A colourless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutants

Biochemical Journal

Volumn 373, Issue 2, 2003, Pages 403-408

  Gurskaya, Nadya G ^a   Fradkov, Arkady F ^a   Pounkova, Natalia I ^a   Staroverov, Dmitry B ^b   Bulina, Maria E ^a   Yanushevich, Yurii G ^a   Labas, Yulii A ^c   Lukyanov, Sergey ^a   Lukyanov, Konstantin A ^a  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^b Evrogen JSC   (Russian Federation)

^c RUSSIAN ACADEMY OF SCIENCES   (Russian Federation)

Author keywords

Bioluminescence; Green fluorescent protein (GFP); Photoconversion; Protein labelling

Indexed keywords

CELLS; CLONING; FLUORESCENCE; ULTRAVIOLET RADIATION;

GREEN FLUORESCENT PROTEINS;

PROTEINS;

AEQUORIN; GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; BIOLUMINESCENCE; CELL MUTANT; DRUG ISOLATION; FLUORESCENCE; LABELING INDEX; MAMMAL CELL; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTUM MECHANICS;

ANIMALS; CELL LINE; CERCOPITHECUS AETHIOPS; COS CELLS; ESCHERICHIA COLI; FIBROBLASTS; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; RECOMBINANT FUSION PROTEINS; SCYPHOZOA; SPECTROMETRY, FLUORESCENCE; TRANSFECTION;

AEQUOREA; AEQUOREA COERULESCENS; AMPHIBIA; ANIMALIA; ESCHERICHIA COLI; HYDROIDA; HYDROMEDUSA; MAMMALIA; REPTILIA;

EID: 0041845246     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20021966     Document Type: Article

References (25)

1. Tsien, R. Y. (1998) The green fluorescent protein. Annu. Rev. Biochem. 67, 509-544
2. Johnson, F. H., Shimomura, O., Saiga, Y., Gershman, L. O., Reynolds, G. T. and Waters, J. R. (1962) Quantum efficiency of Cypridina luminescence, with a note on that of Aequorea. J. Cell. Comp. Physiol. 60, 85-104
3. Chalfie, M. (1995) Green fluorescent protein. Photochem. Photobiol. 62, 651-656
4. Matz, M. V., Fradkov, A. F., Labas, Y. A., Savitsky, A. P., Zaraisky, A. G., Markelov, M. L. and Lukyanov, S. A. (1999) Fluorescent proteins from nonbioluminescent Anthozoa species. Nat. Biotechnol. 17, 969-973
5. Lukyanov, K. A., Fradkov, A. F., Gurskaya, N. G., Matz, M. V., Labas, Y. A., Savitsky, A. P., Markelov, M. L., Zaraisky, A. G., Zhao, X., Fang, Y. et al. (2000) Natural animal coloration can be determined by a non-fluorescent green fluorescent protein homolog. J. Biol. Chem. 275, 25879-25882
6. Dove, S. G., Hoegh-Gurdberg, O. and Ranganathan, S. (2001) Major colour patterns of reef-buirding corals are due to a family of GFP-like proteins. Coral Reefs 19, 197-204
7. Gurskaya, N. G., Fradkov, A. F., Terskikh, A., Matz, M. V., Labas, Y. A., Martynov, V. I., Yanushevich, Y. G., Lukyanov, K. A. and Lukyanov, S. A. (2001) GFP-like chromoproteins as a source of far-red fluorescent proteins. FEBS Lett. 507, 16-20
8. Salih, A., Larkum, A., Cox, G., Kuhl, M. and Hoegh-Gurdberg, O. (2000). Fluorescent pigments in corals are photoprotective. Nature (London) 408, 850-853
9. Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A. and Matz, M. V. (2002) Diversity and evolution of the green fluorescent protein family. Proc. Natl. Acad. Sci. U.S.A. 99, 4256-4261
10. Matz, M. V., Lukyanov, K. A. and Lukyanov, S. A. (2002) Family of the green fluorescent protein: journey to the end of the rainbow. BioEssays 24, 953-959
11. Matz, M., Shagin, D., Bogdanova, E., Britanova, O., Lukyanov, S., Diatchenko, L. and Chenchik, A. (1999) Amplification of cDNA ends based on template-switching effect and step-out POR. Nucleic Acids Res. 27, 1558-1560
12. Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K. and Pease, L. R. (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51-59
13. Ward, W. W. (1981) Properties of the coelenterate green-fluorescent protein. In Bioluminescence and Chemiluminescence (DeLuca, M. and McElroy, W., eds.), pp. 235-242, Academic Press, New York
14. Patterson, G., Day, R. N. and Piston, D. (2001) Fluorescent protein spectra. J. Cell Sci. 114, 837-838
15. Kramp, P. L. (1968) The hydromedusae of the Pacific and Indian Oceans. Dana Rept. 72, 201-202
16. Pogodin, A. G. and Yakovlev, Yu. M. (1999) First record of hydroid medusae of the genus Aequorea in Russian waters of the Sea of Japan. Rus. J. Mar. Biol 25, 417-419
17. Baird, G. S., Zacharias, D. A. and Tsien, R. Y. (2000). Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proc. Natl. Acad. Sci. U.S.A. 97, 11984-11989
18. Mach, H., Middaugh, C. R. and Lewis, R. V. (1992) Statistical determination of the average values of the extinction coefficients of tryptophan and tyrosine in native proteins. Anal. Biochem. 200, 74-80
19. Cubitt, A. B., Heim, R., Adams, S. R., Boyd, A. E., Gross, L. A. and Tsien, R. Y. (1995) Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20, 448-455
20. Brejc, K., Sixma, T. K., Kitts, P. A., Kain, S. R., Tsien, R. Y., Ormo, M. and Remington, S. J. (1997) Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein. Proc. Natl. Acad. Sci. U.S.A 94, 2306-2311
21. van Thor, J. J., Gensch, T., Hellingwerf, K. J. and Johnson, L. N. (2002) Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222. Nat. Struct. Biol. 9, 37-41
22. Patterson, G. H. and Lippincott-Schwartz, J. (2002) A photoactivatable GFP for selective photolabeling of proteins and cells. Science (Washington, D.C.) 297, 1873-1877
23. Yarbrough, D., Wachter, R. M., Kallio, K., Matz, M. V. and Remington, S. J. (2001) Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-Å, resolution. Proc. Natl. Acad. Sci. U.S.A. 98, 462-467
24. Heim, R., Cubitt, A. B. and Tsien, R. Y. (1995) Improved green fluorescence. Nature (London) 373, 663-864
25. Ehrig, T., O'Kane, D. J. and Prendergast, F. G. (1995) Green-fluorescent protein mutants with altered fluorescence excitation spectra. FEBS Lett. 367, 163-166