 59 223 239
3. D. G. Allen J. R. Blinks F. G. Prendergast Aequorin luminescence: relation of light emission to calcium concentration-a calcium-independent component Science 1977 195 996 998
4. O. Shimomura F. H. Johnson Structure of the light-emitting moiety of aequorin Biochemistry 1972 11 1602 1608
5. M. J. Cormier K. Hori Y. D. Karkhanis J. M. Anderson J. E. Wampler J. G. Morin J. W. Hastings Evidence for similar biochemical requirements for bioluminescence among the coelenterates J. Cell. Physiol. 1973 81 291 297
6. 2+-regulated photoproteins: structural insight into the bioluminescence mechanism Acc. Chem. Res. 2004 37 405 415
7. J. G. Morin, in Coelenterate Biology: Reviews and New Perspectives, ed., L. Muscatine, and, H. M. Lenhoff, Academic Press, New York, 1974, pp. 397-438
8. J. G. Morin J. W. Hastings Energy transfer in a bioluminescent system J. Cell. Physiol. 1971 77 313 318
9. M. J. Cormier, in Bioluminescence in Action, ed., P. J. Herring, Academic Press, London, 1978, pp. 75-108
10. W. W. Ward, in Photochemical and Photobiological Reviews, ed., K. C. Smith, Plenum Press, New York, 1979, Vol. 4, pp. 1-57
11. P. J. Herring The spectral characteristics of luminous marine organisms Proc. R. Soc. London, Ser. B 1983 220 183 217
12. D. C. Prasher V. K. Eckenrode W. W. Ward F. G. Prendergast M. J. Cormier Primary structure of the Aequorea victoria green-fluorescent protein Gene 1992 111 229 233
13. N. S. Xia W. X. Luo J. Zhang X. Y. Xie H. J. Yang S. W. Li M. Chen M. H. Ng Bioluminescence of Aequorea macrodactyla, a common jellyfish species in the East China Sea Mar. Biotechnol. 2002 4 155 162
14. N. G. Gurskaya A. F. Fradkov N. I. Pounkova D. B. Staroverov M. E. Bulina Y. G. Yanushevich Y. A. Labas S. Lukyanov K. A. Lukyanov A colorless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutants Biochem. J. 2003 373 403 408
15. R. Y. Tsien The green fluorescent protein Annu. Rev. Biochem. 1998 67 509 544
16. R. Y. Tsien Creating new fluorescent probes for cell biology Nat. Rev. Mol. Cell Biol. 2002 3 906 918
17. Y. A. Labas N. G. Gurskaya Y. G. Yanushevich A. F. Fradkov K. A. Lukyanov S. A. Lukyanov M. V. Matz Diversity and evolution of the green fluorescent protein family Proc. Natl. Acad. Sci. U. S. A. 2002 99 4256 4261
18. 2+-activated photoprotein, clytin FEBS Lett. 1993 315 343 346 (Pubitemid 23025036)
19. S. Inouye Cloning, expression, purification and characterization of an isotype of clytin, a calcium-binding photoprotein from the luminous hydromedusa Clytia gregarium J. Biochem. 2008 143 711 717
20. W. W. Ward M. J. Cormier In vitro energy transfer in Renilla bioluminescence J. Phys. Chem. 1976 80 2289 2291
21. W. W. Ward M. J. Cormier Energy transfer via protein-protein interaction in Renilla bioluminescence Photochem. Photobiol. 1978 27 389 396
22. W. W. Ward M. J. Cormier An energy transfer protein in coelenterate bioluminescence. Characterization of the Renilla green fluorescent protein J. Biol. Chem. 1979 254 781 788
23. S. V. Markova, E. S. Vysotski and J. Lee, in Bioluminescence and Chemiluminescence 2000, ed., J. F. Case, P. J. Herring, B. H. Robison, S. H. D. Haddock, L. J. Kricka, and, P. E. Stanley, World Scientific, Singapore, 2001, pp. 115-118
24. B. A. Illarionov L. A. Frank V. A. Illarionova V. S. Bondar E. S. Vysotski J. R. Blinks Recombinant obelin: cloning and expression of cDNA, purification and characterization as a calcium indicator Methods Enzymol. 2000 305 223 249
25. E. S. Vysotski Z. J. Liu J. Rose B. C. Wang J. Lee Preparation and X-ray crystallographic analysis of recombinant obelin crystals diffracting to beyond 1.1 Å Acta Crystallogr., Sect. D: Biol. Crystallogr. 2001 57 1919 1921
26. M. E. Gasparian V. G. Ostapchenko A. A. Schulga D. A. Dolgih M. P. Kirpichikov Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli Protein Expression Purif. 2003 31 133 139
27. G. R. Fleming A. W. E. Knight J. M. Morris R. J. S. Morrison G. W. Robinson Picosecond fluorescence studies of xanthene dyes J. Am. Chem. Soc. 1977 99 4306 4311
28. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Third Edition, Springer, Singapore, 2006
29. N. C. Shaner P. A. Steinbach R. Y. Tsien A guide to choosing fluorescent proteins Nat. Methods 2005 2 905 909
30. E. M. Phizicky S. Fields Protein-protein interactions: Methods for detection and analysis Microbiol. Rev. 1995 59 94 123
31. J. Sambrook, E. F. Fritsch and T. Maniatis, Molecular Cloning: A Laboratory Manual, 2nd edn, Cold Spring Harbor Laboratory Press, NY, 1989
32. T. A. Brown, Essential Molecular Biology I: A Practical Approach Series, IRL Press, Oxford, 1990
33. S. Inouye M. Noguchi Y. Sakaki Y. Takagi T. Miyata S. Iwanaga T. Miyata F. I. Tsuji Cloning and sequence analysis of cDNA for the luminescent protein aequorin Proc. Natl. Acad. Sci. U. S. A. 1985 82 3154 3158
34. D. C. Prasher R. O. McCann M. Longiaru M. J. Cormier Sequence comparisons of complementary DNAs encoding aequorin isotypes Biochemistry 1987 26 1326 1332
35. O. Shimomura Isolation and properties of various molecular forms of aequorin Biochem. J. 1986 234 271 277
36. 2+-regulated photoproteins Biochemistry 2002 41 2227 2236
37. S. Inouye Y. Sahara Expression, purification and characterization of a photoprotein, clytin, from Clytia gregarium Protein Expression Purif. 2007 53 384 389
38. L. D. Levine W. W. Ward Isolation and characterization of a photoprotein "phialidin", and a spectrally unique green-fluorescent protein from the bioluminescent jellyfish Phialidium gregarium Comp. Biochem. Physiol 1982 72B 77 85
39. M. Chattoraj B. A. King G. U. Bublitz S. G. Boxer Ultra-fast excited state dynamics in green fluorescent protein: multiple states and proton transfer Proc. Natl. Acad. Sci. U. S. A. 1996 93 8362 8367
40. J. F. Head S. Inouye K. Teranishi O. Shimomura The crystal structure of the photoprotein aequorin at 2.3 Å resolution Nature 2000 405 372 376
41. 2+-regulated photoprotein obelin at 1.7Å resolution determined directly from its sulfur substructure Protein Sci. 2000 9 2085 2093
42. 2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state Proc. Natl. Acad. Sci. U. S. A. 2006 103 2570 2575