Effect of the abrogation of TGF-β1 by antisense oligonucleotides on the expression of TGF-β-isoforms and their receptors I and II in isolated fibroblasts from keloid scars

International Journal of Molecular Medicine

Volumn 25, Issue 6, 2010, Pages 915-921

  Bran, Gregor M ^a   Goessler, Ulrich R ^a   Schardt, Christopher ^a   Hormann, Karl ^a   Riedel, Frank ^a   Sadick, Haneen ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Antisense; Fibroblast; Keloid; Transforming growth factor isoform; Transforming growth factor receptor

Indexed keywords

ANTISENSE OLIGODEOXYNUCLEOTIDE; MESSENGER RNA; TRANSFORMING GROWTH FACTOR BETA RECEPTOR 2; TRANSFORMING GROWTH FACTOR BETA1; TRANSFORMING GROWTH FACTOR BETA2; TRANSFORMING GROWTH FACTOR BETA3;

ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FIBROBLAST; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; KELOID; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA SYNTHESIS;

EID: 77952786594     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm_00000422     Document Type: Article

References (34)

1. Tuan TL and Nichter LS: The molecular basis of keloid and hypertrophic scar formation. Mol Med Today 4: 19-24, 1998.
2. Bran GM, Goessler UR, Hormann K, Riedel F and Sadick H: Keloids: current concepts of pathogenesis (Review). Int J Mol Med 24: 283-293, 2009.
3. Bock O, Yu H, Zitron S, Bayat A, Ferguson MW and Mrowietz U: Studies of transforming growth factors beta 1-3 and their receptors I and II in fibroblasts of keloids and hypertrophic scars. Acta Dermatol Venereol 85: 216-220, 2005. (Pubitemid 40695163)
4. Chin GS, Liu W, Peled Z, Lee TY, Steinbrech DS, Hsu M and Longaker MT: Differential expression of transforming growth factor-beta receptors I and II and activation of Smad 3 in keloid fibroblasts. Plast Reconstr Surg 108: 423-429, 2001. (Pubitemid 32706075)
5. Al-Attar A, Mess S, Thomassen JM, Kaufmann CL and Davison SP: Keloid pathogenesis and treatment. Plast Reconstr Surg 117: 286-300, 2006.
6. Robles DT, Moore E, Draznin M and Berg D: Keloids: Pathophysiology and management. Dermatol Online J 13: 9, 2007.
7. Bayat A, Arscott G, Ollier WE, Ferguson MW and McGrouther DA: 'Aggressive keloid': a severe variant of familial keloid scarring. J R Soc Med 96: 554-555, 2003. (Pubitemid 37510429)
8. Mustoe TA, Cooter RD, Gold MH, Hobbs R, Ramelet AA, Shakespeare PG, Stella M, et al: International clinical recommendations on scar management. Plast Reconstr Surg 110: 560-571, 2002. (Pubitemid 34815962)
9. Jagadeesan J and Bayat A: Transforming growth factor beta (TGFβ) and keloid disease. Int J Surg 5: 278-285, 2007. (Pubitemid 47095482)
10. Assoian RK, Komoriya A, Meyers CA, Miller DM and Sporn MB: Transforming growth factor-beta in human platelets. Identification of a major storage site, purification and characterization. J Biol Chem 258: 7155-7160, 1983. (Pubitemid 13059967)
11. Lee TY, Chin GS, Kim WJ, Chau D, Gittes GK and Longaker MT: Expression of transforming growth factor beta 1, 2, and 3 proteins in keloids. Ann Plast Surg 43: 179-184, 1999.
12. Sadick H, Herberger A, Riedel K, Bran G, Goessler U, Hormann K and Riedel F: TGF-β1 antisense therapy modulates expression of matrix metalloproteinases in keloid-derived fibroblasts. Int J Mol Med 22: 55-60, 2008.
13. Butler PD, Longaker MT and Yang GP: Current progress in keloid research and treatment. J Am Coll Surg 206: 731-741, 2008.
14. Massague J: TGFbeta signaling: receptors, transducers and Mad proteins. Cell 85: 947-950, 1996.
15. Bettinger DA, Yager DR, Diegelmann RF and Cohen IK: The effect of TGF-beta on keloid fibroblast proliferation and collagen synthesis. Plast Reconstr Surg 98: 827-833, 1996.
16. Polo M, Smith PD, Kim YJ, Wang X, Ko F and Robson MC: Effect of TGF-beta on proliferative scar fibroblast kinetics. Ann Plast Surg 43: 185-190, 1999.
17. Hernandez A and Evers BM: Functional genomics: clinical effect and the evolving role of the surgeon. Arch Surg 134: 1209-1215, 1999.
18. Ignotz RA and Massague J: Transforming growth factor beta stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix. J Biol Chem 261: 4337-4345, 1986.
19. Overall CM, Wrana J and Sodek J: Independent regulation of collagenase, 72 kd-progelatinase, and metalloproteinase inhibitor (TIMP) expression in human fibroblasts by transforming growth factor-beta. J Biol Chem 264: 1860-1869, 1989.
20. Border WA, Okuda S, Langiuno LR, Sporn MB and Ruoslathi E: Supression of experimental glomerulonephritis by antiserum against transforming growth factor beta 1. Nature 346: 371-374, 1990.
21. Castilla A, Prieto J and Fausto N: Transforming growth factor beta and alpha in chronic liver disease. N Engl J Med 324: 933-940, 1991.
22. Broekelmann TJ, Limper AH, Colby TV and McDonald JA: Transforming growth factor beta 1 is present at sites of extracellular matrix gene expression in human pulmonary fibrosis. Proc Nat Acad Sci USA 88: 6642-6646, 1991.
23. Tredget EE: The molecular biology of fibroproliferative disorders of the skin: potential cytokine therapeutics. Ann Plast Surg 33: 152-154, 1994.
24. Younai S, Nichter LS, Wellisz T, Reinisch J, Nimni ME and Tuan TL: Modulation of collagen synthesis by transforming growth factor beta in keloid and hypertrophic scar fibroblasts. Ann Plast Surg 33: 148-154, 1994.
25. Ogawa Y, Ksander GA, Pratt BM, Sawamura SJ, Ziman JM, Gerhardt CO, Avis PD, et al: Differences in the biological activities of transforming growth factor-beta and platelet-derived growth factor in vivo. Growth factors 5: 57-68, 1991.
26. Shah M, Foreman DM and Ferguson MW: Neutralisation of TGF-beta1 and TGF-beta2 or exogenous addition of TGF-beta3 to cutaneous wounds reduces scarring. J Cell Sci 108: 985-1002, 1995.
27. Bissel DM, Roulot D and George J: Transforming growth factor beta and the liver. Hepatology 34: 859-867, 2001.
28. Derynck R and Zhang Y: Intracellular signaling: the mad way to do it. Curr Biol 6: 1226-1229, 1996.
29. Leask A and Abraham DJ: TGF-beta signaling and the fibrotic response. FASEB J 18: 816-827, 2004.
30. Goldberg HJ, Huszar T, Mozes MM, Rosivall L and Musci I: Overexpression of the type II transforming growth factor-beta receptor inhibits fibroblast proliferation and activates extracellular signal regulated kinase and c-Jun N-terminal kinase. Cell Biol Int 26: 165-174, 2002.
31. Yamamoto H, Ueno H, Ooshima A and Takeshita A: Adenovirus- mediated transfer of a truncated transforming growth factor-beta (TGF-beta) type II receptor completely and specifically abolishes diverse signaling by TGF-beta in vascular wall cells in primary culture. J Biol Chem 271: 16253-16259, 1996.
32. Chu Y, Guo F, Li Y, Li X, Zhou T and Guo Y: A novel truncated TGF-beta receptor II downregulates collagen synthesis and TGF-beta I secretion of keloid fibroblasts. Connect Tissue Res 49: 92-98, 2008.
33. Centrella M, Casinghino S, Kim J, Pham T, Rosen V, Wozney J and McCarthy TL: Independent changes in type I and type II receptors for transforming growth factor beta induced by bone morphogenic protein 2 parallel expression of the osteoblast phenotype. Mol Cell Biol 25: 3273-3278, 1995.
34. Mori Y, Hatamochi A, Arakawa M and Ueki H: Reduced expression of mRNA for transforming growth factor beta (TGFbeta) and TGFbeta receptors I and II and decreased TGFbeta binding to the receptors in in vitro-aged fibroblasts. Arch Dermatol Res 290: 158-162, 1997.