Overexpression of a cell wall enzyme reduces xyloglucan depolymerization and softening of transgenic tomato fruits

Journal of Agricultural and Food Chemistry

Volumn 58, Issue 9, 2010, Pages 5708-5713

  Miedes, Eva ^a   Herbers, Karin ^b   Sonnewald, Uwe ^c   Lorences, Ester P ^d  

^a UNIVERSITY OF VALENCIA   (Spain)

^b BASF SE   (Germany)

^c UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^d NONE

Author keywords

Cell wall; Fruit softening; Xyloglucan; Xyloglucan xyloglucosyltransferase

Indexed keywords

MESSENGER RNA; POLYSACCHARIDE; PRIMER DNA;

ARTICLE; CELL WALL; ENZYMOLOGY; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; TOMATO; TRANSGENIC PLANT;

BASE SEQUENCE; CELL WALL; DNA PRIMERS; LYCOPERSICON ESCULENTUM; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; POLYSACCHARIDES; RNA, MESSENGER;

LYCOPERSICON ESCULENTUM;

EID: 77952220898     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf100242z     Document Type: Article

References (33)

1. Brummell, D. A.; Harpster, M. H. Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants Plant Mol. Biol. 2001, 47, 311-340
2. Vicente, A. R.; Saladié, M.; Rose, J. K. C.; Labavitch, J,M. The linkage between cell wall metabolism and fruit softening, looking to the future J. Sci. Food Agr. 2007, 87, 1435-1448
3. Smith, C. J.; Watson, C. F.; Ray, J.; Bird, C. R.; Morris, P. C.; Schuch, W.; Ryerson, D. Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes Nature 1988, 334, 714-726
4. Robinson, R; Tomes, M. Ripening inhibitor, a gene with multiple effects on ripening Rep. Tomato Genet. Coop. 1968, 18, 36-37
5. Giovannoni, J. J.; DellaPenna, D.; Bennett, A. B.; Fischer, R. L. Expression of a chimeric polygalacturonase gene in transgenic rin (ripening inhibitor) tomato fruit results in a polyuronide degradation but not fruit softening Plant Cell. 1989, 1, 53-63
6. Maclachlan, G.; Brady, C. Endo-1;4-β-glucanase; xyloglucanase; and xyloglucan endo-transglycosylase activities versus potential substrates in ripening tomatoes Plant Physiol. 1994, 105, 965-974
7. Harpster, M. H.; Brummell, D. A.; Dunsmuir, P. Suppression of a ripening-related endo-1-4-β-glucanase in transgenic pepper fruit does not prevent depolymerization of cell wall polysaccharides during ripening Plant Mol. Biol. 2002a, 50, 345-355
8. Harpster, M. H.; Dawson, D. M.; Nevins, D. J.; Dunsmuir, P.; Brummell, D. A. Constitutive overexpression of a ripening-related pepper endo-1-4-β-glucanase in transgenic tomato fruit does not increase xyloglucan depolymerization or fruit softening Plant Mol. Biol. 2002b, 50, 357-369
9. Fry, S. C.; Smith, R. C.; Renwick, K. F.; Martin, D. J.; Hodge, S. K.; Matthews, K. J. Xyloglucan endotransglycosylase; a new wall-loosening enzyme activity from plants Biochem. J. 1992, 282, 821-828
10. Nishitani, K.; Tominaga, R. Endo-xyloglucan transferase; a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule J. Biol. Chem. 1992, 267, 21058-21064
11. Nishitani, K. The role of endoxyloglucan transferase in the organization of plant cell walls Int. Rev. Cytol. 1997, 173, 157-206
12. Rose, J. K. C.; Braam, J.; Fry, S. C.; Nishitani, K. The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis, current perspectives and a new unifying nomenclature Plant Cell Physiol. 2002, 43, 1421-1435
13. Fry, S. C. Tansley Review, Primary cell wall metabolism, tracking the careers of wall polymers in living plant cells New Phytol. 2004, 161, 641-675
14. Arrowsmith, D. A.; de Silva, J. Characterization of two tomato fruit-expressed cDNAs encoding xyloglucan endotransglycosylases Plant Mol. Biol. 1995, 28, 391-403
15. Ishimaru, M.; Kobayashi, K. Expression of a xyloglucan endo-transglycosulase gene is closely related to grape berry softening Plant Sci. 2002, 162, 621-628
16. Fonseca, S.; Monteiro, L.; Barreiro, M. G.; Pais, M. S. Expression of genes encoding cell wall modifying enzymes is induced by cold storage and reflects changes in pear fruit texture J. Exp. Bot. 2005, 56, 2029-2036
17. Lu, W.; Wang, Y.; Jiang, Y.; Li, J.; Liu, H.; Duan, X.; Song, L. Differential expression of litchi XET genes in relation to fruit growth Plant Physiol. Biochem. 2006, 44, 707-713
18. Saladie, M.; Rose, J. K.; Cosgrove, D. J.; Catala, C. Characterization of a new xyloglucan endotransglucosylase/hydrolase (XTH) from ripening tomato fruit and implications for the diverse modes of enzymic action Plant J. 2006, 47, 282-295
19. Miedes, E.; Lorences, E. P. Xyloglucan endotransglucosylase/hydrolases (XTHs) during tomato fruit growth and ripening J. Plant Physiol. 2009, 166, 489-498
20. Hiwasa, K.; Nakano, R.; Hashimoto, A.; Matsuzaki, M.; Murayama, H. European; Chinese and Japanese pear fruits exhibit differential softening characteristics during ripening J. Exp. Bot. 2004, 55, 2281-2290
21. Nishiyama, K.; Guis, M.; Rose, J. C.; Kubo, Y.; Bennet, K. A.; Wangjin, L.; Kato, K.; Ushijima, K.; Nakano, R.; Inaba, A.; Bouzayen, M.; Latche, A.; Pech, J. C.; Bennet, A. Ethylene regulation of fruit softening and cell wall disassembly in Charentais melon J. Exp. Bot. 2007, 58, 1281-1290
22. Herbers, K.; Lorences, E. P.; Barrachina, C.; Sonnewald, U. Functional characterisation of Nicotiana tabacum; xyloglucan endotransglycosylase (Nt XET-1), Generation of transgenic tobacco plants and changes in cell wall xyloglucan Planta. 2001, 212, 279-287
23. Ling, H. Q.; Kriseleit, D.; Ganal, M. W. Effect of ticarcillin potassium clavulanate on callus growth and shoot regeneration in Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum Mill) Plant Cell Reports 1998, 17, 843-847
24. Larionov, A.; Krause, A.; Miller, W. A. Standard curve based method for relative Real-Time PCR data processing BMC Bioinformatics 2005, 6, 62
25. Miedes, E.; Lorences, E. P. Apple (Malus domestica) and tomato (Lycopersicum esculentum) fruits cell-wall hemicelluloses and xyloglucan degradation during Penicillium expansum infection J. Agric. Food Chem. 2004, 52, 7957-63
26. Bradford, M. M. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 1976, 72, 248-252
27. Lorences, E. P.; Fry, S. C. Xyloglucan oligosaccharides with at least two R-D-xylose residues act as an acceptor for xyloglucan endotransglycosylase and promote the depolymerization of xyloglucan Physiol. Plant. 1993, 88, 105-12
28. Cutillas-Iturralde, A.; Zarra, I.; Lorences, E. P. Implication of persimmon fruit hemicellulose metabolism in the softening process Importance of xyloglucan endotransglycoxylase Physiol. Plant. 1994, 91, 169-176
29. Dubois, M.; Gilles, K. A.; Hamilton, J. K.; Rebers, P. A.; Smith, F. Colorimetric method for determination of sugars and related substances Anal. Chem. 1956, 28, 350-356
30. Kooiman, P. A method for the determination of amyloid in plant seeds Recl. Trav. Chim. Pays-Bas 1960, 79, 675-678
31. Nishitani, K.; Masuda, Y. Auxin-induced changes in the cell wall structure changes in the sugar compositions; intrinsic viscosity and molecular weight distributions of matrix polysaccharides of the epicotyl cell wall of Vigna angularis Physiol. Plant. 1981, 52, 482-494
32. Yokohama, R.; Nishitani, K. A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis Plant Cell Physiol. 2001, 42, 1025-1033
33. Sakurai, N.; Nevins, D. J. Changes in physical properties and cell wall polysaccharides of tomato (Lycopersicon esculentum) pericarp tissues Physiol. Plant. 1993, 89, 681-686