The use of affinity adsorbents in expanded bed adsorption

Journal of Molecular Recognition

Volumn 11, Issue 1-6, 1998, Pages 217-221

  Chase, Howard Allaker ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Affinity separations; Cell separations; Expanded bed adsorption; Ligands; Nucleic acids; Purification

Indexed keywords

EXPANDED BED ADSORPTION; NUCLEIC ACID; PROTEIN; PURIFICATION; SEPARATION TECHNIQUE;

EID: 0032456781     PISSN: 09523499     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1099-1352(199812)11:1/6<217::AID-JMR426>3.0.CO;2-D     Document Type: Conference Paper

References (19)

1. Arnold, F. H. (1991). Metal-affinity separations - a new dimension in protein processing. Bio-Technology 9, 151-156.
2. Bjorklund, M. and Hearn, M. T. W. (1996). Characterization of silica-based heparin-affinity adsorbents through column chromatography of plasma fractions containing thrombin. J. Chromatogr. A 743, 145-162.
3. Brena, B. M., Ryden, L. G. and Porath, J. (1994). Immobilization of beta-galactosidase on metal-chelate-substituted gels. Biotechnol. Appl. Biochem. 19, 217-231.
4. Caflisch, A. (1996). Computational combinatorial ligand design-application to human alpha-thrombin. J. Comput.-Aided Mol. Design 10, 372-396.
5. Chang, Y. K. and Chase, H. A. (1996a). Ion-exchange purification of G6PDH from unclarified yeast-cell homogenates using expanded bed adsorption. Biotechnol. Bioengng. 49, 204-216.
6. Chang, Y. K. and Chase, H. A. (1996b). Development of operating-conditions for protein-purification using expanded bed techniques - the effect of the degree of bed expansion on adsorption performance. Biotechnol. and Bioengng. 49, 512-526.
7. Chang, Y. K., McCreath, G. E. and Chase, H. A. (1995). Development of an expanded bed technique for an affinity purification of G6PDH from unclarified yeast-cell homogenates. Biotechnol. Bioengng. 48, 355-366.
8. Chase, H. A. (1994). Purification of proteins by adsorption chromatography in expanded beds. Trends Biotechnol. 12, 296-303.
9. Chase, H. A. and Draeger, N. M. (1992). Affinity purification of proteins using expanded beds. J. Chromatogr. 597, 129-145.
10. Clackson, T. and Wells, J. A. (1994). In-vitro selection from protein and peptide libraries. Trends Biotechnol. 12, 173-184.
11. Draeger, N. M. and Chase, H. A. (1991). Liquid fluidized-bed adsorption of protein in the presence of cells. Bioseparation 2, 67-80.
12. Ellingsen, T., Aune, O., Berge, A., Kilaas, L., Schmid, R., Stenstad, P., Ugelstad, J., Hagen, S., Weng, E. and Johansen, L. (1993). Monosized polymer particles in biochemical and biomedical separations. Makromol. Chem.-Macromol. Symp. 70, 315-326.
13. Fassina, G., Verdoliva, A., Odierna, M. R., Ruvo, M. and Cassini, G. (1996). Protein A mimetic peptide ligand for affinity purification of antibodies. J. Mol. Recognit. 9, 564-569.
14. Fluit, A. C., Widjojoatmodjo, M. N. and Verhoef, J. (1995). Detection of Salmonella species in fecal samples by immunomagnetic separation and PCR. J. Clin. Microbiol. 33, 1046-1046.
15. Frej, A. K. B., Hjorth, R. and Hammarstrom, A. (1994). Pilot-scale recovery of recombinant annexin-V from unclarified Escherichia-coli homogenate using expanded bed adsorption, Biotechnol. Bioengng. 44, 922-929.
16. Kvalheim, G., Pharo, A., Fodstad, O., Holte, H., Erikstein, B., Nesland, L., Smeland, E. and Wang, M. Y. (1996). Purging of breast-cancer cells from leukapheresis products. Blood 88, 994-994.
17. McCreath, G. E. and Chase, H. A. (1996a). Affinity adsorption of Saccharomyces cerevisiae on concanavalin A perfluorocarbon emulsions. J. Mol. Recognit. 9, 607-616.
18. McCreath, G. E. and Chase, H. A. (1996b). Applications of perfluorocarbon affinity emulsions for the rapid isolation of Staphylococcus-aureus. Biotechnol. Prog. 12, 77-83.
19. McCreath, G. E., Chase, H. A., Owen, R. O. and Lowe, C. R. (1995). Expanded bed affinity-chromatography of dehydrogenases from bakers-yeast using dye-ligand perfluoropolymer supports. Biotechnol. Bioengng. 48, 341-354.