Simplified method to measure glucocorticoid metabolites in faeces of horses

Veterinary Research Communications

Volumn 34, Issue 2, 2010, Pages 185-195

  Flauger, Birgit ^a   Krueger, Konstanze ^a   Gerhards, Hartmut ^b   Möstl, Erich ^c  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

^c UNIVERSITY OF VETERINARY MEDICINE   (Austria)

Author keywords

ACTH challenge; Cortisol; Enzyme immunoassay; Stress behavior

Indexed keywords

CORTICOTROPIN; DEXAMETHASONE; DRUG METABOLITE; ETIOCHOLANOLONE; HYDROCORTISONE; METHANOL;

ACCURACY; ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; CORTICOTROPIN TEST; DRUG DETERMINATION; ENZYME IMMUNOASSAY; FECES ANALYSIS; FEMALE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HORSE; IMMUNOREACTIVITY; MALE; NONHUMAN; ROOM TEMPERATURE; STORAGE TEMPERATURE; SUPERNATANT; VETERINARY MEDICINE; WILDLIFE CONSERVATION;

ADRENOCORTICOTROPIC HORMONE; ANIMALS; DEXAMETHASONE; ETIOCHOLANOLONE; FECES; FEMALE; GLUCOCORTICOIDS; HORSES; IMMUNOENZYME TECHNIQUES; MALE; STRESS, PHYSIOLOGICAL;

EQUIDAE;

EID: 77952542974     PISSN: 01657380     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11259-010-9344-y     Document Type: Article

References (21)

1. Alexander S.L. and Irvine C.H.G. (1998) The effect of social stress on adrenal axis activity in horses: the importance of monitoring corticosteroid-binding globulin capacity. J Endocrinol 157:425-432.
2. Bosson C.O., Palme R., Boonstra R. (2009) Assessment of the stress response in columbian ground squirrels: laboratory and field validation of an enzyme immunoassay for fecal cortisol metabolites. Physiol Biochem Zool 82:291-301.
3. Cannon W.B. (1935) Stresses and strains of homeostasis. Am J Med Sci 189:1-14.
4. Ganswindt A., Palme R., Heistermann M., Borragan S., Hodges J.K. (2003) Non-invasive assessment of adrenocortical function in the male African elephant (Loxodonta Africana) and its relation to musth. Gen Comp Endocrinol 134:156-166.
5. Gorgasser I., Tichy A., Palme R. (2007) Faecal cortisol metabolites in Quarter Horses during initial training under field conditions. Vet Med Austria, Wien Tierärztl Mschr 94:226-230.
6. Heistermann M., Palme R., Ganswindt A. (2006) Comparison of different enzymeimmunoassays for the assessment of adrenocortical activity in primates based on fecal analysis. Am J Primatol 68:257-273.
7. Hopster H., van der Werf J.T.N., Erkens J.H.F., Blokhuis H.J. (1999) Effects of repeated jugular puncture on plasma cortisol concentration in loose-housed dairy cows. J Anim Sci 77:708-714.
8. Kohen F., Bauminger S., Lindner H.R. (1975) Preparation of antigenic steroid-protein conjugates. In: Cameron E.H.D., Hillier S.G., Griffiths K. (eds) Steroid Immunoassays. Omega Publishing, Cardiff, pp 11-32.
9. Matteri R.L., Carroll J.A., Dyer D.J. (2000) Neuroendocrine responses to stress. In: Moberg J.P. and Mench J.A. (eds) The Biology of Animal Stress. CABI Publishing, Oxford, pp 43-76.
10. Merl S., Scherzer S., Palme R., Möstl E. (2000) Pain causes increased concentrations of glucocorticoid metabolites in equine faeces. J Equine Vet Sci 20:586-590.
11. Moons C., Heleski C.R., Leece C.M., Zanella A.J. (2002) Conflicting results in the association between plasma and salivary cortisol levels in foals. Harvemeyer Foundation Workshop, Iceland.
12. Möstl E. and Palme R. (2002) Hormones as indicators of stress. Domest Anim Endocrinol 23:67-74.
13. Möstl E., Messmann S., Bagu E., Robia C., Palme R. (1999) Measurement of glucocorticoid metabolite concentration in faeces of domestic livestock. J Vet Med A 46:621-631.
14. Möstl E., Maggs J.L., Schrötter G., Besenfelder U., Palme R. (2002) Measurement of cortisol etabolites in faeces of ruminants. Vet Res Commun 26:127-139.
15. Niswender G.D. and Midgley A.R.Jr. (1970) In: Peron F.G. and Caldwell B.V. (eds) Immunologic Methods in Steroid Determination. Appleton-Century- Crofts, New York, pp 149-173.
16. Palme R. and Möstl E. (1997) Measurement of cortisol metabolites in faeces of sheep as a parameter of cortisol concentration in blood. Int J Mamm Biol 62:192-197.
17. Palme R., Fischer P., Schildorfer H., Ismail M.N. (1996) Excretion of 14C-steroid hormones via faeces and urine in domestic livestock. Anim Reprod Sci 43:43-63.
18. Sapolsky R.M., Romero L.M., Munck A.U. (2000) How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory and preparative actions. Endocr Rev 21:55-89.
19. Selye H.A. (1936) A syndrome produced by diverse nocuous agents. Nature 138:32-33.
20. Touma C. and Palme R. (2005) Measuring fecal glucocorticoid metabolites in mammals and birds: the importance of validation. Ann N Y Acad Sci 1046:54-74.
21. Wingfield J.C. and Ramenofsky M. (1999) Hormones and the behavioural ecology of stress. In: Balm P.H.M. (ed) Stress Physiology in Animals. Sheffield Academic Press, pp 1-51.