Effects of spontaneous mutations in PipX functions and regulatory complexes on the cyanobacterium Synechococcus elongatus strain PCC 7942

Microbiology

Volumn 156, Issue 5, 2010, Pages 1517-1526

  Espinosa, Javier ^a   Castells, Miguel Angel ^a   Laichoubi, Karim Boumediene ^a   Forchhammer, Karl ^b   Contreras, Asunción ^a  

^a UNIVERSITY OF ALICANTE   (Spain)

^b UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

N ACETYLGLUTAMIC ACID;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CONTROLLED STUDY; CYANOBACTERIUM; GENE FUNCTION; GENE MUTATION; GLNB GENE; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PIX GENE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; REGULATORY MECHANISM; SUPPRESSOR GENE; SYNECHOCOCCUS ELONGATUS; WILD TYPE;

BACTERIAL PROTEINS; CARBON; GLUTAMIC ACID; NITROGEN; PII NITROGEN REGULATORY PROTEINS; POINT MUTATION; SUPPRESSION, GENETIC; SYNECHOCOCCUS;

CYANOBACTERIA; SYNECHOCOCCUS ELONGATUS; SYNECHOCOCCUS ELONGATUS PCC 6301;

EID: 77952371434     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.037309-0     Document Type: Article

References (39)

1. Aldehni, M. F. & Forchhammer, K. (2006). Analysis of a non-canonical NtcA-dependent promoter in Synechococcus elongatus and its regulation by NtcA and PII. Arch Microbiol 184, 378-386.
2. II is required for NtcA-regulated gene expression during nitrogen deprivation in the cyanobacterium Synechococcus elongatus strain PCC 7942. J Bacteriol 185, 2582-2591.
3. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors) (1999). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology. New York, NY: Wiley.
4. Bartel, P., Chien, C. T., Sternglanz, R. & Fields, S. (1993). Elimination of false-positives that arise in using the two-hybrid system. Biotechniques 14, 920-924. (Pubitemid 23166805)
5. Burillo, S., Luque, I., Fuentes, I. & Contreras, A. (2004). Interactions between the nitrogen signal transduction protein PII and N-acetyl glutamate kinase in organisms that perform oxygenic photosynthesis. J Bacteriol 186, 3346-3354. (Pubitemid 38661529)
6. Chen, Y. M., Ferrar, T. S., Lohmeir-Vogel, E., Morrice, N., Mizuno, Y., Berenger, B., Ng, K. K., Muench, D. G. & Moorhead, G. B. (2006). The PII signal transduction protein of Arabidopsis thaliana forms an arginine-regulated complex with plastid N-acetyl glutamate kinase. J Biol Chem 281, 5726-5733. (Pubitemid 43847671)
7. Crooks, G. E., Hon, G., Chandonia, J. M. & Brenner, S. E. (2004). WebLogo: a sequence logo generator. Genome Res 14, 1188-1190.
8. Elhai, J. & Wolk, C. P. (1988). A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68, 119-138.
9. Espinosa, J., Forchhammer, K., Burillo, S. & Contreras, A. (2006). Interaction network in cyanobacterial nitrogen regulation: PipX, a protein that interacts in a 2-oxoglutarate dependent manner with PII and NtcA. Mol Microbiol 61, 457-469. (Pubitemid 44356576)
10. Espinosa, J., Forchhammer, K. & Contreras, A. (2007). Role of the Synechococcus PCC 7942 nitrogen regulator protein PipX in NtcA-controlled processes. Microbiology 153, 711-718. (Pubitemid 46444369)
11. Espinosa, J., Castells, M. A., Forchhammer, K. & Contreras, A. (2008). Regulatory interactions involved in nitrogen signalling in cyanobacteria: partner swapping and role of PipX-PII complexes in Synechococcus sp. PCC 7942. In Biological Nitrogen Fixation: Towards Poverty Alleviation through Sustainable Agriculture, pp. 361-362. Edited by F. D. Dakora, S. B. M. Chimphango, A. J. Valentine, C. Elmerich & W. E. Newton. Dordrect, The Netherlands: Springer.
12. Espinosa, J., Castells, M. A., Laichoubi, K. B. & Contreras, A. (2009). Mutations at pipX suppress lethality of PII-deficient mutants of Synechococcus elongatus PCC 7942. J Bacteriol 191, 4863-4869.
13. II signal transduction in cyanobacteria: from signals to targets. FEMS Microbiol Rev 28, 319-333.
14. II signal transducers: novel functional and structural insights. Trends Microbiol 16, 65-72.
15. Golden, S. S. & Sherman, L. A. (1984). Optimal conditions for genetic transformation of the cyanobacterium Anacystis nidulans R2. J Bacteriol 158, 36-42.
16. Hanahan, D. (1985). Techniques for transformation of Escherichia coli. In DNA Cloning, pp. 109-135. Edited by D. M. Glover. Oxford, UK: IRL Press.
17. Harper, J. W., Adami, G. R., Wei, N., Keyomarsi, K. & Elledge, S. J. (1993). The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75, 805-816. (Pubitemid 23346377)
18. Herrero, A., Muro-Pastor, A. M. & Flores, E. (2001). Nitrogen control in cyanobacteria. J Bacteriol 183, 411-425.
19. Hisbergues, M., Jeanjean, R., Joset, F., Tandeau de Marsac, N. & Bedu, S. (1999). Protein PII regulates both inorganic carbon and nitrate uptake and is modified by a redox signal in Synechocystis PCC 6803. FEBS Lett 463, 216-220. (Pubitemid 30001922)
20. James, P., Halladay, J. & Craig, E. A. (1996). Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics 144, 1425-1436. (Pubitemid 26427892)
21. II phosphatase PphA is required for light-dependent control of nitrate utilization in Synechocystis sp. strain PCC 6803. J Bacteriol 187, 6683-6690.
22. Kolonin, M. G., Zhong, J. & Finley, R. L. (2000). Interaction mating methods in two-hybrid systems. Methods Enzymol 328, 26-46.
23. Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A. & other authors (2007). CLUSTAL W and CLUSTAL-X version 2.0. Bioinformatics 23, 2947-2948.
24. Laurent, S., Chen, H., Bedu, S., Ziarelli, F., Peng, L. & Zhang, C. C. (2005). Nonmetabolizable analogue of 2-oxoglutarate elicits heterocyst differentiation under repressive conditions in Anabaena sp. PCC 7120. Proc Natl Acad Sci U S A 102, 9907-9912. (Pubitemid 40993666)
25. II-mediated regulation of nitrate and nitrite uptake in the Cyanobacterium synechococcus sp. PCC 7942. Eur J Biochem 267, 591-600.
26. Leigh, J. A. & Dodsworth, J. A. (2007). Nitrogen regulation in bacteria and archaea. Annu Rev Microbiol 61, 349-377.
27. II controls the storage of nitrogen as arginine. Proc Natl Acad Sci U S A 104, 17644-17649.
28. Luque, I., Zabulon, G., Contreras, A. & Houmard, J. (2001). Convergence of two global transcriptional regulators on nitrogen induction of the stress-acclimation gene nblA in the cyanobacterium Synechococcus sp. PCC 7942. Mol Microbiol 41, 937-947. (Pubitemid 34209001)
29. Maheswaran, M., Urbanke, C. & Forchhammer, K. (2004). Complex formation and catalytic activation by the PII signaling protein of N-acetyl-L-glutamate kinase from Synechococcus elongatus strain PCC 7942. J Biol Chem 279, 55202-55210.
30. Muro-Pastor, M. I., Reyes, J. C. & Florencio, F. J. (2001). Cyanobacteria perceive nitrogen status by sensing intracellular 2- oxoglutarate levels. J Biol Chem 276, 38320-38328.
31. Muro-Pastor, M. I., Reyes, J. C. & Florencio, F. J. (2005). Ammonium assimilation in cyanobacteria. Photosynth Res 83, 135-150.
32. Osanai, T., Sato, S., Tabata, S. & Tanaka, K. (2005). Identification of PamA as a PII-binding membrane protein important in nitrogen-related and sugar-catabolic gene expression in Synechocystis sp. PCC 6803. J Biol Chem 280, 34684-34690. (Pubitemid 41504600)
33. Ruiz, D., Salinas, P., Lopes-Redondo, M. L., Cayuela, M. L., Marina, A. & Contreras, A. (2008). Phosphorylation-independent activation of the atypical response regulator NblR. Microbiology 154, 3002-3015.
34. Salinas, P., Ruiz, D., Cantos, R., Lopez-Redondo, M. L., Marina, A. & Contreras, A. (2007). The regulatory factor SipA provides a link between NblS and NblR signal transduction pathways in the cyanobacterium Synechococcus sp. PCC 7942. Mol Microbiol 66, 1607-1619. (Pubitemid 350179692)
35. Sauer, J., Dirmeier, U. & Forchhammer, K. (2000). The Synechococcus strain PCC 7942 glnN product (glutamine synthetase III) helps recovery from prolonged nitrogen chlorosis. J Bacteriol 182, 5615-5619.
36. Sugiyama, K., Hayakawa, T., Kudo, T., Ito, T. & Yamaya, T. (2004). Interaction of N-acetylglutamate kinase with a PII-like protein in rice. Plant Cell Physiol 45, 1768-1778.
37. Takatani, N., Kobayashi, M., Maeda, S. & Omata, T. (2006). Regulation of nitrate reductase by non-modifiable derivatives of PII in the cells of Synechococcus elongatus strain PCC 7942. Plant Cell Physiol 47, 1182-1186. (Pubitemid 44453560)
38. Tanigawa, R., Shirokane, M., Maeda Si, S., Omata, T., Tanaka, K. & Takahashi, H. (2002). Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro. Proc Natl Acad Sci U S A 99, 4251-4255.
39. Vazquez-Bermudez, M. F., Herrero, A. & Flores, E. (2002). 2-Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoter. FEBS Lett 512, 71-74. (Pubitemid 34164453)