Sequential checkpoints govern substrate selection during cotranslational protein targeting

Science

Volumn 328, Issue 5979, 2010, Pages 757-760

  Zhang, Xin ^a   Rashid, Rumana ^a,b   Wang, Kai ^a,c   Shan, Shu Ou ^a  

^a California Institute of Technology   (United States)

^b Department of Molecular Medicine City of Hope ^*  (United States)

^c WongPartnership LLP   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

SIGNAL RECOGNITION PARTICLE;

BIOASSAY; COLIFORM BACTERIUM; PHYSIOLOGY; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL MEMBRANE; ENZYME SPECIFICITY; ESCHERICHIA COLI; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN TARGETING; PROTEIN TRANSPORT;

CELL MEMBRANE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLUORESCENCE RESONANCE ENERGY TRANSFER; GUANOSINE TRIPHOSPHATE; HYDROPHOBICITY; KINETICS; MODELS, BIOLOGICAL; PROTEIN BINDING; PROTEIN BIOSYNTHESIS; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; RIBOSOMES; SIGNAL RECOGNITION PARTICLE; THERMODYNAMICS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77952127782     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1186743     Document Type: Article

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31. We thank C. Schaffitzel and N. Ban for help with the purification of RNCs and Trigger factor; J. Luirink for plasmids encoding the phoA signal sequence variants; L. Randall for the plasmid encoding luciferase; B. Bukau and E. Deuerling for the plasmid encoding Trigger factor; F. Nataro for the plasmid encoding EspP; H. D. Bernstein for the strain HDB51; and R. J. Deshaies, A. Varshavsky, W. Zhong, N. Pierce, and the Shan laboratory for comments on the manuscript. This work was supported by NIH grant GM078024, and career awards from the Burroughs Wellcome Foundation, the Henry and Camille Dreyfus Foundation, the Arnold and Mabel Beckman Foundation, and the Packard Foundation to S.S.