Integration of stable extracellular DNA released from Escherichia coli into the Bacillus subtilis genome vector by culture mix method

Nucleic Acids Research

Volumn 38, Issue 8, 2010, Pages 2551-2557

  Itaya, Mitsuhiro ^a,b   Kaneko, Shinya ^a,c  

^a MITSUBISHI KAGAKU INSTITUTE OF LIFE SCIENCES   (Japan)

^b KEIO UNIVERSITY   (Japan)

^c TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID DNA;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENOME; BACTERIOPHAGE LAMBDA; BIOTRANSFORMATION; CELL LYSATE; CONTROLLED STUDY; CULTURE MEDIUM; DNA INTEGRATION; DNA PURIFICATION; DNA TRANSFER; ESCHERICHIA COLI; GENE VECTOR; MIXED CELL CULTURE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; BACTERIAL ARTIFICIAL CHROMOSOME; BACTERIUM TRANSFORMATION; GENETICS; METHODOLOGY; PLASMID;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

BACILLUS SUBTILIS; CHROMOSOMES, ARTIFICIAL, BACTERIAL; CLONING, MOLECULAR; CULTURE MEDIA; ESCHERICHIA COLI; GENETIC VECTORS; GENOME, BACTERIAL; PLASMIDS; TRANSFORMATION, BACTERIAL;

EID: 77952492811     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkq142     Document Type: Article

References (18)

1. Carr, P.A. and Church, G.M. (2009) Genome engineering. Nat. Biotechnol., 27, 1151-1162.
2. Itaya, M., Tsuge, K., Koizumi, M. and Fujita, K. (2005) Combining two genomes in one cell: stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome. Proc. Natl Acad. Sci. USA, 102, 15971-15976.
3. Lartigue, C., Vashee, S., Algire, M.A., Chuang, R.Y., Bender, G.A., Ma, L., Noskov, V.N., Denisova, E.A., Gibson, D.G., Assad-Garcia, N. et al. (2009) Creating bacterial strains from genomes that have been cloned and engineered in yeast. Science, 325, 1693-1696.
4. Gibson, D.G., Benders, G.A., Andrews-Pfannkoch, C., Denisova, E.A., Baden-Tillson, H., Zaveri, J., Stockwell, T.B., Brownley, A., Thomas, D.W., Algire, M.A. et al. (2008) Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome. Science, 319, 1215-1220.
5. Itaya, M. (2009) Recombinant genomes. In Fu, P. and Panke, S. (eds), Systems Biology and Synthetic Biology. John Wiley & Sons, Inc., Hoboken, NJ, pp. 155-192.
6. Itaya, M., Nagata, T., Shiroishi, T., Fujita, K. and Tsuge, K. (2000) Efficient cloning and engineering of giant DNAs in a novel Bacillus subtilis genome vector. J. Biochem., 128, 869-875.
7. Kaneko, S., Tsuge, K., Takeuchi, T. and Itaya, M. (2003) Conversion of submegasized DNA to desired structures using a novel Bacillus subtilis genome vector. Nucleic Acids Res., 31, e112.
8. Kaneko, S., Akioka, M., Tsuge, K. and Itaya, M. (2005) DNA shuttling between plasmid vectors and a genome vector: systematic conversion and preservation of DNA libraries using the Bacillus subtilis genome (BGM) vector. J. Mol. Biol., 349, 1036-1044.
9. Itaya, M., Fujita, K., Kuroki, A. and Tsuge, K. (2008) Bottom-up genome assembly using the Bacillus subtilis genome vector. Nat. Methods, 5, 41-43.
10. Kaneko, S., Takeuchi, T. and Itaya, M. (2009) Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector. J. Biotech., 139, 211-213.
11. Kaneko, S. and Itaya, M. Designed horizontal transfer of stable giant DNA released from Escherichia coli. J. Biochem., doi:101093/jb/mvq012 [9 February 2010, Epub ahead of print].
12. Sambrook, J., Fritsch, E.C. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
13. Itaya, M. and Tanaka, T. (1991) Complete physical map of the Bacillus subtilis 168 chromosome constructed by a gene directed mutagenesis method. J. Mol. Biol, 220, 631-648.
14. Gibson, D.G., Young, L., Chuang, R.Y., Venter, J.C., Hutchison, C.A. III and Smith, H.O. (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods, 6, 343-345.
15. Tsuge, K., Matsui, K. and Itaya, M. (2007) Production of the non-ribosomal peptide plipastatin in Bacillus subtilis regulated by three relevant gene blocks assembled in a single movable DNA segment. J. Biotech., 129, 592-603.
16. Itaya, M. and Crouch, R.J. (1991) Correlation of activity with phenotypes of Escherichia coli partial function mutant of rnh, the gene encoding RNase H. Mol. Gen. Genet., 227, 433-437.
17. Itaya, M. and Tanaka, T. (1997) Predicted and unsuspected alterations of the genome structure of genetically defined Bacillus subtilis 168 strains. Biosci. Biotechnol. Biochem., 61, 56-64.
18. Ohashi, Y., Ohshima, H., Tsuge, K. and Itaya, M. (2003) Far different levels of gene expression provided by an oriented cloning system in Bacillus subtilis and Escherichia coli. FEMS Microbiol. Lett., 221, 125-130.