Effect of high pH column regeneration on the separation performances in reversed phase chromatography of peptides

Journal of Chromatography A

Volumn 1217, Issue 21, 2010, Pages 3531-3537

  Gétaz, David ^a   Gencoglu, Mumun ^a   Forrer, Nicola ^b   Morbidelli, Massimo ^a  

^a ETH ZURICH   (Switzerland)

^b NONE   (Switzerland)

Author keywords

Chromatography; Cleaning in place; Peptide; Reversed phase; Silanol activity

Indexed keywords

ANALYTES; CHROMATOGRAPHIC PURIFICATION; CLEANING IN PLACE; ELECTROSTATIC INTERACTIONS; HIGH PH; PEPTIDE ADSORPTION; POSITIVELY CHARGED; PROTONATED; PURIFICATION PROCESS; REVERSED PHASE; REVERSED PHASE CHROMATOGRAPHY; SEPARATION PERFORMANCE; SILANOL ACTIVITY; SILANOL GROUPS; SILANOLS; STATIONARY PHASE;

ADSORPTION; CHEMICAL CLEANING; CHEMICAL STABILITY; COLUMN CHROMATOGRAPHY; IMPURITIES; PEPTIDES; PURIFICATION; SILICA; SURFACE CHEMISTRY; SURFACE CLEANING;

PH EFFECTS;

ACETONITRILE; BENZYLAMINE; PHENOL; PROTON; SILANOL; SILICON DIOXIDE; TRIETHYLAMINE;

ADSORPTION; ARTICLE; CONTROLLED STUDY; ELUTION; HYDROPHOBICITY; PEPTIDE ANALYSIS; PH; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTON TRANSPORT; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE; STATIC ELECTRICITY; SURFACE PROPERTY;

ACETONITRILES; CHROMATOGRAPHY, REVERSE-PHASE; EQUIPMENT REUSE; ETHYLAMINES; HYDROGEN-ION CONCENTRATION; HYDROPHOBICITY; OSMOLAR CONCENTRATION; PEPTIDES; SILANES;

EID: 77952550881     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2010.03.042     Document Type: Article

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