A non-isotopic assay uses bromouridine and RNA synthesis to detect DNA damage responses

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 699, Issue 1-2, 2010, Pages 62-66

  Hasegawa, Mayu ^a   Iwai, Shigenori ^a   Kuraoka, Isao ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Bromouridine; DNA damage; Transcription inhibition; Transcription coupled repair

Indexed keywords

BROMOURIDINE; DNA DIRECTED RNA POLYMERASE; RNA; RNA POLYMERASE II; TRITIUM; UNCLASSIFIED DRUG; URIDINE; URIDINE DERIVATIVE;

ARTICLE; CELL NUCLEUS; CONTROLLED STUDY; DNA DAMAGE; FLUORESCENCE MICROSCOPY; HELA CELL; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; NUCLEOLUS; PRIORITY JOURNAL; RNA SYNTHESIS; RNA TRANSCRIPTION;

BIOLOGICAL ASSAY; CELL LINE; COCKAYNE SYNDROME; DNA DAMAGE; DNA REPAIR; HELA CELLS; HUMANS; MICROSCOPY, FLUORESCENCE; RNA; RNA POLYMERASE I; RNA POLYMERASE II; ULTRAVIOLET RAYS; URIDINE; XERODERMA PIGMENTOSUM;

EID: 77952582649     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2010.04.002     Document Type: Article

References (24)

1. Friedberg E.C., Walker G.C., Siede W., Wood R.D., Schultz R.A., and Ellenberger T. DNA Repair and Mutagenesis (2005), American Society for Microbiology, Washington, DC
2. Svejstrup J.Q. Mechanisms of transcription-coupled DNA repair. Nat. Rev. Mol. Cell Biol. 3 (2002) 21-29
3. Hanawalt P.C., Donahue B.A., and Sweder K.S. Repair and transcription. Collision or collusion?. Curr. Biol. 4 (1994) 518-521
4. Saijo M., Hirai T., Ogawa A., Kobayashi A., Kamiuchi S., and Tanaka K. Functional TFIIH is required for UV-induced translocation of CSA to the nuclear matrix. Mol. Cell. Biol. 27 (2007) 2538-2547
5. Nance M.A., and Berry S.A. Cockayne syndrome: review of 140 cases. Am. J. Med. Genet. 42 (1992) 68-84
6. Groisman R., Kuraoka I., Chevallier O., Gaye N., Magnaldo T., Tanaka K., Kisselev A.F., Harel-Bellan A., and Nakatani Y. CSA-dependent degradation of CSB by the ubiquitin-proteasome pathway establishes a link between complementation factors of the Cockayne syndrome. Genes Dev. 20 (2006) 1429-1434
7. Horibata K., Iwamoto Y., Kuraoka I., Jaspers N.G.J., Kurimasa A., Oshimura M., Ichihashi M., and Tanaka K. Complete absence of Cockayne syndrome group B gene product gives rise to UV-sensitive syndrome but not Cockayne syndrome. Proc. Natl. Acad. Sci. U.S.A. 101 (2004) 15410-15415
8. Venema J., Vanhoffen A., Karcagi V., Natarajan A.T., Vanzeeland A.A., and Mullenders L.H.F. Xeroderma-pigmentosum complementation group-C cells remove pyrimidine dimers selectively from the transcribed strand of active genes. Mol. Cell. Biol. 11 (1991) 4128-4134
9. Kuraoka I. Effects of DNA lesions on transcription elongation by RNA polymerases. Genes Environ. 30 (2008) 63-70
10. Donahue B.A., Yin S., Taylor J.S., Reines D., and Hanawalt P.C. Transcript cleavage by RNA polymerase II arrested by a cyclobutane pyrimidine dimer in the DNA template. Proc. Natl. Acad. Sci. U.S.A. 91 (1994) 8502-8506
11. Nakatsu Y., Asahina H., Citterio E., Rademakers S., Vermeulen W., Kamiuchi S., Yeo J.P., Khaw M.C., Saijo M., Kodo N., Matsuda T., Hoeijmakers J.H.J., and Tanaka K. XAB2, a novel tetratricopeptide repeat protein involved in transcription-coupled DNA repair and transcription. J. Biol. Chem. 275 (2000) 34931-34937
12. Itoh T., Yamaizumi M., Ichihashi M., HiroOka M., Matsui T., MAtsuno M., and Ono T. Clinical characteristics of three patients with UVs syndrome, a photosensitive disorder with defective DNA repair. Br. J. Dermatol. 134 (1996) 1147-1150
13. Diermeier S., Schmidt-Bruecken E., Kubbies M., Kunz-Schughart L.A., and Brockhoff G. Exposure to continuous bromodeoxyuridine (BrdU) differentially affects cell cycle progression of human breast and bladder cancer cell lines. Cell Prolif. 37 (2004) 195-206
14. Hashimoto S., Egawa K., Ihn H., and Tateishi S. Non-radioisotope method for diagnosing photosensitive genodermatoses and a new marker for xeroderma pigmentosum variant. J. Dermatol. 36 (2009) 138-143
15. Thiry M., Lamaye F., Thelen N., Chatron-Colliet A., Lalun N., Bobichon H., and Ploton D. A protocol for studying the kinetics of RNA within cultured cells: application to ribosomal RNA. Nat. Protoc. 3 (2008) 1997-2004
16. Limsirichaikul S., Niimi A., Fawcett H., Lehmann A., Yamashita S., and Ogi T. A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU). Nucleic Acids Res. 37 (2009)
17. Salic A., and Mitchison T.J. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc. Natl. Acad. Sci. U.S.A. 105 (2008) 2415-2420
18. Koberna K., Landa V., Kanka J., Pliss A., Eltsov M., Stanek D., and Raska I. Non-isotopic detection of nucleolar transcription in pre-implantation mouse embryos. Reprod. Nutr. Dev. 38 (1998) 117-126
19. Brueckner F., Hennecke U., Carell T., and Cramer P. CPD damage recognition by transcribing RNA polymerase II. Science 315 (2007) 859-862
20. Groisman R., Polanowska J., Kuraoka I., Sawada J., Saijo M., Drapkin R., Kisselev A.F., Tanaka K., and Nakatani Y. The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage. Cell 113 (2003) 357-367
21. Bradsher J., Auriol J., de Santis L.P., Iben S., Vonesch J.L., Grummt I., and Egly J.M. CSB is a component of RNA pol I transcription. Mol. Cell 10 (2002) 819-829
22. Waksmundzka M., and Debey P. Electric field-mediated BrUTP uptake by mouse oocytes, eggs, and embryos. Mol. Reprod. Dev. 58 (2001) 173-179
23. Koberna K., Stanek D., Malinsky J., Eltsov M., Pliss A., Ctrnacta V., Cermanova S., and Raska I. Nuclear organization studied with the help of a hypotonic shift: its use permits hydrophilic molecules to enter into living cells. Chromosoma 108 (1999) 325-335
24. Kuraoka I., Ito S., Wada T., Hayashida M., Lee L., Saijo M., Nakatsu Y., Matsumoto M., Matsunaga T., Handa H., Qin J., Nakatani Y., and Tanaka K. Isolation of XAB2 complex involved in pre-mRNA splicing, transcription, and transcription-coupled repair. J. Biol. Chem. 283 (2008) 940-950