Tracking chondrocytes and assessing their proliferation with carboxyfluorescein diacetate succinimidyl ester: Effects on cell functions

Tissue Engineering - Part C: Methods

Volumn 16, Issue 2, 2010, Pages 301-310

  Chawla, Kanika ^a,b   Masuda, Koichi ^c,d   Sah, Robert L ^a,e  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d RUSH UNIVERSITY MEDICAL CENTER   (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BODY FLUIDS; CELL PROLIFERATION; ESTERIFICATION; ESTERS; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; MONOLAYERS; SUSPENSIONS (FLUIDS); SYNTHESIS (CHEMICAL);

CARBOXYFLUORESCEIN; CELL FUNCTIONS; CELL LABELING; CELL SUSPENSION; CELL-BASED; CHONDROCYTE PROLIFERATION; CHONDROCYTES; GLYCOSAMINOGLYCANS; HIGH-DENSITY; IN-VIVO; PROTEOGLYCANS; SUCCINIMIDYL ESTER;

CELL CULTURE;

5 (6) CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; 5-(6)-CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; PROTEOGLYCAN; SUCCINIMIDE DERIVATIVE;

ANIMAL; ARTICLE; BIOLOGICAL MODEL; CARTILAGE CELL; CATTLE; CELL COUNT; CELL CULTURE; CELL MOTION; CELL PROLIFERATION; CYTOLOGY; DRUG EFFECT; EVALUATION; METABOLISM; METHODOLOGY; MONITORING; PHYSIOLOGY; STAINING;

ANIMALS; CATTLE; CELL COUNT; CELL MOVEMENT; CELL PROLIFERATION; CELLS, CULTURED; CHONDROCYTES; FLUORESCEINS; FLUORESCENT DYES; MODELS, BIOLOGICAL; MONITORING, PHYSIOLOGIC; PROTEOGLYCANS; STAINING AND LABELING; SUCCINIMIDES;

EID: 77952380155     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2008.0609     Document Type: Article

References (44)

1. Sah, R.L., Klein, T.J., Schmidt, T.A., Albrecht, D.R., Bae, W.C., Nugent, G.E., McGowan, K.B., Temple, M.M., Jadin, K.D., Schumacher, B.L., Chen, A.C., and Sandy, J.D. Articular cartilage repair, regeneration, and replacement. In: Koopman, W.J., ed. Arthritis and Allied Conditions: A Textbook of Rheumatology. Philadelphia, PA: Lippincott Williams & Wilkins, 2005, pp. 2277-2301.
2. Baragi, V.M., Renkiewicz, R.R., Qiu, L., Brammer, D., Riley, J.M., Sigler, R.E., Frenkel, S.R., Amin, A., Abramson, S.B., and Roessler, B.J. Transplantation of adenovirally transduced allogeneic chondrocytes into articular cartilage defects in vivo. Osteoarthritis Cartilage 5, 275, 1997.
3. Brittberg, M. Autologous chondrocyte transplantation. Clin Orthop Relat Res 367S, 147, 1999.
4. Hendrickson, D.A., Nixon, A.J., Grande, D.A., Todhunter, R.J., Minor, R.M., Erb, H., and Lust, G. Chondrocyte-fibrin matrix transplants for resurfacing extensive articular cartilage defects. J Orthop Res 12, 485, 1994.
5. Elisseeff, J., Anseth, K., Sims, D., McIntosh, W., Randolph, M., and Langer, R. Transdermal photopolymerization for minimally invasive implantation. Proc Natl Acad Sci USA 96, 3104, 1999.
6. Schreiber, R.E., Dunkelman, N.S., Naughton, G., and Rat-cliffe, A. A method for tissue engineering of cartilage by cell seeding on bioresorbable scaffolds. Ann NY Acad Sci 875, 398, 1999.
7. Marlovits, S., Striessnig, G., Kutscha-Lissberg, F., Resinger, C., Aldrian, S.M., Vecsei, V., and Trattnig, S. Early postoperative adherence of matrix-induced autologous chon-drocyte implantation for the treatment of full-thickness cartilage defects of the femoral condyle. Knee Surg Sports Traumatol Arthrosc 13, 451, 2005.
8. Green, W.T. Articular cartilage repair: behavior of rabbit chondrocytes during tissue culture and subsequent allo-grafting. Clin Orthop Relat Res 124, 237, 1977.
9. Billings, E., von Schroeder, H.P., Mai, M.T., Aratow, M., Amiel, D., Woo, S.L.-Y., and Coutts, R.D. Cartilage resurfacing of the rabbit knee: the use of an allogeneic deminer-alized bone matrix-autogeneic perichondrium composite implant. Acta Orthop Scand 61, 201, 1990.
10. Ostrander, R.V., Goomer, R.S., Tontz, W.L., Khatod, M., Harwood, F.L., Maris, T.M., and Amiel, D. Donor cell fate in tissue engineering of articular cartilage repair. Clin Orthop Relat Res 389, 228, 2001.
11. Haugland, R.P. Molecular Probes Handbook of Fluorescent Probes and Research Chemicals. Eugene, OR: Molecular Probes, Inc., 1996.
12. Horan, P.K., and Slezak, S.E. In vivo cellular tracking. United States Patent #4,762,701, 1986.
13. Ashley, D.M., Bol, S.J., and Kannourakis, G. Measurement of the growth parameters of precursor B-acute lymphoblastic leukaemic cells in co-culture with bone marrow stromal cells; detection of two cd10 positive populations with different proliferative capacities and survival. Leuk Res 18, 37, 1994.
14. Ashley, D.M., Bol, S.J., Waugh, C., and Kannourakis, G. A novel approach to the measurement of different in vitro leukaemic cell growth parameters: the use of PKH GL fluorescent probes. Leuk Res 17, 873, 1993.
15. Boutonnat, J., Barbier, M., Rousselle, C., Muirhead, K.A., Mousseau, M., Seigneurin, D., and Ronot, X. Usefulness of PKHs for studying cell proliferation. C R Acad Sci III 321, 901, 1998.
16. Chawla, K., Klein, T.J., Schumacher, B.L., Schmidt, T.A., Voegtline, M.S., Thonar, E.J.-M.A., Masuda, K., and Sah, R.L. Tracking chondrocytes and assessing their proliferation with PKH26: effects on secretion of proteoglycan 4 (PRG4). J Orthop Res 24, 1499, 2006.
17. Yamamura, Y., Rodriguez, N., Schwartz, A., Eylar, E., Bagwell, B., and Yano, N. A new flow cytometric method for quantitative assessment of lymphocyte mitogenic potentials. Cell Mol Biol 41 Suppl 1, S121, 1995.
18. Fox, D., Kouris, G.J., Blumofe, K.A., Heilizer, T.J., Husak, V., and Greisler, H.P. Optimizing fluorescent labeling of endo-thelial cells for tracking during long-term studies of autol-ogous transplantation. J Surg Res 86, 9, 1999.
19. Samlowski, W.E., Robertson, B.A., Draper, B.K., Prystas, E., and McGregor, J.R. Effects of supravital fluorochromes used to analyze the in vivo homing of murine lymphocytes on cellular function. J Immunol Methods 144, 101, 1991.
20. DellAccio, F., Vanlauwe, J., Bellemans, J., News, J., DeBari, C., and Luyten, F.P. Expanded phenotypically stable chon-drocytes persist in the repair tissue and contribute to cartilage matrix formation and structural integration in a goat model of autologous chondrocyte implantation. J Orthop Res 21, 123, 2003.
21. Chawla, K., Klein, T.J., Schumacher, B.L., Jadin, K.D., Shah, B.H., Nakagawa, K., Wong, V.W., Chen, A.C., Masuda, K., and Sah, R.L. Short-term retention of chondrocytes in stratified tissue-engineered cartilaginous constructs implanted in vivo in mini-pigs. Tissue Eng 13, 1525, 2007.
22. Parish, C.R. Fluorescent dyes for lymphocyte migration and proliferation studies. Immunol Cell Biol 77, 499, 1999.
23. Gruber, H.E., Leslie, K.P., Ingram, J.A., and Hanley, E.N., Jr. Optimization of 5-(and-6)-carboxyfluorescein diacetate suc-cinimidyl ester for labeling human intervertebral disc cells in vitro. Biotech Histochem 75, 118, 2000.
24. Asquith, B., Debacq, C., Florins, A., Gillet, N., Sanchez-Alcaraz, T., Mosley, A., and Willems, L. Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (CFSE). Proc Biol Sci 273, 1165, 2006.
25. Li, X., Dancausse, H., Grijalva, I., Oliveira, M., and Levi, A.D. Labeling Schwann cells with CFSE\an in vitro and in vivo study. J Neurosci Methods 125, 83, 2003.
26. Urbani, S., Caporale, R., Lombardini, L., Bosi, A., and Sac-cardi, R. Use of CFDA-SE for evaluating the in vitro proliferation pattern of human mesenchymal stem cells. Cytotherapy 8, 243, 2006.
27. Wang, X.Q., Duan, X.M., Liu, L.H., Fang, Y.Q., and Tan, Y. Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling. Acta Biochim Biophys Sin (Shanghai) 37, 379, 2005.
28. Jay, G.D., Tantravahi, U., Britt, D.E., Barrach, H.J., and Cha, C.J. Homology of lubricin and superficial zone protein (SZP): products of megakaryocyte stimulating factor (MSF) gene expression by human synovial fibroblasts and articular chondro-cytes localizedto chromosome 1q25. J Orthop Res 19, 677, 2001.
29. Shapiro, H.M. Practical Flow Cytometry. Hoboken, NJ: Wiley-Liss, 2003.
30. Klein, T.J., Schumacher, B.L., Schmidt, T.A., Li, K.W., Voegtline, M.S., Masuda, K., Thonar, E.J.-M.A., and Sah, R.L. Tissue engineering of stratified articular cartilage from chon-drocyte subpopulations. Osteoarthritis Cartilage 11, 595, 2003.
31. Kim, Y.J., Sah, R.L.Y., Doong, J.Y.H., and Grodzinsky, A.J. Fluorometric assay of DNA in cartilage explants using Hoechst 33258. Anal Biochem 174, 168, 1988.
32. McGowan, K.B., Kurtis, M.S., Lottman, L.M., Watson, D., and Sah, R.L. Biochemical quantification of DNA in human articular and septal cartilage using PicoGreen and Hoechst 33258. Osteoarthritis Cartilage 10, 580, 2002.
33. Farndale, R.W., Buttle, D.J., and Barrett, A.J. Improved quantitation and discrimination of sulphated glycosamino-glycans by use of dimethylmethylene blue. Biochim Biophys Acta 883, 173, 1986.
34. Schumacher, B.L., Block, J.A., Schmid, T.M., Aydelotte, M.B., and Kuettner, K.E. A novel proteoglycan synthesized and secreted by chondrocytes of the superficial zone of articular cartilage. Arch Biochem Biophys 311, 144, 1994.
35. Bronner-Fraser, M. Alterations in neural crest migration by a monoclonal antibody that affects cell adhesion. J Cell Biol 101, 610, 1985.
36. Murphy, S.J., Watt, D.J., and Jones, G.E. An evaluation of cell separation techniques in a model mixed cell population. J Cell Sci 102 (Pt 4), 789, 1992.
37. Dooner, M., Cerny, J., Colvin, G., Demers, D., Pimentel, J., Greer, D., Abedi, M., McAuliffe, C., and Quesenberry, P. Homing and conversion of murine hematopoietic stem cells to lung. Blood Cells Mol Dis 32, 47, 2004.
38. Sukkar, M.B., Stanley, A.J., Blake, A.E., Hodgkin, P.D., Johnson, P.R., Armour, C.L., and Hughes, J.M. "Proliferative" and "synthetic" airway smooth muscle cells are overlapping populations. Immunol Cell Biol 82, 471, 2004.
39. Walker, B., Gray, J., Burns, D.M., Wang, Q., Adrian, T.E., Nichols, D.H., Murphy, R.F., and Nelson, J. Carboxy-fluorescein and biotin neuromedin C analogues: synthesis and applications. Peptides 16, 255, 1995.
40. Lyons, A.B., and Parish, C.R. Determination of lymphocyte division by flow cytometry. J Immunol Methods 171, 131, 1994.
41. Li, K.W., Falcovitz, Y.H., Nagrampa, J.P., Chen, A.C., Lott-man, L.M., Shyy, Y.J., and Sah, R.L. Mechanical compression modulates proliferation of transplanted chondrocytes. J Or-thop Res 18, 374, 2000.
42. Schmidt, T.A., Schumacher, B.L., Klein, T.J., Voegtline, M.S., and Sah, R.L. Synthesis of proteoglycan 4 by chondrocyte subpopulations in cartilage explants, monolayer cultures, and resurfaced cartilage cultures. Arthritis Rheum 50, 2849, 2004.
43. Chawla, K., Klein, T.J., Schumacher, B.L., Jadin, K.D., Schmidt, T.A., Chen, A.C., Wong, V.L., Nakagawa, K., Thonar, E.J.-M.A., Masuda, K., and Sah, R.L. Enhanced formation of stratified tissue-engineered porcine cartilage from chondrocyte subpopulations with bone morphogenetic protein-7 (BMP-7). Trans Orthop Res Soc 30, 294, 2005.
44. Masuda, K., Pfister, B.E., Sah, R.L., and Thonar, E.J.-M.A. Osteogenic protein-1 promotes the formation of tissue-engineered cartilage using the alginate-recovered-chondrocyte method. Osteoarthritis Cartilage 14, 384, 2006.