Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column

Biochemical Engineering Journal

Volumn 50, Issue 3, 2010, Pages 90-98

  Luechau, Frank ^a   Ling, Tau Chuan ^a   Lyddiatt, Andrew ^a,b  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b NEWCASTLE UNIVERSITY BUSINESS SCHOOL   (United Kingdom)

Author keywords

Aqueous two phase system; Bioseparations; Extraction; Physical; Precipitation

Indexed keywords

AQUEOUS TWO PHASE SYSTEM; AQUEOUS TWO-PHASE EXTRACTION; AXIAL DISPERSION COEFFICIENTS; BIO SEPARATION; BIOSEPARATIONS; DISPERSED PHASE HOLD-UP; HOLD UP; HYDROGEN PHOSPHATES; IMPELLER SPEED; MAIN PARAMETERS; MOBILE PHASE; MOBILE PHASE FLOW RATE; PLASMID DNA (PDNA); SINGLE STAGE; STATIONARY PHASE;

HYDROGEN; PHASE MODULATION; POLYETHYLENE GLYCOLS; POTASSIUM; SOLVENT EXTRACTION;

PHASE SEPARATION;

DIPOTASSIUM HYDROGEN PHOSPHATE; MACROGOL; MACROGOL 1450; PLASMID DNA; UNCLASSIFIED DRUG;

ANALYTICAL PARAMETERS; ARTICLE; CHROMATOGRAPHY; DENSITY; DISPERSION; FLOW RATE; LIQUID LIQUID EXTRACTION; MOBILE PHASE; PH; PHASE SEPARATION; PRIORITY JOURNAL; SINGLE STAGE KUHNI TYPE AQUEOUS TWO PHASE EXTRACTION; VISCOSITY;

EID: 77952321097     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2010.02.010     Document Type: Article

References (36)

1. Rito-Palomares M. Practical application of aqueous two-phase partition to process development for the recovery of biological products. J. Chromatogr. B 807 (2004) 3-11
2. Naganagouda K., and Mulimani V.H. Aqueous two-phase extraction (ATPE): an attractive and economically viable technology for downstream processing of Aspergillus oryzae α-galactosidace. Process Biochem. 43 (2008) 1293-1299
3. Azevedo A.M., Rosa P.A.J., Ferreira I.F., and Aires-Barros M.R. Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing. Trends Biotechnol. 27 (2009) 240-247
4. Przybycien T.M., Pujar N.S., and Steele L.M. Alternative bioseparation operations: life beyond packed-bed chromatography. Curr. Opin. Biotechnol. 15 (2004) 469-478
5. Johansson H.-O., Magaldi F.M., Feitosa E., and Pessoa Jr. A. Protein partitioning in poly(ethylene glycol)/sodium polyacrylate aqueous two-phase systems. J. Chromatogr. A 1178 (2008) 145-153
6. Ito Y., and Bowman R.L. Application of the elution centrifuge to separation of polynucleotides with the use of polymer phase systems. Science 182 (1973) 391-393
7. Shibusawa Y., Matsuda K., and Ito Y. Countercurrent chromatography of proteins with polymer phase systems. In: Desai M.A. (Ed). Downstream Processing of Protein, Methods and Protocols (2000), Humana Press Inc., New Jersey 161-172
8. Perry R.H., and Green D.W. Perry's Chemical Engineers' Handbook. 7th ed. (1997), McGraw Hill, New York
9. Hustedt H., Kroner K.H., Stach W., and Kula M.R. Procedure for the simultaneous large-scale isolation of pullulanase and 1,4-alpha-glucan phosporylase from Klebsiella pneumoniae involving liquid-liquid separations. Biotechnol. Bioeng. 20 (1978) 1989-2005
10. Kula M.R., Kroner K.H., and Hustedt H. Purification of enzymes by liquid-liquid extraction. Adv. Biochem. Eng. 24 (1982) 73-118
11. Kroner K.H., and Hustedt H. Gravity settling of APS-characteristics and process applications. In: Fisher D., and Sutherland I.A. (Eds). Separations Using Aqueous Phase Systems (1989), Plenum Press, New York 337-339
12. Salamanca M.H., Merchuk J.C., Andrews B.A., and Asenjo J.A. On the kinetics of phase separation in aqueous two-phase systems. J. Chromatogr. B 711 (1998) 319-329
13. Albertsson P.A. Partition of Cell Particles and Macromolecules (1986), Wiley, New York
14. Blomquist G., and Albertsson P.A. A study of extraction columns for aqueous polymer two-phase systems. J. Chromatogr. 73 (1972) 125-133
15. Treybal R.E. Mass Transfer Operations (1980), McGraw-Hill, New York
16. Diamond A.D., and Hsu J.T. Aqueous two-phase systems for biomolecule separation. Adv. Biochem. Eng. 47 (1992) 89-135
17. Raghavarao K.S.M.S., Rastogi N.K., Gowthaman M.K., and Karanth N.G. Aqueous two-phase extraction for downstream processing of enzymes/proteins. Adv. Appl. Microbiol. 41 (1995) 97-171
18. Save S.S., and Save S.V. Mass transfer in aqueous two-phase systems. In: Rogers R.D., and Eiteman M.A. (Eds). Aqueous Biphasic Separations, Biomolecules to Metal Ions (1995), Plenum Press, New York 71-81
19. Blomquist G., and Albertsson P.A. An extraction unit for analyzing nucleic acids and other macromolecules. Anal. Biochem. 30 (1969) 222-229
20. Shanbhag V.P. Diffusion of proteins across a liquid-liquid interface. Biochim. Biophys. Acta 320 (1973) 517-527
21. Patil T.A., Sawant S.B., and Joshi J.B. Mass transfer coefficients in two-phase aqueous extraction. Chem. Eng. J. 39 (1988) 1-6
22. Levenspiel O. Chemical Reaction Engineering (1972), Wiley, New York
23. APB (Amersham Pharmacia Biotech). Expanded Bed Adsorption-Principles and Methods (1998) Uppsala, Sweden
24. Luechau F., Ling T.C., and Lyddiatt A. Partition of plasmid DNA in polymer-salt aqueous two phase systems. Sep. Purif. Technol. 66 (2009) 397-404
25. Luechau F., Ling T.C., and Lyddiatt A. Primary capture of high molecular weight nucleic acids using aqueous two-phase systems. Sep. Purif. Technol. 66 (2009) 202-207
26. Barnea E., and Mizrahi J. Separation mechanism of liquid-liquid dispersions in a deep-layer gravity settler: Part I-The structure of the dispersion band. Trans. Inst. Chem. Eng. 53 (1975) 61-69
27. Barnea E., and Mizrahi J. Separation mechanism of liquid-liquid dispersions in a deep-layer gravity settler: Part II-Flow patterns of the dispersed and continuous phases within the dispersion band. Trans. Inst. Chem. Eng. 53 (1975) 70-74
28. Barnea E., and Mizrahi J. Separation mechanism of liquid-liquid dispersions in a deep-layer gravity settler: Part III-Hindered settling and drop-to-drop coalescence in the dispersion band. Trans. Inst. Chem. Eng. 53 (1975) 75-82
29. Lo T.C. Commercial liquid-liquid extraction equipment. In: Schweiter P.A. (Ed). Handbook of Separation Techniques for Chemical Engineers. 2nd ed. (1988), McGraw Hill, New York
30. Coimbra J.D.R., Thoemmes J., and Kula M.R. Continuous separation of whey proteins with aqueous two-phase systems in a Graesser contactor. J. Chromatogr. A 668 (1994) 85-94
31. Coimbra J.D.R., Thoemmes J., Meirelles A.J., and Kula M.R. Performance of a Graesser contactor in the continuous extraction of whey proteins: mixing, mass transfer and efficiency. Bioseparation 5 (1995) 259-268
32. Patil T.A., Jafarabad K.R., Sawant S.B., and Joshi J.B. Enzyme mass transfer coefficient in aqueous two phase system using a packed extraction column. Can. J. Chem. Eng. 69 (1991) 548-556
33. Jafarabad K.R., Sawant S.B., and Joshi J.B. Enzyme and protein mass transfer coefficient in aqueous two-phase systems-I, Spray extraction columns. Chem. Eng. Sci. 37 (1992) 57-68
34. Jafarabad K.R., Patil T.A., Sawant S.B., and Joshi J.B. Enzyme and protein mass transfer coefficient in aqueous two-phase systems-II, York-Scheibel extraction column. Chem. Eng. Sci. 37 (1992) 69-73
35. Sawant S.B., and Sikdar S.K. Hydrodynamics and mass transfer in two-phase aqueous extraction using spray columns. Biotechnol. Bioeng. 36 (1990) 109-115
36. Joshi J.B., Sawant S.B., Patil T.A., Jafarabad K.R., Raghavarao K.S.M.S., and Sikdar S.K. Continuous counter-current two-phase aqueous extraction. Bioseparation 1 (1990) 311-324