Crystal structure of the leucine aminopeptidase from pseudomonas putida reveals the molecular basis for its enantioselectivity and broad substrate specificity

Journal of Molecular Biology

Volumn 398, Issue 5, 2010, Pages 703-714

  Kale, Avinash ^a   Pijning, Tjaard ^a   Sonke, Theo ^b   Dijkstra, Bauke W ^a   Thunnissen, Andy Mark W H ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b DSM Pharmaceutical Products   (Netherlands)

Author keywords

Di zinc proteases; Enantioselectivity; Leucine aminopeptidase; Substrate specificity; X ray crystallography

Indexed keywords

BESTATIN; CYTOSOL AMINOPEPTIDASE; LYSINE; MANGANESE; ZINC ION; BACTERIAL PROTEIN; DIVALENT CATION; DRUG DERIVATIVE; ENZYME ACTIVATOR; ENZYME INHIBITOR; LEUCINE; ZINC;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENANTIOSELECTIVITY; ENZYME ACTIVE SITE; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; MOLECULAR BIOLOGY; MOLECULAR MODEL; NONHUMAN; PH MEASUREMENT; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTON TRANSPORT; PSEUDOMONAS PUTIDA; X RAY CRYSTALLOGRAPHY; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; METABOLISM; PH; PROTEIN BINDING; PROTEIN QUATERNARY STRUCTURE; STEREOISOMERISM;

BOVINAE; ESCHERICHIA COLI; PSEUDOMONAS PUTIDA;

BACTERIAL PROTEINS; CATALYTIC DOMAIN; CATIONS, DIVALENT; CRYSTALLOGRAPHY, X-RAY; ENZYME ACTIVATORS; ENZYME INHIBITORS; HYDROGEN-ION CONCENTRATION; LEUCINE; LEUCYL AMINOPEPTIDASE; MANGANESE; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN STRUCTURE, QUATERNARY; PSEUDOMONAS PUTIDA; STEREOISOMERISM; SUBSTRATE SPECIFICITY; ZINC;

EID: 77952295492     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2010.03.042     Document Type: Article

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