Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato

European Journal of Biochemistry

Volumn 269, Issue 6, 2002, Pages 1630-1640

  Gu, Yong Qiang ^a,b   Walling, Linda L ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b WESTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

Aminopeptidases; Defense response; Exopeptidases; Proteolysis; Wounding

Indexed keywords

AMINO ACID; CYTOSOL AMINOPEPTIDASE; HYBRID PROTEIN; MUTANT PROTEIN; ZINC ION;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME STABILITY; ESCHERICHIA COLI; MOLECULAR MODEL; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN HYDROLYSIS; TOMATO; WILD TYPE;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; DNA PRIMERS; HYDROLYSIS; KINETICS; LEUCYL AMINOPEPTIDASE; LYCOPERSICON ESCULENTUM; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, QUATERNARY; SEQUENCE HOMOLOGY, AMINO ACID;

BOVINAE; ESCHERICHIA COLI; LYCOPERSICON ESCULENTUM;

EID: 0036229654     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2002.02795.x     Document Type: Article

References (42)

1. Structure and mechanism of bovine lens leucine aminopeptidase
2. Plant aminopeptidases: Occurrence, function and characterization
3. Structure and function of bovine lens aminopeptidase and comparison with homologous aminopeptidases
4. Over-expression, purification and biochemical characterization of the wound-induced leucine aminopeptidase of tomato
5. Leucine aminopeptidase (bovine lens). Stability and size of subunits
6. Leucine aminopeptidases: The ubiquity of LAP-N and the specificity of LAP-A
7. Leucine aminopeptidase RNAs, proteins and activities increase in response to water deficit, salinity and the wound signals - Systemin, methyl jasmonate, and abscisic acid
8. A complex array of proteins related to the multimeric leucine aminopeptidase of tomato
9. Expression of the Arabidopsis abi1-1 mutant allele inhibits proteinase inhibitor wound-induction in tomato
10. Nature and regulation of pistil-expressed genes in tomato
11. General roles of abscisic and jasmonic acids in gene activation as a result of mechanical wounding
12. Leucine aminopeptidase: An inducible component of the defense response in Lycopersicon esculentum (tomato)
13. The induction of tomato leucine aminopeptidase genes (LapA) after Pseudomonas syringae pv. tomato infection is primarily a wound response triggered by coronatine
14. A -308 deletion of the tomato LAP promoters is able to direct flower-specific and MeJA-induced expression in transgenic plants
15. Induction of wound response genes in tomato leaves by bestatin, an inhibitor of aminopeptidases
16. Specificity of the wound-induced leucine aminopeptidase (LAP-A) of tomato: Activity on dipeptide and tripeptide substrates
17. Molecular structure of leucine aminopeptidase at 2.7-Å resolution
18. Structure determination and refinement of bovine lens leucine aminopeptidase and its complex with bestatin
19. Re-refinement of the X-ray crystal structure of bovine lens leucine aminopeptidase complexed with bestatin
20. Transition state analogue L-leucinephosphonic acid bound to bovine lens leucine aminopeptidase: X-ray structure at 1.65 Å resolution in a new crystal form
21. Two-metal ion mechanism of bovine lens leucine aminopeptidase: Active site solvent structure and binding mode of L-leucinal, a gem-diolate transition state analogue, by X-ray crystallography
22. X-ray structure of aminopeptidase A from Escherichia coli and a model for the nucleoprotein complex in Xer site-specific recombination
23. A bicarbonate ion as a general base in the mechanism of peptide hydrolysis by dizinc leucine aminopeptidase
24. Metal binding stoichiometry and mechanism of metal ion modulation of the activity of porcine kidney leucine aminopeptidase
25. Efficient site-directed mutagenesis using uracil-containing DNA
26. Purification and characterization of leucine aminopeptidase from kidney bean cotyledons
27. A new method of peptidase assay and the separation of three leucylglycinases of renal tissues
28. Quantitation of microgram amounts of protein in two-dimensional polyacrylamide gel electrophoresis sample buffer
29. On the size of the active site in proteases. I. Papain
30. Classification of peptidases
31. Program DYNAFIT for the analysis of enzyme kinetic data: Application to HIV proteinase
32. Cleavage of structural proteins during assembly of the head of bacteriophage T4
33. Localization and post-translational processing of the wound-induced leucine aminopeptidase proteins of tomato
34. Leucine aminopeptidase from Arabidopsis thaliana - Molecular evidence for a phylogenetically conserved enzyme of protein turnover in higher plants
35. xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase
36. Characterization of the Rickettsia prowazekii pepA gene encoding leucine aminopeptidase
37. Isolation of bovine kidney leucine aminopeptidase cDNA comparison with lens enzyme and tissue-specific expression of two mRNAs
38. Leucine aminopeptidase - Bestatin inhibition and a model for enzyme-catalyzed peptide hydrolysis
39. X-ray crystallographic determination of structure of bovine lens leucine aminopeptidase complexed with amastatin formulation of a catalytic mechanism featuring a gem-diolate transition state
40. 2+ of the zinc metalloenzyme, amino acid composition, and sulfhydryl content
41. Purification and properties of an aminopeptidase from Escherichia coli