Comparative evaluation of INNO-LiPA HBV assay, direct DNA sequencing and subtractive PCR-RFLP for genotyping of clinical HBV isolates

Virology Journal

Volumn 7, Issue 1, 2010, Pages

  Ali, Maisa M ^a,c   Hasan, Fuad ^a,b,c   Ahmad, Suhail ^a,c   Al Nakib, Widad ^a,c  

^a KUWAIT UNIVERSITY   (Kuwait)

^b MUBARAK AL KABEER HOSPITAL   (Kuwait)

^c DASMAN DIABETES INSTITUTE   (Kuwait)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA; VIRUS PROTEIN;

ARTICLE; ASSAY; BLOOD SAMPLING; CHEMISTRY; CLASSIFICATION; COMPARATIVE STUDY; CONTROLLED STUDY; DNA PROBE; DNA SEQUENCE; GENETICS; GENOTYPE; HEPATITIS B; HEPATITIS B VIRUS; HEPATITIS B VIRUS GENOTYPE A; HEPATITIS B VIRUS GENOTYPE D; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR GENETICS; NONHUMAN; NUCLEIC ACID HYBRIDIZATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RESTRICTION SITE; REVERSE HYBRIDIZATION BASED LINE PROBE ASSAY; SEQUENCE ALIGNMENT; VIROLOGY; VIRUS GENOME; VIRUS STRAIN;

BASE SEQUENCE; CHEMISTRY; CLASSIFICATION; DNA PROBES; DNA, VIRAL; GENETICS; GENOTYPE; HEPATITIS B; HEPATITIS B VIRUS; HUMANS; ISOLATION AND PURIFICATION; METHODS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; VIRAL PROTEINS; VIROLOGY;

EID: 77952702718     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/1743-422X-7-111     Document Type: Article

References (20)

1. Norder H, Courouce A, Coursaget P, Echevarria JM, Lee SD, Mushahwar IK, Robertson BH, Locarnini S, Magnius LO: Genetic diversity of hepatitis B virus strains derived worldwide: genotypes, subgenotypes and HBsAg subtypes. Intervirology 2004, 47:289-309.
2. Schaefer S: Hepatitis B virus: significance of genotypes. J Viral Hepat 2005, 12:111-124.
3. Baumert T, Thimme R, Weizsacher F: Pathogenesis of hepatitis B virus infection. World J Gastroenterol 2007, 13:82-90.
4. But D, Lai C, Yuen M: Natural history of hepatitis-related hepatocellular carcinoma. World J Gastroenterol 2008, 14:1652-1656.
5. Sumi H, Yokosuka O, Seki N, Arai M, Imazeki F, Kurihara T, Kanda T, Fukai K, Kato M, Saisho H: Influence of hepattis B virus genotypes on the progression of chronic type B liver disease. Hepatology 2003, 37:19-26.
6. Bartholomeusz A, Schaefer S: Hepatitis B virus genotypes: comparison of genotyping methods. Rev Med Virol 2004, 14:3-16.
7. Kato H, Ruzibakiev R, Yuldasheva N, Hegay T, Kurbanov F, Achunfjanov B, Tuichiev L, Usuda S, Ueda R, Mizokami M: Hepatitis B virus genotypes in Uzbekistan and validity of two different systems for genotyping. J Med Virol 2002, 67:477-483.
8. Mizokami M, Nakano T, Orito E, Tanaka Y, Sakugawa H, Mukaide M, Robertson BH: Hepatitis B virus genotype assignment using restriction fragment length polymorphism patterns. FEBS Lett 1999, 450:66-71.
9. Osiowy C, Giles E: Evaluation of the INNO-LiPA HBV genotyping assay for determination of hepatitis B virus genotype. J Clin Microbiol 2003, 41:5473-7.
10. Novoa SR, Tato AG, Guirao AA, Castroagudin J, Quintela AG, Riestra CG, Regueiro B: Hepatitis B virus genotyping based on cluster analysis of the region involved in lamivudine resistance. J Virol Meth 2004, 115:9-17.
11. Ahmad S, Ali MM, Mustafa AS: Construction of a modified vector for efficient purification of recombinant Mycobacterium tuberculosis proteins expressed in Escherichia coli. Protein Expr Purif 2003, 29:167-175.
12. Ahmad S, Glavas NA, Bragg PD: A mutation at the Gly314 of the β subunit of the Escherichia coli pyridine nucleotide transhydrogenase abolishes activity and affects the NADP(H)-induced conformational change. Eur J Biochem 1992, 207:733-739.
13. Ahmad S, Mokaddas E, Jaber AA: Rapid detection of ethambutolresistant Mycobacterium tuberculosis strains by PCR-RFLP targeting embB codons 306 and 497 and iniA codon 501 mutations. Mol Cell Probes 2004, 18:299-306.
14. Dalwai A, Ahmad S, Pacsa A, Al-Nakib W: Echovirus type 9 is an important cause of viral encephalitis among infants and children in Kuwait. J Clin Virol 2009, 44:48-51.
15. Chen B, Chen P, Jow G, Sablon E, Liu CJ, Chen DS, Kao JH: High prevalence of mixed genotype infections in hepatitis B virus infected intravenous drug users. J Med Virol 2004, 74:536-542.
16. Grandjacques C, Pradat P, Stuyver L, Chevallier M, Chevallier P, Pichoud C, Maisonnas M, Trépo C, Zoulim F: Rapid detection of genotypes and mutations in the pre-core promoter and the pre-core region of hepatitis B virus genome: correlation with viral persistence and disease severity. J Hepatol 2000, 33:430-9.
17. Amini-Bavil-Olyaee S, Sarrami-Forooshani R, Adeli A, Sabahi F, Abachi M, Azizi M, Mahboudi F: Complete Genomic Sequence and Phylogenetic Relatedness of Hepatitis B Virus Isolated from Iran. J Med Virol 2005, 76:318-326.
18. Saudy N, Sugauchi F, Tanaka Y, Suzuki S, Aal AA, Zaid MA, Agha S, Mizokami M: Genotypes and phylogenetic characterization of hepatitis B and delta viruses in Egypt. J Med Virol 2003, 70:529-36.
19. Sunbul M, Leblebicioglu H: Distribution of hepatitis B virus genotypes in patients with chronic hepatitis B in Turkey. World J Gastroenterol 2005, 11:1976-1980.
20. Magnius L, Norder H: Subtypes, genotypes and molecular epidemiology of the hepatitis B virus as reflected by sequence variability of the S-gene. Intervirology 1995, 38:24-34.